CALMODULIN WRAPS AROUND ITS BINDING DOMAIN IN THE PLASMA MEMBRANE CA2+ PUMP ANCHORED BY A NOVEL 18-1 MOTIF
ADQLTEEQIA EFKEAFSLFD KDGDGTITTK ELGTVMRSLG QNPTEAELQD MINEVDADGN GTIDFPEFLT MMARKMKDTD SEEEIREAFR VFDKDGNGYI SAAELRHVMT NLGEKLTDEE VDEMIREADI DGDGQVNYEE FVQMMTAK
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 97.5 % (1985 of 2035) | 97.5 % (1034 of 1060) | 97.2 % (762 of 784) | 99.0 % (189 of 191) |
Backbone | 98.0 % (1031 of 1052) | 98.9 % (359 of 363) | 97.1 % (500 of 515) | 98.9 % (172 of 174) |
Sidechain | 97.3 % (1115 of 1146) | 96.8 % (675 of 697) | 97.9 % (423 of 432) | 100.0 % (17 of 17) |
Aromatic | 97.8 % (133 of 136) | 98.5 % (67 of 68) | 97.0 % (65 of 67) | 100.0 % (1 of 1) |
Methyl | 97.8 % (174 of 178) | 97.8 % (87 of 89) | 97.8 % (87 of 89) |
1. entity 1
ADQLTEEQIA EFKEAFSLFD KDGDGTITTK ELGTVMRSLG QNPTEAELQD MINEVDADGN GTIDFPEFLT MMARKMKDTD SEEEIREAFR VFDKDGNGYI SAAELRHVMT NLGEKLTDEE VDEMIREADI DGDGQVNYEE FVQMMTAK2. entity 2
LRRGQILWFR GLNRIQTQIK VVKAFHSSSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 (±0.5) K, pH 7.5 (±0.05), Details 13C,15N labeled holoCaM in complex with unlabeled peptide C28
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | entity_1 | [U-99% 13C; U-99% 15N] | 1.0 ~ 2.0 mM | |
2 | entity_2 | [U-99% 13C; U-99% 15N] | 1 mM | |
3 | H2O | natural abundance | 95 % | |
4 | D2O | natural abundance | 5 % |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 (±0.5) K, pH 7.5 (±0.05), Details 13C,15N labeled peptide C28 in complex with unlabeled holo-CaM
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | entity_1 | [U-99% 13C; U-99% 15N] | 1.0 ~ 2.0 mM | |
6 | entity_2 | [U-99% 13C; U-99% 15N] | 1 mM | |
7 | H2O | natural abundance | 95 % | |
8 | D2O | natural abundance | 5 % |
Bruker Avance - 700 MHz
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 (±0.5) K, pH 7.5 (±0.05), Details 13C,15N labeled holoCaM in complex with unlabeled peptide C28
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | entity_1 | [U-99% 13C; U-99% 15N] | 1.0 ~ 2.0 mM | |
2 | entity_2 | [U-99% 13C; U-99% 15N] | 1 mM | |
3 | H2O | natural abundance | 95 % | |
4 | D2O | natural abundance | 5 % |
Bruker Avance - 700 MHz
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 (±0.5) K, pH 7.5 (±0.05), Details 13C,15N labeled peptide C28 in complex with unlabeled holo-CaM
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | entity_1 | [U-99% 13C; U-99% 15N] | 1.0 ~ 2.0 mM | |
6 | entity_2 | [U-99% 13C; U-99% 15N] | 1 mM | |
7 | H2O | natural abundance | 95 % | |
8 | D2O | natural abundance | 5 % |
Bruker Avance - 700 MHz
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 (±0.5) K, pH 7.5 (±0.05), Details 13C,15N labeled holoCaM in complex with unlabeled peptide C28
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | entity_1 | [U-99% 13C; U-99% 15N] | 1.0 ~ 2.0 mM | |
2 | entity_2 | [U-99% 13C; U-99% 15N] | 1 mM | |
3 | H2O | natural abundance | 95 % | |
4 | D2O | natural abundance | 5 % |
Bruker Avance - 700 MHz
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 (±0.5) K, pH 7.5 (±0.05), Details 13C,15N labeled peptide C28 in complex with unlabeled holo-CaM
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | entity_1 | [U-99% 13C; U-99% 15N] | 1.0 ~ 2.0 mM | |
6 | entity_2 | [U-99% 13C; U-99% 15N] | 1 mM | |
7 | H2O | natural abundance | 95 % | |
8 | D2O | natural abundance | 5 % |
Bruker Avance - 700 MHz
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 (±0.5) K, pH 7.5 (±0.05), Details 13C,15N labeled holoCaM in complex with unlabeled peptide C28
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | entity_1 | [U-99% 13C; U-99% 15N] | 1.0 ~ 2.0 mM | |
2 | entity_2 | [U-99% 13C; U-99% 15N] | 1 mM | |
3 | H2O | natural abundance | 95 % | |
4 | D2O | natural abundance | 5 % |
Bruker Avance - 700 MHz
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 (±0.5) K, pH 7.5 (±0.05), Details 13C,15N labeled peptide C28 in complex with unlabeled holo-CaM
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | entity_1 | [U-99% 13C; U-99% 15N] | 1.0 ~ 2.0 mM | |
6 | entity_2 | [U-99% 13C; U-99% 15N] | 1 mM | |
7 | H2O | natural abundance | 95 % | |
8 | D2O | natural abundance | 5 % |
Bruker Avance - 700 MHz
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 (±0.5) K, pH 7.5 (±0.05), Details 13C,15N labeled holoCaM in complex with unlabeled peptide C28
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | entity_1 | [U-99% 13C; U-99% 15N] | 1.0 ~ 2.0 mM | |
2 | entity_2 | [U-99% 13C; U-99% 15N] | 1 mM | |
3 | H2O | natural abundance | 95 % | |
4 | D2O | natural abundance | 5 % |
Bruker Avance - 700 MHz
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 (±0.5) K, pH 7.5 (±0.05), Details 13C,15N labeled peptide C28 in complex with unlabeled holo-CaM
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | entity_1 | [U-99% 13C; U-99% 15N] | 1.0 ~ 2.0 mM | |
6 | entity_2 | [U-99% 13C; U-99% 15N] | 1 mM | |
7 | H2O | natural abundance | 95 % | |
8 | D2O | natural abundance | 5 % |
Bruker Avance - 700 MHz
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 (±0.5) K, pH 7.5 (±0.05), Details 13C,15N labeled holoCaM in complex with unlabeled peptide C28
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | entity_1 | [U-99% 13C; U-99% 15N] | 1.0 ~ 2.0 mM | |
2 | entity_2 | [U-99% 13C; U-99% 15N] | 1 mM | |
3 | H2O | natural abundance | 95 % | |
4 | D2O | natural abundance | 5 % |
Bruker Avance - 700 MHz
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 (±0.5) K, pH 7.5 (±0.05), Details 13C,15N labeled peptide C28 in complex with unlabeled holo-CaM
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | entity_1 | [U-99% 13C; U-99% 15N] | 1.0 ~ 2.0 mM | |
6 | entity_2 | [U-99% 13C; U-99% 15N] | 1 mM | |
7 | H2O | natural abundance | 95 % | |
8 | D2O | natural abundance | 5 % |
Bruker Avance - 700 MHz
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 (±0.5) K, pH 7.5 (±0.05), Details 13C,15N labeled holoCaM in complex with unlabeled peptide C28
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | entity_1 | [U-99% 13C; U-99% 15N] | 1.0 ~ 2.0 mM | |
2 | entity_2 | [U-99% 13C; U-99% 15N] | 1 mM | |
3 | H2O | natural abundance | 95 % | |
4 | D2O | natural abundance | 5 % |
Bruker Avance - 700 MHz
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 (±0.5) K, pH 7.5 (±0.05), Details 13C,15N labeled peptide C28 in complex with unlabeled holo-CaM
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | entity_1 | [U-99% 13C; U-99% 15N] | 1.0 ~ 2.0 mM | |
6 | entity_2 | [U-99% 13C; U-99% 15N] | 1 mM | |
7 | H2O | natural abundance | 95 % | |
8 | D2O | natural abundance | 5 % |
Bruker Avance - 700 MHz
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 (±0.5) K, pH 7.5 (±0.05), Details 13C,15N labeled holoCaM in complex with unlabeled peptide C28
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | entity_1 | [U-99% 13C; U-99% 15N] | 1.0 ~ 2.0 mM | |
2 | entity_2 | [U-99% 13C; U-99% 15N] | 1 mM | |
3 | H2O | natural abundance | 95 % | |
4 | D2O | natural abundance | 5 % |
Bruker Avance - 700 MHz
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 (±0.5) K, pH 7.5 (±0.05), Details 13C,15N labeled peptide C28 in complex with unlabeled holo-CaM
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | entity_1 | [U-99% 13C; U-99% 15N] | 1.0 ~ 2.0 mM | |
6 | entity_2 | [U-99% 13C; U-99% 15N] | 1 mM | |
7 | H2O | natural abundance | 95 % | |
8 | D2O | natural abundance | 5 % |
Bruker Avance - 700 MHz
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 (±0.5) K, pH 7.5 (±0.05), Details 13C,15N labeled holoCaM in complex with unlabeled peptide C28
