BMRB: 16431

Complex of HIV-2 TAR RNA and a synthetic tripeptide in a 1:2 stoichiometry

Authors

Ferner, J., Suhartono, M., Breitung, S., Jonker, H.R.A., Hennig, M., Woehnert, J., Goebel, M., Schwalbe, H.

Assembly

TAR-2Lig

Entity

1. UUCG-TAR (polymer, Thiol state: not present), 28 monomers, 9003.263 Da Detail

GGCCAGAUUG AGCUUCGGCU CUCUGGUC

2. Pyrimidinylpeptide (polymer, Thiol state: not present), 3 monomers, 314.3874 × 2 Da

Total weight

9632.037 Da

Max. entity weight

9003.263 Da

Source organism

Human immunodeficiency virus 2

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.3 %, Completeness: 91.6 %, Completeness (bb): 92.2 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polyribonucleotide polypeptide(D)

	Total	1H	13C	15N
All	91.6 % (414 of 452)	92.7 % (230 of 248)	92.0 % (172 of 187)	70.6 % (12 of 17)
Backbone	92.2 % (284 of 308)	94.0 % (158 of 168)	90.0 % (126 of 140)
Sidechain	90.3 % (130 of 144)	90.0 % (72 of 80)	97.9 % (46 of 47)	70.6 % (12 of 17)
Aromatic	90.6 % (116 of 128)	90.6 % (58 of 64)	97.9 % (46 of 47)	70.6 % (12 of 17)

1. UUCG-TAR

GGCCAGAUUG AGCUUCGGCU CUCUGGUC

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.2, Details pH 6.2

#	Name	Isotope labeling	Type	Concentration
1	UUCG-TAR	[U-100% 13C; U-100% 15N]		0.65 mM
2	Pyrimidinylpeptide	natural abundance		5.2 mM
3	potassium chloride	natural abundance		50 mM
4	potassium phosphate	natural abundance		25 mM
5	H2O	natural abundance		90 %
6	D2O	natural abundance		10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.2, Details pH 6.2

#	Name	Isotope labeling	Type	Concentration
7	UUCG-TAR	[U-100% 13C; U-100% 15N]		0.65 mM
8	Pyrimidinylpeptide	natural abundance		5.2 mM
9	potassium chloride	natural abundance		50 mM
10	potassium phosphate	natural abundance		25 mM
11	D2O	natural abundance		100 %

Sample #3

Solvent system 90% H2O / 10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.2, Details pH 6.2

#	Name	Isotope labeling	Type	Concentration
12	UUCG-TAR	natural abundance		0.65 mM
13	Pyrimidinylpeptide	natural abundance		5.2 mM
14	potassium chloride	natural abundance		50 mM
15	potassium phosphate	natural abundance		25 mM
16	H2O	natural abundance		90 %
17	D2O	natural abundance		10 %

Sample #4

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.2, Details pH 6.2

#	Name	Isotope labeling	Type	Concentration
18	UUCG-TAR	natural abundance		0.65 mM
19	Pyrimidinylpeptide	natural abundance		5.2 mM
20	potassium chloride	natural abundance		50 mM
21	potassium phosphate	natural abundance		25 mM
22	D2O	natural abundance		100 %

Sample #5

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.2

#	Name	Isotope labeling	Type	Concentration
23	UUCG-TAR	[U-100% 13C; U-100% 15N]		0.2 mM
24	Pyrimidinylpeptide	natural abundance		0.8 mM
25	potassium chloride	natural abundance		50 mM
26	potassium phosphate	natural abundance		25 mM
27	Pf1 phage	natural abundance		16 mg/mL
28	D2O	natural abundance		100 %

Hide sample codition

Release date

2009-07-29

Citation

Structures of HIV TAR RNA-ligand complexes reveal higher binding stoichiometries
Ferner, J., Suhartono, M., Breitung, S., Jonker, H.R.A., Hennig, M., Woehnert, J., Goebel, M., Schwalbe, H.
Chembiochem. (2009), 10, 1490-1494, PubMed 19444830 , DOI 10.1002/cbic.200900220 , Abstract

Related entities 1. UUCG-TAR, : 1 : 2 entities Detail

Experiments performed 16 experiments Detail