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BMRB: 16413

2LJK

C17orf37 Human 9606 Eukaryota Matazoa Homo sapiens

Authors

Yang, C., Huang, L., Chang, Y., Shen, T., Hsu, C., Chang, C.

Assembly

C17orf37

Entity

1. C17orf37 (polymer, Thiol state: all free), 117 monomers, 12546.94 Da Detail

GSMSGEPGQT SVAPPPEEVE PGSGVRIVVE YCEPCGFEAT YLELASAVKE QYPGIEIESR LGGTGAFEIE INGQLVFSKL ENGGFPYEKD LIEAIRRASN GETLEKITNS RPPCVIL

Formula weight

12546.94 Da

Source organism

Exptl. method

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.7 %, Completeness: 90.6 %, Completeness (bb): 95.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	90.6 % (1181 of 1304)	90.7 % (617 of 680)	88.8 % (453 of 510)	97.4 % (111 of 114)
Backbone	95.7 % (653 of 682)	95.4 % (227 of 238)	95.5 % (322 of 337)	97.2 % (104 of 107)
Sidechain	86.5 % (627 of 725)	88.2 % (390 of 442)	83.3 % (230 of 276)	100.0 % (7 of 7)
Aromatic	80.6 % (58 of 72)	88.9 % (32 of 36)	72.2 % (26 of 36)
Methyl	96.8 % (120 of 124)	100.0 % (62 of 62)	93.5 % (58 of 62)

1. C17orf37

GSMSGEPGQT SVAPPPEEVE PGSGVRIVVE YCEPCGFEAT YLELASAVKE QYPGIEIESR LGGTGAFEIE INGQLVFSKL ENGGFPYEKD LIEAIRRASN GETLEKITNS RPPCVIL

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	C17orf37	[U-13C; U-15N]		0.1 ~ 0.3 mM
2	potassium phosphate	natural abundance		20 mM
3	H2O	natural abundance		90 %
4	D2O	natural abundance		10 %

LACS Plot; CA

Referencing offset: -0.27 ppm, Outliers: 1 Detail

LACS Plot; CB

Referencing offset: -0.27 ppm, Outliers: 1 Detail

LACS Plot; HA

Referencing offset: -0.01 ppm, Outliers: 1 Detail

LACS Plot; CO

Referencing offset: 0.21 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LJK, Strand ID: A Detail

Release date

2010-06-21

Citation

Resonance assignments of human C35 (C17orf37) protein, a novel tumor biomarker
Yang, C., Chang, C., Huang, L., Chang, Y., Shen, T., Hsu, C.
Biomol. NMR Assign. (2010), 4, 191-193, PubMed 20556552 , DOI 10.1007/s12104-010-9218-9 , Abstract

Related entities 1. C17orf37, : 1 : 2 : 8 entities Detail

Interaction partners 1. C17orf37, : 4 interactors Detail

More details for 4 interactors identified by 3 citations

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 600 MHz Equipped 5mm TCI (1H/13C/15N) with Z gradient cryogenic probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	C17orf37	[U-13C; U-15N]		0.1 ~ 0.3 mM
2	potassium phosphate	natural abundance		20 mM
3	H2O	natural abundance		90 %
4	D2O	natural abundance		10 %

2 3D CBCA(CO)NH

Instrument

Bruker Avance - 600 MHz Equipped 5mm TCI (1H/13C/15N) with Z gradient cryogenic probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	C17orf37	[U-13C; U-15N]		0.1 ~ 0.3 mM
2	potassium phosphate	natural abundance		20 mM
3	H2O	natural abundance		90 %
4	D2O	natural abundance		10 %

3 3D HNCO

Instrument

Bruker Avance - 600 MHz Equipped 5mm TCI (1H/13C/15N) with Z gradient cryogenic probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	C17orf37	[U-13C; U-15N]		0.1 ~ 0.3 mM
2	potassium phosphate	natural abundance		20 mM
3	H2O	natural abundance		90 %
4	D2O	natural abundance		10 %

4 3D HNCA

Instrument

Bruker Avance - 600 MHz Equipped 5mm TCI (1H/13C/15N) with Z gradient cryogenic probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	C17orf37	[U-13C; U-15N]		0.1 ~ 0.3 mM
2	potassium phosphate	natural abundance		20 mM
3	H2O	natural abundance		90 %
4	D2O	natural abundance		10 %

5 3D HNCACB

Instrument

Bruker Avance - 600 MHz Equipped 5mm TCI (1H/13C/15N) with Z gradient cryogenic probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	C17orf37	[U-13C; U-15N]		0.1 ~ 0.3 mM
2	potassium phosphate	natural abundance		20 mM
3	H2O	natural abundance		90 %
4	D2O	natural abundance		10 %

6 3D HBHA(CO)NH

Instrument

Bruker Avance - 600 MHz Equipped 5mm TCI (1H/13C/15N) with Z gradient cryogenic probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	C17orf37	[U-13C; U-15N]		0.1 ~ 0.3 mM
2	potassium phosphate	natural abundance		20 mM
3	H2O	natural abundance		90 %
4	D2O	natural abundance		10 %

7 3D H(CCO)NH

Instrument

Bruker Avance - 600 MHz Equipped 5mm TCI (1H/13C/15N) with Z gradient cryogenic probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	C17orf37	[U-13C; U-15N]		0.1 ~ 0.3 mM
2	potassium phosphate	natural abundance		20 mM
3	H2O	natural abundance		90 %
4	D2O	natural abundance		10 %

8 3D HCCH-TOCSY

Instrument

Bruker Avance - 600 MHz Equipped 5mm TCI (1H/13C/15N) with Z gradient cryogenic probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	C17orf37	[U-13C; U-15N]		0.1 ~ 0.3 mM
2	potassium phosphate	natural abundance		20 mM
3	H2O	natural abundance		90 %
4	D2O	natural abundance		10 %

9 3D 1H-15N NOESY

Instrument

Bruker Avance - 600 MHz Equipped 5mm TCI (1H/13C/15N) with Z gradient cryogenic probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	C17orf37	[U-13C; U-15N]		0.1 ~ 0.3 mM
2	potassium phosphate	natural abundance		20 mM
3	H2O	natural abundance		90 %
4	D2O	natural abundance		10 %

10 3D 1H-15N TOCSY

Instrument

Bruker Avance - 600 MHz Equipped 5mm TCI (1H/13C/15N) with Z gradient cryogenic probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	C17orf37	[U-13C; U-15N]		0.1 ~ 0.3 mM
2	potassium phosphate	natural abundance		20 mM
3	H2O	natural abundance		90 %
4	D2O	natural abundance		10 %

11 3D HCCH-COSY

Instrument

Bruker Avance - 600 MHz Equipped 5mm TCI (1H/13C/15N) with Z gradient cryogenic probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	C17orf37	[U-13C; U-15N]		0.1 ~ 0.3 mM
2	potassium phosphate	natural abundance		20 mM
3	H2O	natural abundance		90 %
4	D2O	natural abundance		10 %

12 3D HCACO

Instrument

Bruker Avance - 600 MHz Equipped 5mm TCI (1H/13C/15N) with Z gradient cryogenic probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	C17orf37	[U-13C; U-15N]		0.1 ~ 0.3 mM
2	potassium phosphate	natural abundance		20 mM
3	H2O	natural abundance		90 %
4	D2O	natural abundance		10 %

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