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BMRB: 16426

2KMD

NMR-derived structure of residues 29-138 of murine Ets-1, containing the PNT domain, along with phosphorylated Thr38 and Ser41

Authors

Kang, H., Lee, G.M., Nelson, M.L., Graves, B.J., Blaszczak, A.G., McIntosh, L.P.

Assembly

murine Ets-1

Entity

1. murine Ets-1 (polymer, Thiol state: all free), 113 monomers, 12994.63 Da Detail

GSHMECADVP LLXPSXKEMM SQALKATFSG FTKEQQRLGI PKDPRQWTET HVRDWVMWAV NEFSLKGVDF QKFCMSGAAL CALGKECFLE LAPDFVGDIL WEHLEILQKE DVK

Formula weight

12994.63 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts, heteronucl_NOEs, heteronucl_T1_relaxation, heteronucl_T2_relaxation

Chem. Shift Complete

Sequence coverage: 96.5 %, Completeness: 95.9 %, Completeness (bb): 96.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	95.9 % (1273 of 1327)	95.4 % (659 of 691)	96.3 % (500 of 519)	97.4 % (114 of 117)
Backbone	96.0 % (630 of 656)	95.1 % (213 of 224)	96.6 % (315 of 326)	96.2 % (102 of 106)
Sidechain	94.3 % (731 of 775)	94.0 % (439 of 467)	94.6 % (281 of 297)	100.0 % (11 of 11)
Aromatic	93.8 % (122 of 130)	93.8 % (61 of 65)	93.4 % (57 of 61)	100.0 % (4 of 4)
Methyl	94.6 % (106 of 112)	92.9 % (52 of 56)	96.4 % (54 of 56)

1. murine Ets-1

GSHMECADVP LLXPSXKEMM SQALKATFSG FTKEQQRLGI PKDPRQWTET HVRDWVMWAV NEFSLKGVDF QKFCMSGAAL CALGKECFLE LAPDFVGDIL WEHLEILQKE DVK

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	2P-ETS1PNT	[U-100% 13C; U-100% 15N]	1 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.13 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.13 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.05 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.6 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2KMD, Strand ID: A Detail

Heteronucl. T₁

103 T₁ values in 1 lists
Coherence Sz, Field strength (¹H) 600 MHz, Pressure 1 atm, Temperature 303 K, pH 6.5 Detail

Heteronucl. T₂

103 T₂ values in 1 lists
Coherence S(+,-), Field strength (¹H) 600 MHz, Pressure 1 atm, Temperature 303 K, pH 6.5 Detail

Heteronucl. NOE

103 NOE values in 1 lists
Value type peak height, Field strength (¹H) 600 MHz, Pressure 1 atm, Temperature 303 K, pH 6.5 Detail

Heteronucl. T₁/T₂

103 T₁/T₂ values in 1 lists
Field strength (¹H) 600 MHz, Pressure 1 atm, Temperature 303 K, pH 6.5 Detail

Release date

2009-12-17

Citation

Detection and assignment of phosphoserine and phosphothreonine residues by (13)C- (31)P spin-echo difference NMR spectroscopy
McIntosh, L.P., Kang, H., Okon, M., Nelson, M.L., Graves, B.J., Brutscher, B.
J. Biomol. NMR (2009), 43, 31-37, PubMed 19002654 , DOI 10.1007/s10858-008-9287-6 , Abstract

Related entities 1. murine Ets-1, : 1 : 3 : 23 entities Detail

Interaction partners 1. murine Ets-1, : 5 interactors Detail

More details for 5 interactors identified by 3 citations

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC