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BMRB: 16365

2KKM

Solution NMR structure of yeast protein YOR252W [residues 38-178]: Northeast Structural Genomics Consortium target YT654

Authors

Cort, J.R., Yee, A., Liu, C., Arrowsmith, C.H., Kennedy, M.A.

Assembly

YOR252W

Entity

1. YOR252W (polymer, Thiol state: all free), 141 monomers, 16648.73 Da Detail

MREDKIAAKK KLHQDKRVHE LARVKFMQDV VNSDTFKGQP IFDHAHTREF IQSFIERDDT ELDELKKKRR SNRPPSNRQV LLQQRRDQEL KEFKAGFLCP DLSDAKNMEF LRNWNGTFGL LNTLRLIRIN DKGEQVVGGN E

Formula weight

16648.73 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

solution NMR

Refine. method

molecular dynamics, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.9 %, Completeness: 75.4 %, Completeness (bb): 85.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	75.4 % (1312 of 1741)	69.9 % (649 of 928)	80.2 % (527 of 657)	87.2 % (136 of 156)
Backbone	85.8 % (719 of 838)	82.8 % (236 of 285)	87.5 % (364 of 416)	86.9 % (119 of 137)
Sidechain	69.0 % (716 of 1037)	64.2 % (413 of 643)	76.3 % (286 of 375)	89.5 % (17 of 19)
Aromatic	43.2 % (51 of 118)	47.5 % (28 of 59)	37.9 % (22 of 58)	100.0 % (1 of 1)
Methyl	99.2 % (129 of 130)	98.5 % (64 of 65)	100.0 % (65 of 65)

1. YOR252W

MREDKIAAKK KLHQDKRVHE LARVKFMQDV VNSDTFKGQP IFDHAHTREF IQSFIERDDT ELDELKKKRR SNRPPSNRQV LLQQRRDQEL KEFKAGFLCP DLSDAKNMEF LRNWNGTFGL LNTLRLIRIN DKGEQVVGGN E

Show sample condition

Sample #1

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 293 K, pH 7

#	Name	Isotope labeling	Concentration
1	YOR252W	[U-99% 13C; U-99% 15N]	0.6 mM
2	sodium chloride	natural abundance	300 mM
3	DTT	natural abundance	10 mM
4	benzamidine	natural abundance	1 mM
5	sodium azide	natural abundance	0.01 %
6	H2O	natural abundance	93 %
7	D2O	natural abundance	7 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 7

#	Name	Isotope labeling	Concentration
8	YOR252W	[U-99% 13C; U-99% 15N]	0.6 mM
9	sodium chloride	natural abundance	300 mM
10	DTT	natural abundance	10 mM
11	benzamidine	natural abundance	1 mM
12	sodium azide	natural abundance	0.01 %
13	D2O	natural abundance	100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.06 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.34 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2KKM, Strand ID: A Detail

Release date

2012-08-02

Citation

Solution NMR structure of yeast protein YOR252W
Cort, J.R., Yee, A., Liu, C., Arrowsmith, C.H., Kennedy, M.A.
Not known

Related entities 1. YOR252W, : 1 : 2 : 2 entities Detail

Interaction partners 1. YOR252W, : 9 interactors Detail

More details for 9 interactors identified by 6 citations

Experiments performed 15 experiments Detail

1 2D 1H-15N HSQC