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BMRB: 16386

2KL7

Solution NMR Structure of the EGF-like 1 Domain of Human Fibulin-4. Northeast Structural Genomics Target HR6275.

Authors

Rossi, P., Chiang, Y., Anderson, S., Montelione, G.T.

Assembly

Human Fibulin-4

Entity

1. Human Fibulin-4 (polymer, Thiol state: all disulfide bound), 71 monomers, 7570.374 Da Detail

SDVNECLTIP EACKGEMKCI NHYGGYLCLP RSAAVINDLH GEGPPPPVPP AQHPNPCPPG YEPDDQDSCV D

Formula weight

7570.374 Da

Entity Connection

disulfide 3 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS6:SG	1:CYS69:SG
2	disulfide	sing	1:CYS13:SG	1:CYS28:SG
3	disulfide	sing	1:CYS19:SG	1:CYS57:SG

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.0 %, Completeness: 88.4 %, Completeness (bb): 90.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	88.4 % (675 of 764)	87.3 % (351 of 402)	88.6 % (264 of 298)	93.8 % (60 of 64)
Backbone	90.8 % (363 of 400)	91.9 % (124 of 135)	89.4 % (185 of 207)	93.1 % (54 of 58)
Sidechain	86.0 % (369 of 429)	85.0 % (227 of 267)	87.2 % (136 of 156)	100.0 % (6 of 6)
Aromatic	100.0 % (36 of 36)	100.0 % (18 of 18)	100.0 % (18 of 18)
Methyl	100.0 % (54 of 54)	100.0 % (27 of 27)	100.0 % (27 of 27)

1. EGF-like 1 Domain

SDVNECLTIP EACKGEMKCI NHYGGYLCLP RSAAVINDLH GEGPPPPVPP AQHPNPCPPG YEPDDQDSCV D

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity	[U-100% 13C; U-100% 15N]	0.6 mM
2	sodium chloride	natural abundance	150 mM
3	MES	natural abundance	20 mM
4	DSS	natural abundance	50 uM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	entity	[U-100% 15N]	0.3 mM
8	sodium chloride	natural abundance	150 mM
9	MES	natural abundance	20 mM
10	DSS	natural abundance	50 uM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.11 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.11 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.05 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.2 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2KL7, Strand ID: A Detail

Release date

2009-08-05

Citation

Solution NMR Structure of the EGF-like 1 Domain of Human Fibulin-4. Northeast Structural Genomics Target HR6275
Rossi, P., Chiang, Y., Anderson, S., Montelione, G.T.
Not known

Related entities 1. Human Fibulin-4, : 1 : 2 : 47 entities Detail

Interaction partners 1. Human Fibulin-4, : 62 interactors Detail

More details for 62 interactors identified by 14 citations

Experiments performed 17 experiments Detail

1 2D 1H-15N HSQC