BMRB: 16354

NMR solution structure of the N terminal domain of subunit E (E1-52) of A1AO ATP synthase from Methanocaldococcus jannaschii

Authors

Gayen, S., Balakrishna, A., Gruber, G.

Assembly

A1AO ATP synthase

Entity

1. A1AO ATP synthase (polymer, Thiol state: not present), 52 monomers, 5754.708 Da Detail

MAVKLMGVDK IKSKILDDAK AEANKIISEA EAEKAKILEK AKEEAEKRKA EI

Formula weight

5754.708 Da

Source organism

Methanocaldococcus jannaschii

Exptl. method

solution NMR

Refine. method

TORSION ANGLE DYNAMICS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 81.1 %, Completeness (bb): 97.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	81.1 % (502 of 619)	89.7 % (295 of 329)	65.4 % (155 of 237)	98.1 % (52 of 53)
Backbone	97.8 % (305 of 312)	94.3 % (99 of 105)	100.0 % (155 of 155)	98.1 % (51 of 52)
Sidechain	69.3 % (248 of 358)	87.5 % (196 of 224)	38.3 % (51 of 133)	100.0 % (1 of 1)
Methyl	65.6 % (42 of 64)	100.0 % (32 of 32)	31.3 % (10 of 32)

1. E(1-52)

MAVKLMGVDK IKSKILDDAK AEANKIISEA EAEKAKILEK AKEEAEKRKA EI

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Sample

Solvent system trifluoroethanol/water, Pressure 1 atm, Temperature 308 K, pH 6.5, Details 90% H2O / 10% D2O containing 25 mM NaH2PO4/Na2HPO4/ (pH 6.5) and 0.1% NaN3 in 60 % TFE.

#	Name	Isotope labeling	Type	Concentration
1	sodium phosphate	natural abundance		25 mM
2	D2O	natural abundance		10 %
3	sodium azide	natural abundance		0.1 mM
4	TFE	natural abundance		60 %
5	H2O	natural abundance		90 %

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Protein Blocks Logo

Calculated from 20 models in PDB: 2KK7, Strand ID: A Detail

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Release date

2009-09-28

Citation

NMR solution structure of the N-terminal domain of subunit E (E1-52) of A1AO ATP synthase from Methanocaldococcus jannaschii
Gayen, S., Balakrishna, A.M., Gruber, G.
J. Bioenerg. Biomembr. (2009), 41, 343-348, PubMed 19760172 , DOI 10.1007/s10863-009-9237-3 , Abstract

Related entities 1. A1AO ATP synthase, : 1 : 38 entities Detail

Experiments performed 5 experiments Detail