BMRB: 16377

RpR325/RPA3574 from Rhodopseudomonas palustris

Authors

Kennedy, M.A., Ramelot, T.A., Wang, H., Ciccosanti, C., Jang, M., Nair, R., Rost, B., Swapna, G., Acton, T.B., Xiao, R., Everett, J.K., Montelione, G.T., Kennedy, M.A.

Assembly

RPA3574

Entity

1. RPA3574 (polymer, Thiol state: unknown), 73 monomers, 8097.867 Da Detail

MLVTINGEQR EVQSASVAAL MTELDCTDGH YAVALNYDVV PRGKWDETPV TAGDEIEILT PRQGGLEHHH HHH

Formula weight

8097.867 Da

Source organism

Rhodopseudomonas palustris

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 94.5 %, Completeness: 91.5 %, Completeness (bb): 92.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	91.5 % (732 of 800)	91.7 % (374 of 408)	91.1 % (288 of 316)	92.1 % (70 of 76)
Backbone	92.1 % (398 of 432)	91.9 % (137 of 149)	92.5 % (197 of 213)	91.4 % (64 of 70)
Sidechain	91.3 % (397 of 435)	91.5 % (237 of 259)	90.6 % (154 of 170)	100.0 % (6 of 6)
Aromatic	57.1 % (32 of 56)	57.1 % (16 of 28)	55.6 % (15 of 27)	100.0 % (1 of 1)
Methyl	98.9 % (87 of 88)	97.7 % (43 of 44)	100.0 % (44 of 44)

1. RPA3574

MLVTINGEQR EVQSASVAAL MTELDCTDGH YAVALNYDVV PRGKWDETPV TAGDEIEILT PRQGGLEHHH HHH

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1.0 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	RPA3574	[U-100% 13C; U-100% 15N]		0.92 mM
2	MES	natural abundance		20 mM
3	potassium chloride	natural abundance		200 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1.0 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
4	RPA3574	[U-5% 13C; U-99% 15N]		0.85 mM
5	MES	natural abundance		20 mM
6	potassium chloride	natural abundance		200 mM

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LACS Plot; CA

Referencing offset: -0.18 ppm, Outliers: 4 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.18 ppm, Outliers: 4 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.04 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.3 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2LEK, Strand ID: A Detail

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Release date

2015-04-22

Citation

RpR325/RPA3574 from Rhodopseudomonas palustris
Kennedy, M.A., Ramelot, T.A., Wang, H., Ciccosanti, C., Jang, M., Nair, R., Rost, B., Swapna, G., Acton, T.B., Xiao, R., Everett, J.K., Montelione, G.T., Kennedy, M.A.
Not known

Related entities 1. RPA3574, : 1 : 4 entities Detail

Experiments performed 12 experiments Detail