BMRBj - BMREntryMaster

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BMRB: 16425

2KM8

Interdomain RRM packing contributes to RNA recognition in the rna15, hrp1, anchor RNA 3' processing ternary complex

Authors

Leeper, T.C., Varani, G.

Assembly

rna15, hrp1, anchor RNA 3' processing ternary complex

Entity

1. rna15p (polymer, Thiol state: all free), 84 monomers, 9342.414 Da Detail

NPPSRVVYLG SIPYDQTEEQ ILDLCSNVGP VINLKMMFDP QTGRSKGYAF IEFRDLESSA SAVRNLNGYQ LGSRFLKCGY SSNS

2. anchor RNA (polymer, Thiol state: not present), 13 monomers, 4159.450 Da Detail

UAUAUAUAAU AAU

3. hrp1p (polymer, Thiol state: all free), 167 monomers, 19035.30 Da Detail

KESCKMFIGG LNWDTTEDNL REYFGKYGTV TDLKIMKDPA TGRSRGFGFL SFEKPSSVDE VVKTQHILDG KVIDPKRAIP RDEQDKTGKI FVGGIGPDVR PKEFEEFFSQ WGTIIDAQLM LDKDTGQSRG FGFVTYDSAD AVDRVCQNKF IDFKDRKIEI KRAEPRH

Total weight

32537.164 Da

Max. entity weight

19035.3 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 34.5 %, Completeness: 27.6 %, Completeness (bb): 30.3 % Detail

Polymer type: polypeptide(L) polyribonucleotide

	Total	¹H	¹³C	¹⁵N
All	27.6 % (873 of 3166)	29.0 % (482 of 1662)	24.0 % (297 of 1238)	35.3 % (94 of 266)
Backbone	30.3 % (493 of 1625)	29.4 % (174 of 591)	29.6 % (235 of 795)	35.1 % (84 of 239)
Sidechain	25.6 % (452 of 1769)	28.9 % (309 of 1071)	19.8 % (133 of 671)	37.0 % (10 of 27)
Aromatic	10.0 % (33 of 330)	20.0 % (33 of 165)	0.0 % (0 of 157)	0.0 % (0 of 8)
Methyl	33.2 % (75 of 226)	36.3 % (41 of 113)	30.1 % (34 of 113)

1. rna15p

NPPSRVVYLG SIPYDQTEEQ ILDLCSNVGP VINLKMMFDP QTGRSKGYAF IEFRDLESSA SAVRNLNGYQ LGSRFLKCGY SSNS

2. anchor RNA

UAUAUAUAAU AAU

3. hrp1p

KESCKMFIGG LNWDTTEDNL REYFGKYGTV TDLKIMKDPA TGRSRGFGFL SFEKPSSVDE VVKTQHILDG KVIDPKRAIP RDEQDKTGKI FVGGIGPDVR PKEFEEFFSQ WGTIIDAQLM LDKDTGQSRG FGFVTYDSAD AVDRVCQNKF IDFKDRKIEI KRAEPRH

Show sample condition

Sample #1

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7 (±0.03)

#	Name	Isotope labeling	Concentration
1	rna15p	[U-100% 15N]	0.4 (±0.04) mM
2	hrp1p	natural abundance	0.4 (±0.04) mM
3	anchor RNA	natural abundance	0.4 (±0.04) mM
4	H2O	natural abundance	93 %
5	D2O	natural abundance	7 %
6	NaCl	natural abundance	150 mM
7	Phosphate	natural abundance	20 mM

Sample #2

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7 (±0.03)

#	Name	Isotope labeling	Concentration
8	rna15p	natural abundance	0.6 (±0.02) mM
9	hrp1p	[U-100% 15N]	0.6 (±0.07) mM
10	anchor RNA	natural abundance	0.6 (±0.03) mM
11	H2O	natural abundance	93 %
12	D2O	natural abundance	7 %
13	NaCl	natural abundance	150 mM
14	Phosphate	natural abundance	20 mM

Sample #3

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7 (±0.03)

#	Name	Isotope labeling	Concentration
15	rna15p	[U-100% 13C]	0.35 (±0.03) mM
16	hrp1p	[U-100% 15N]	0.35 (±0.03) mM
17	anchor RNA	natural abundance	0.35 (±0.03) mM
18	H2O	natural abundance	93 %
19	D2O	natural abundance	7 %
20	NaCl	natural abundance	150 mM
21	Phosphate	natural abundance	20 mM

Sample #4

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7 (±0.03)

#	Name	Isotope labeling	Concentration
22	rna15p	[U-100% 15N]	0.5 (±0.05) mM
23	hrp1p	[U-100% 13C]	0.5 (±0.05) mM
24	anchor RNA	natural abundance	0.5 (±0.05) mM
25	H2O	natural abundance	93 %
26	D2O	natural abundance	7 %
27	NaCl	natural abundance	150 mM
28	Phosphate	natural abundance	20 mM

Sample #5

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7 (±0.03)

#	Name	Isotope labeling	Concentration
29	rna15p	[U-100% 13C; U-100% 15N]	0.5 (±0.04) mM
30	hrp1p	natural abundance	0.5 (±0.04) mM
31	anchor RNA	natural abundance	0.5 (±0.04) mM
32	H2O	natural abundance	93 %
33	D2O	natural abundance	7 %
34	NaCl	natural abundance	150 mM
35	Phosphate	natural abundance	20 mM

Sample #6

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7 (±0.03)

#	Name	Isotope labeling	Concentration
36	rna15p	natural abundance	0.5 (±0.02) mM
37	hrp1p	[U-100% 13C; U-100% 15N]	0.5 (±0.02) mM
38	anchor RNA	natural abundance	0.5 (±0.02) mM
39	H2O	natural abundance	93 %
40	D2O	natural abundance	7 %
41	NaCl	natural abundance	150 mM
42	Phosphate	natural abundance	20 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
¹H	water	protons	4.71 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

Referencing offset: -0.08 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
¹H	water	protons	4.71 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

Referencing offset: -0.08 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
¹H	water	protons	4.71 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

Referencing offset: 0.08 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
¹H	water	protons	4.71 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

Referencing offset: -0.22 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2KM8, Strand ID: B, C Detail

Release date

2010-07-26

Citation

Novel protein-protein contacts facilitate mRNA 3'-processing signal recognition by Rna15 and Hrp1
Leeper, T.C., Qu, X., Lu, C., Moore, C., Varani, G.
J. Mol. Biol. (2010), 401, 334-349, PubMed 20600122 , DOI 10.1016/j.jmb.2010.06.032 , Abstract

Related entities 1. rna15p, : 1 : 2 : 3 : 347 entities Detail

Related entities 2. anchor RNA, : 1 : 6 entities Detail

Related entities 3. hrp1p, : 1 : 2 : 245 entities Detail

Interaction partners 1. rna15p, : 11 interactors Detail

More details for 11 interactors identified by 4 citations

Experiments performed 18 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 750 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7 (±0.03)

#	Name	Isotope labeling	Concentration
1	rna15p	[U-100% 15N]	0.4 (±0.04) mM
2	hrp1p	natural abundance	0.4 (±0.04) mM
3	anchor RNA	natural abundance	0.4 (±0.04) mM
4	H2O	natural abundance	93 %
5	D2O	natural abundance	7 %
6	NaCl	natural abundance	150 mM
7	Phosphate	natural abundance	20 mM

2 2D 1H-15N HSQC

Instrument

Bruker Avance - 750 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7 (±0.03)

#	Name	Isotope labeling	Concentration
8	rna15p	natural abundance	0.6 (±0.02) mM
9	hrp1p	[U-100% 15N]	0.6 (±0.07) mM
10	anchor RNA	natural abundance	0.6 (±0.03) mM
11	H2O	natural abundance	93 %
12	D2O	natural abundance	7 %
13	NaCl	natural abundance	150 mM
14	Phosphate	natural abundance	20 mM

3 2D 1H-15N HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State anisotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7 (±0.03)

#	Name	Isotope labeling	Concentration
15	rna15p	[U-100% 13C]	0.35 (±0.03) mM
16	hrp1p	[U-100% 15N]	0.35 (±0.03) mM
17	anchor RNA	natural abundance	0.35 (±0.03) mM
18	H2O	natural abundance	93 %
19	D2O	natural abundance	7 %
20	NaCl	natural abundance	150 mM
21	Phosphate	natural abundance	20 mM

4 2D 1H-15N HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State anisotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7 (±0.03)

#	Name	Isotope labeling	Concentration
22	rna15p	[U-100% 15N]	0.5 (±0.05) mM
23	hrp1p	[U-100% 13C]	0.5 (±0.05) mM
24	anchor RNA	natural abundance	0.5 (±0.05) mM
25	H2O	natural abundance	93 %
26	D2O	natural abundance	7 %
27	NaCl	natural abundance	150 mM
28	Phosphate	natural abundance	20 mM

