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BMRB: 16338

2KJR

Solution NMR structure of the N-terminal Ubiquitin-like Domain from Tubulin-binding Cofactor B, CG11242, from Drosophila melanogaster. Northeast Structural Genomics Consortium Target FR629A (residues 8-92).

Authors

Nair, R., Ramelot, T.A., Cort, J.R., Shastry, R., Ciccosanti, C., Jiang, M., Rost, B., Swapna, G., Acton, T.B., Xiao, R., Everett, J.K., Montelione, G.T., Kennedy, M.A.

Assembly

CG11242

Entity

1. CG11242 (polymer, Thiol state: all free), 95 monomers, 10342.51 Da Detail

MGHHHHHHSH GKSDFIKVNV SNSHNDAVAF EVKLAKDLTV AQLKTKLEIL TGGCAGTMKV QVFKGDTCVS TMDNNDAQLG YYANSDGLRL HVVDS

Formula weight

10342.51 Da

Source organism

Drosophila melanogaster

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.5 %, Completeness: 89.2 %, Completeness (bb): 89.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	89.2 % (938 of 1051)	89.6 % (480 of 536)	88.8 % (365 of 411)	89.4 % (93 of 104)
Backbone	89.1 % (508 of 570)	89.4 % (177 of 198)	89.2 % (247 of 277)	88.4 % (84 of 95)
Sidechain	89.6 % (509 of 568)	89.6 % (303 of 338)	89.1 % (197 of 221)	100.0 % (9 of 9)
Aromatic	65.9 % (54 of 82)	65.9 % (27 of 41)	65.9 % (27 of 41)
Methyl	100.0 % (106 of 106)	100.0 % (53 of 53)	100.0 % (53 of 53)

1. CG11242

MGHHHHHHSH GKSDFIKVNV SNSHNDAVAF EVKLAKDLTV AQLKTKLEIL TGGCAGTMKV QVFKGDTCVS TMDNNDAQLG YYANSDGLRL HVVDS

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 (±.5) K, pH 6.0 (±.1)

#	Name	Isotope labeling	Concentration
1	MES	natural abundance	50 (±2.5) mM
2	arginine	natural abundance	50 (±2.5) mM
3	DTT	natural abundance	10 (±0.5) mM
4	sodium azide	natural abundance	0.02 (±0.001) %
5	entity	[U-100% 13C; U-100% 15N]	1.1 (±0.05) mM
6	DSS	natural abundance	50 (±2.5) uM
7	H2O	natural abundance	95 %
8	D2O	natural abundance	5 %

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 (±.5) K, pH 6.0 (±.1)

#	Name	Isotope labeling	Concentration
9	MES	natural abundance	50 (±2.5) mM
10	arginine	natural abundance	50 (±2.5) mM
11	DTT	natural abundance	10 (±0.5) mM
12	sodium azide	natural abundance	0.02 (±0.001) %
13	entity	[U-5% 13C; U-100% 15N]	1.0 (±0.05) mM
14	DSS	natural abundance	50 (±2.5) uM
15	H2O	natural abundance	95 %
16	D2O	natural abundance	5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.34 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.34 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.15 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2KJR, Strand ID: A Detail

Release date

2009-07-07

Citation

Solution NMR structure of the N-terminal Ubiquitin-like Domain from Tubulin-binding Cofactor B, CG11242, from Drosophila melanogaster. Northeast Structural Genomics Consortium Target FR629A (residues 8-92)
Ramelot, T.A., Cort, J.R., Shastry, R., Ciccosanti, C., Jiang, M., Nair, R., Rost, B., Swapna, G., Acton, T.B., Xiao, R., Everett, J.K., Montelione, G.T., Kennedy, M.A.
Not known

Related entities 1. CG11242, : 1 : 1 : 14 entities Detail

Experiments performed 16 experiments Detail

1 2D 1H-15N HSQC