BMRB: 16405

Solution Structure of the Tandem UIM Domain of Ataxin-3 Complexed with Ubiquitin

Authors

Zhou, C., Song, A., Lin, D., Hu, H.

Assembly

Ataxin-3/Ubiquitin complex

Entity

1. Ataxin-3/Ubiquitin complex (polymer, Thiol state: not present), 46 monomers, 5263.612 Da Detail

GSLDEDEEDL QRALALSRQE IDMEDEEADL RRAIQLSMQG SSRNLE

Formula weight

5263.612 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.5 %, Completeness: 72.4 %, Completeness (bb): 75.7 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	72.4 % (369 of 510)	71.9 % (194 of 270)	70.9 % (134 of 189)	80.4 % (41 of 51)
Backbone	75.7 % (209 of 276)	87.2 % (82 of 94)	63.2 % (86 of 136)	89.1 % (41 of 46)
Sidechain	72.3 % (201 of 278)	63.6 % (112 of 176)	91.8 % (89 of 97)	0.0 % (0 of 5)
Methyl	93.2 % (41 of 44)	90.9 % (20 of 22)	95.5 % (21 of 22)

1. Ataxin-3/Ubiquitin complex

GSLDEDEEDL QRALALSRQE IDMEDEEADL RRAIQLSMQG SSRNLE

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	entity	[U-100% 15N]		1 mM
2	sodium phosphate	natural abundance		20 mM
3	sodium chloride	natural abundance		100 mM
4	sodium azide	natural abundance		0.02 %
5	ubiquitin	natural abundance		4 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
6	entity	[U-100% 13C; U-100% 15N]		1 mM
7	sodium phosphate	natural abundance		20 mM
8	sodium chloride	natural abundance		100 mM
9	sodium azide	natural abundance		0.02 %
10	ubiquitin	natural abundance		4 mM

Hide sample codition

LACS Plot; CA

Referencing offset: -0.15 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.25144
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.10132

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: -0.15 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.25144
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.10132

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: -0.04 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.25144
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.10132

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 10 models in PDB: 2KLZ, Strand ID: A Detail

---

Release date

2012-08-02

Citation

Structural transformation of the tandem ubiquitin-interacting motifs in ataxin-3 and their cooperative interactions with ubiquitin chains
Song, A., Zhou, C., Peng, Y., Gao, X., Zhou, Z., Fu, Q., Hong, J., Lin, D., Hu, H.
PLoS One (2010), 5, e13202-e13202, PubMed 20949063 , DOI 10.1371/journal.pone.0013202 , Abstract

Related entities 1. Ataxin-3/Ubiquitin complex, : 1 : 20 entities Detail

Interaction partners 1. Ataxin-3/Ubiquitin complex, : 29 interactors Detail

Experiments performed 8 experiments Detail