5 2D 1H-13C HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State anisotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7 (±0.03)

#	Name	Isotope labeling	Concentration
15	rna15p	[U-100% 13C]	0.35 (±0.03) mM
16	hrp1p	[U-100% 15N]	0.35 (±0.03) mM
17	anchor RNA	natural abundance	0.35 (±0.03) mM
18	H2O	natural abundance	93 %
19	D2O	natural abundance	7 %
20	NaCl	natural abundance	150 mM
21	Phosphate	natural abundance	20 mM

6 2D 1H-13C HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State anisotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7 (±0.03)

#	Name	Isotope labeling	Concentration
22	rna15p	[U-100% 15N]	0.5 (±0.05) mM
23	hrp1p	[U-100% 13C]	0.5 (±0.05) mM
24	anchor RNA	natural abundance	0.5 (±0.05) mM
25	H2O	natural abundance	93 %
26	D2O	natural abundance	7 %
27	NaCl	natural abundance	150 mM
28	Phosphate	natural abundance	20 mM

7 2D 1H-13C HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7 (±0.03)

#	Name	Isotope labeling	Concentration
29	rna15p	[U-100% 13C; U-100% 15N]	0.5 (±0.04) mM
30	hrp1p	natural abundance	0.5 (±0.04) mM
31	anchor RNA	natural abundance	0.5 (±0.04) mM
32	H2O	natural abundance	93 %
33	D2O	natural abundance	7 %
34	NaCl	natural abundance	150 mM
35	Phosphate	natural abundance	20 mM

8 3D HNCO

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7 (±0.03)

#	Name	Isotope labeling	Concentration
29	rna15p	[U-100% 13C; U-100% 15N]	0.5 (±0.04) mM
30	hrp1p	natural abundance	0.5 (±0.04) mM
31	anchor RNA	natural abundance	0.5 (±0.04) mM
32	H2O	natural abundance	93 %
33	D2O	natural abundance	7 %
34	NaCl	natural abundance	150 mM
35	Phosphate	natural abundance	20 mM

9 3D HNCA

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7 (±0.03)

#	Name	Isotope labeling	Concentration
29	rna15p	[U-100% 13C; U-100% 15N]	0.5 (±0.04) mM
30	hrp1p	natural abundance	0.5 (±0.04) mM
31	anchor RNA	natural abundance	0.5 (±0.04) mM
32	H2O	natural abundance	93 %
33	D2O	natural abundance	7 %
34	NaCl	natural abundance	150 mM
35	Phosphate	natural abundance	20 mM

10 3D HNCACB

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7 (±0.03)

#	Name	Isotope labeling	Concentration
29	rna15p	[U-100% 13C; U-100% 15N]	0.5 (±0.04) mM
30	hrp1p	natural abundance	0.5 (±0.04) mM
31	anchor RNA	natural abundance	0.5 (±0.04) mM
32	H2O	natural abundance	93 %
33	D2O	natural abundance	7 %
34	NaCl	natural abundance	150 mM
35	Phosphate	natural abundance	20 mM

11 3D HN(CO)CA

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7 (±0.03)

#	Name	Isotope labeling	Concentration
29	rna15p	[U-100% 13C; U-100% 15N]	0.5 (±0.04) mM
30	hrp1p	natural abundance	0.5 (±0.04) mM
31	anchor RNA	natural abundance	0.5 (±0.04) mM
32	H2O	natural abundance	93 %
33	D2O	natural abundance	7 %
34	NaCl	natural abundance	150 mM
35	Phosphate	natural abundance	20 mM

12 3D CBCA(CO)NH

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7 (±0.03)

#	Name	Isotope labeling	Concentration
29	rna15p	[U-100% 13C; U-100% 15N]	0.5 (±0.04) mM
30	hrp1p	natural abundance	0.5 (±0.04) mM
31	anchor RNA	natural abundance	0.5 (±0.04) mM
32	H2O	natural abundance	93 %
33	D2O	natural abundance	7 %
34	NaCl	natural abundance	150 mM
35	Phosphate	natural abundance	20 mM

13 3D HCCH-TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7 (±0.03)

#	Name	Isotope labeling	Concentration
29	rna15p	[U-100% 13C; U-100% 15N]	0.5 (±0.04) mM
30	hrp1p	natural abundance	0.5 (±0.04) mM
31	anchor RNA	natural abundance	0.5 (±0.04) mM
32	H2O	natural abundance	93 %
33	D2O	natural abundance	7 %
34	NaCl	natural abundance	150 mM
35	Phosphate	natural abundance	20 mM

14 3D 1H-15N TOCSY

Instrument

Bruker Avance - 750 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7 (±0.03)

#	Name	Isotope labeling	Concentration
29	rna15p	[U-100% 13C; U-100% 15N]	0.5 (±0.04) mM
30	hrp1p	natural abundance	0.5 (±0.04) mM
31	anchor RNA	natural abundance	0.5 (±0.04) mM
32	H2O	natural abundance	93 %
33	D2O	natural abundance	7 %
34	NaCl	natural abundance	150 mM
35	Phosphate	natural abundance	20 mM

15 3D 1H-15N NOESY

Instrument

Bruker Avance - 750 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7 (±0.03)

#	Name	Isotope labeling	Concentration
29	rna15p	[U-100% 13C; U-100% 15N]	0.5 (±0.04) mM
30	hrp1p	natural abundance	0.5 (±0.04) mM
31	anchor RNA	natural abundance	0.5 (±0.04) mM
32	H2O	natural abundance	93 %
33	D2O	natural abundance	7 %
34	NaCl	natural abundance	150 mM
35	Phosphate	natural abundance	20 mM

16 3D 1H-13C NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7 (±0.03)

#	Name	Isotope labeling	Concentration
29	rna15p	[U-100% 13C; U-100% 15N]	0.5 (±0.04) mM
30	hrp1p	natural abundance	0.5 (±0.04) mM
31	anchor RNA	natural abundance	0.5 (±0.04) mM
32	H2O	natural abundance	93 %
33	D2O	natural abundance	7 %
34	NaCl	natural abundance	150 mM
35	Phosphate	natural abundance	20 mM

17 3D 1H-15N NOESY

Instrument

Bruker Avance - 750 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7 (±0.03)

#	Name	Isotope labeling	Concentration
36	rna15p	natural abundance	0.5 (±0.02) mM
37	hrp1p	[U-100% 13C; U-100% 15N]	0.5 (±0.02) mM
38	anchor RNA	natural abundance	0.5 (±0.02) mM
39	H2O	natural abundance	93 %
40	D2O	natural abundance	7 %
41	NaCl	natural abundance	150 mM
42	Phosphate	natural abundance	20 mM

18 3D 1H-13C NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7 (±0.03)

#	Name	Isotope labeling	Concentration
36	rna15p	natural abundance	0.5 (±0.02) mM
37	hrp1p	[U-100% 13C; U-100% 15N]	0.5 (±0.02) mM
38	anchor RNA	natural abundance	0.5 (±0.02) mM
39	H2O	natural abundance	93 %
40	D2O	natural abundance	7 %
41	NaCl	natural abundance	150 mM
42	Phosphate	natural abundance	20 mM

NMR combined restraints 8 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints, RDC restraints	combined_16425_2km8.nef
Input source #2: Coordindates	2km8.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

--------10---
UAUAUAUAAUAAU
|||||||||||||
UAUAUAUAAUAAU

Entity_assembly_ID (NMR): B vs Auth_asym_ID (model): B

-------30--------40--------50--------60--------70--------80--------90-------100-----
NPPSRVVYLGSIPYDQTEEQILDLCSNVGPVINLKMMFDPQTGRSKGYAFIEFRDLESSASAVRNLNGYQLGSRFLKCGYSSNS
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
NPPSRVVYLGSIPYDQTEEQILDLCSNVGPVINLKMMFDPQTGRSKGYAFIEFRDLESSASAVRNLNGYQLGSRFLKCGYSSNS
--------10--------20--------30--------40--------50--------60--------70--------80----

Entity_assembly_ID (NMR): C vs Auth_asym_ID (model): C

--160-------170-------180-------190-------200-------210-------220-------230-------240-------250-----
KESCKMFIGGLNWDTTEDNLREYFGKYGTVTDLKIMKDPATGRSRGFGFLSFEKPSSVDEVVKTQHILDGKVIDPKRAIPRDEQDKTGKIFVGGIGPDVR
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
KESCKMFIGGLNWDTTEDNLREYFGKYGTVTDLKIMKDPATGRSRGFGFLSFEKPSSVDEVVKTQHILDGKVIDPKRAIPRDEQDKTGKIFVGGIGPDVR
--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100

--260-------270-------280-------290-------300-------310-------320--
PKEFEEFFSQWGTIIDAQLMLDKDTGQSRGFGFVTYDSADAVDRVCQNKFIDFKDRKIEIKRAEPRH
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
PKEFEEFFSQWGTIIDAQLMLDKDTGQSRGFGFVTYDSADAVDRVCQNKFIDFKDRKIEIKRAEPRH
-------110-------120-------130-------140-------150-------160-------

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Sequence coverage (%)
A	A	13	100.0
B	B	84	100.0
C	C	167	100.0

Content subtype: combined_16425_2km8.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	840		100.0 (chain: B, length: 84)
1	Distance restraints	CNS/XPLOR_distance_constraints_2	Warning	7201 (1)	noe	100.0 (chain: A, length: 13), 98.8 (chain: B, length: 84), 98.8 (chain: C, length: 167)
2	Distance restraints	CNS/XPLOR_distance_constraints_7	Warning	150 (1)	noe	15.4 (chain: A, length: 13), 94.0 (chain: B, length: 84)
3	Distance restraints	CNS/XPLOR_distance_constraints_3	OK	132 (1)	hbond	42.9 (chain: B, length: 84), 40.1 (chain: C, length: 167)
1	Dihedral angle restraints	CNS/XPLOR_dihedral_4	OK	202 (202)	.	92.3 (chain: A, length: 13), 81.0 (chain: B, length: 84), 41.9 (chain: C, length: 167)
1	RDC restraints	CNS/XPLOR_dipolar_coupling_5	OK	151 (151)	.	64.3 (chain: B, length: 84), 57.5 (chain: C, length: 167)
2	RDC restraints	CNS/XPLOR_dipolar_coupling_6	Warning	50 (50)	.	20.2 (chain: B, length: 84), 16.8 (chain: C, length: 167)

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: B, Chain length: 84, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 472
  - ¹³C chemical shifts: 280
  - ¹⁵N chemical shifts: 88
- Completeness of assignments
  - Entity_assemble_ID: B
- Redox state of CYS
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: B

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	507	471	92.9
¹³C chemical shifts	374	280	74.9
¹⁵N chemical shifts	94	88	93.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	170	167	98.2
¹³C chemical shifts	168	157	93.5
¹⁵N chemical shifts	79	78	98.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	337	304	90.2
¹³C chemical shifts	206	123	59.7
¹⁵N chemical shifts	15	10	66.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	41	41	100.0
¹³C chemical shifts	41	34	82.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	40	33	82.5
¹³C chemical shifts	40	0	0.0

Distance restraints

Saveframe: CNS/XPLOR_distance_constraints_2
- Status: Warning
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 13, Sequence coverage: 100.0%
  - Entity_assembly_ID: B, Chain length: 84, Sequence coverage: 98.8%
  - Entity_assembly_ID: C, Chain length: 167, Sequence coverage: 98.8%
- Total number of restraints: 7191
- Weight of restraints: 1.0 (7191)
- Potential type of restraints: square-well-parabolic (7191)
- Range of distance target values: 2.45, 4.53 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
  - Chain_ID: B
  - Chain_ID: C
- Distance restraints on contact map
- Saveframe: CNS/XPLOR_distance_constraints_7
  - Status: Warning
  - Experiment type: noe
  - Sequence coverage of experimental data
    - Entity_assembly_ID: A, Chain length: 13, Sequence coverage: 15.4%
    - Entity_assembly_ID: B, Chain length: 84, Sequence coverage: 94.0%
  - Total number of restraints: 150
  - Weight of restraints: 1.0 (150)
  - Potential type of restraints: lower-bound-parabolic (82), square-well-parabolic (68)
  - Range of distance target values: 11.8, 16.8 (Å)
  - Histogram of distance target values
  - Distance restraints per residue
    - Chain_ID: A
    - Chain_ID: B
    - Chain_ID: C
      None
  - Distance restraints on contact map
  - Saveframe: CNS/XPLOR_distance_constraints_3
    - Status: OK
    - Experiment type: hbond
    - Sequence coverage of experimental data
      - Entity_assembly_ID: B, Chain length: 84, Sequence coverage: 42.9%
      - Entity_assembly_ID: C, Chain length: 167, Sequence coverage: 40.1%
    - Total number of restraints: 132
    - Weight of restraints: 1.0 (132)
    - Potential type of restraints: square-well-parabolic (132)
    - Range of distance target values: 1.95, 2.9 (Å)
    - Histogram of distance target values
    - Distance restraints per residue
      - Chain_ID: A
        None
      - Chain_ID: B
      - Chain_ID: C
    - Distance restraints on contact map

Dihedral angle restraints

Saveframe: CNS/XPLOR_dihedral_4
- Status: OK
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 13, Sequence coverage: 92.3%
  - Entity_assembly_ID: B, Chain length: 84, Sequence coverage: 81.0%
  - Entity_assembly_ID: C, Chain length: 167, Sequence coverage: 41.9%
- Total number of restraints: 202
- Total number of restraints per polymer type: 202
- Weight of restraints: 1.0 (202)
- Potential type of restraints: square-well-parabolic (202)
- Plot of Phi-Psi angle restraints
- Dihedral angle restraints per residue
  - Chain_ID: A
  - Chain_ID: B

RDC restraints

Saveframe: CNS/XPLOR_dipolar_coupling_5

Status: OK
Experiment type: .

Sequence coverage of experimental data

Entity_assembly_ID: B, Chain length: 84, Sequence coverage: 64.3%

-------30--------40--------50--------60--------70--------80--------90-------100-----
NPPSRVVYLGSIPYDQTEEQILDLCSNVGPVINLKMMFDPQTGRSKGYAFIEFRDLESSASAVRNLNGYQLGSRFLKCGYSSNS
   ||||       ||||||||||||||| || |||||        | ||||||||||||||||||||||    || || |   
...SRVV.......DQTEEQILDLCSNVG.VI.LKMMF........G.AFIEFRDLESSASAVRNLNGYQ....FL.CG.S   
-------30--------40--------50--------60--------70--------80--------90-------100--

Entity_assembly_ID: C, Chain length: 167, Sequence coverage: 57.5%

--160-------170-------180-------190-------200-------210-------220-------230-------240-------250-----
KESCKMFIGGLNWDTTEDNLREYFGKYGTVTDLKIMKDPATGRSRGFGFLSFEKPSSVDEVVKTQHILDGKVIDPKRAIPRDEQDKTGKIFVGGIGPDVR
    || ||       |||||||||||   ||||| |        || ||||||  |||||||||||     |  ||         ||| ||||    ||
....KM.IG.......EDNLREYFGKY...TDLKI.K........GF.FLSFEK..SVDEVVKTQHI.....I..KR.........TGK.FVGG....VR
--160-------170-------180-------190-------200-------210-------220-------230-------240-------250-----

--260-------270-------280-------290-------300-------310-------320--
PKEFEEFFSQWGTIIDAQLMLDKDTGQSRGFGFVTYDSADAVDRVCQNKFIDFKDRKIEIKRAEPRH
 |||||||||||||||||||       |  | ||||||||||||||||||      || |||     
.KEFEEFFSQWGTIIDAQLM.......S..F.FVTYDSADAVDRVCQNKF......KI.IKR     
--260-------270-------280-------290-------300-------310-------

Total number of restraints: 151
Potential type of restraints: undefined (151)
Range of observed RDC values: 100.0, -100.0 (Hz)
RDC restraints per residue
- Chain_ID: B
  None
- Chain_ID: C
  None
Saveframe: CNS/XPLOR_dipolar_coupling_6
- Status: Warning
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: B, Chain length: 84, Sequence coverage: 20.2%
  - Entity_assembly_ID: C, Chain length: 167, Sequence coverage: 16.8%
- Total number of restraints: 50
- Potential type of restraints: undefined (50)
- Range of observed RDC values: 100.0, -100.0 (Hz)
- RDC restraints per residue

Keywords 3' processing, enhancer element, hrp1p, NMR, positioning element, rna15p, RNA recognition, RRM domain

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BMRB: 16425

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Chemical shift reference

Chemical shift reference

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Chemical shift reference

Related entities 1. rna15p, : 1 : 2 : 3 : 347 entities Detail

Related entities 2. anchor RNA, : 1 : 6 entities Detail

Related entities 3. hrp1p, : 1 : 2 : 245 entities Detail

Interaction partners 1. rna15p, : 11 interactors Detail

Experiments performed 18 experiments Detail

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NMR combined restraints 8 contents Detail