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | entity_1 | [U-99% 13C; U-99% 15N] | 1.0 ~ 2.0 mM | |
2 | entity_2 | [U-99% 13C; U-99% 15N] | 1 mM | |
3 | H2O | natural abundance | 95 % | |
4 | D2O | natural abundance | 5 % |
Bruker Avance - 700 MHz
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 (±0.5) K, pH 7.5 (±0.05), Details 13C,15N labeled peptide C28 in complex with unlabeled holo-CaM
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | entity_1 | [U-99% 13C; U-99% 15N] | 1.0 ~ 2.0 mM | |
6 | entity_2 | [U-99% 13C; U-99% 15N] | 1 mM | |
7 | H2O | natural abundance | 95 % | |
8 | D2O | natural abundance | 5 % |
Bruker Avance - 700 MHz
State anisotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 (±0.5) K, pH 7.5 (±0.05), Details 13C,15N labeled holoCaM in complex with unlabeled peptide C28
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | entity_1 | [U-99% 13C; U-99% 15N] | 1.0 ~ 2.0 mM | |
2 | entity_2 | [U-99% 13C; U-99% 15N] | 1 mM | |
3 | H2O | natural abundance | 95 % | |
4 | D2O | natural abundance | 5 % |
Bruker Avance - 700 MHz
State anisotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 (±0.5) K, pH 7.5 (±0.05), Details 13C,15N labeled peptide C28 in complex with unlabeled holo-CaM
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | entity_1 | [U-99% 13C; U-99% 15N] | 1.0 ~ 2.0 mM | |
6 | entity_2 | [U-99% 13C; U-99% 15N] | 1 mM | |
7 | H2O | natural abundance | 95 % | |
8 | D2O | natural abundance | 5 % |
Bruker Avance - 700 MHz
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 (±0.5) K, pH 7.5 (±0.05), Details 13C,15N labeled holoCaM in complex with unlabeled peptide C28
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | entity_1 | [U-99% 13C; U-99% 15N] | 1.0 ~ 2.0 mM | |
2 | entity_2 | [U-99% 13C; U-99% 15N] | 1 mM | |
3 | H2O | natural abundance | 95 % | |
4 | D2O | natural abundance | 5 % |
Bruker Avance - 700 MHz
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 (±0.5) K, pH 7.5 (±0.05), Details 13C,15N labeled peptide C28 in complex with unlabeled holo-CaM
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | entity_1 | [U-99% 13C; U-99% 15N] | 1.0 ~ 2.0 mM | |
6 | entity_2 | [U-99% 13C; U-99% 15N] | 1 mM | |
7 | H2O | natural abundance | 95 % | |
8 | D2O | natural abundance | 5 % |
Properties
Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints, RDC restraints | combined_16465_2kne.nef |
Input source #2: Coordindates | 2kne.cif |
Diamagnetism of the molecular assembly | False (excluding Oxygen atoms) |
Whether the assembly has a disulfide bond | None |
Whether the assembly has a other bond | True (see coodinates for details) |
Whether the assembly contains a cyclic polymer | None |
Overall data processing status | Error |
Disulfide bonds
NoneOther bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)
Ptnr_site_1 | Ptnr_site_2 | Redox_state_prediction_1 | Redox_state_prediction_2 | Distance (Ã…) |
---|---|---|---|---|
3:1:CA:CA | 1:26:THR:O | unknown | unknown | n/a |
3:1:CA:CA | 1:22:ASP:OD1 | unknown | unknown | n/a |
3:1:CA:CA | 1:24:ASP:OD1 | unknown | unknown | n/a |
3:1:CA:CA | 1:20:ASP:OD1 | unknown | unknown | n/a |
3:1:CA:CA | 1:31:GLU:OE1 | unknown | unknown | n/a |
3:1:CA:CA | 1:31:GLU:OE2 | unknown | unknown | n/a |
3:2:CA:CA | 1:67:GLU:OE1 | unknown | unknown | n/a |
3:2:CA:CA | 1:67:GLU:OE2 | unknown | unknown | n/a |
3:2:CA:CA | 1:56:ASP:OD1 | unknown | unknown | n/a |
3:2:CA:CA | 1:58:ASP:OD1 | unknown | unknown | n/a |
3:2:CA:CA | 1:60:ASN:OD1 | unknown | unknown | n/a |
3:2:CA:CA | 1:62:THR:O | unknown | unknown | n/a |
3:3:CA:CA | 1:104:GLU:OE1 | unknown | unknown | n/a |
3:3:CA:CA | 1:97:ASN:OD1 | unknown | unknown | n/a |
3:3:CA:CA | 1:99:TYR:O | unknown | unknown | n/a |
3:3:CA:CA | 1:95:ASP:OD1 | unknown | unknown | n/a |
3:3:CA:CA | 1:104:GLU:OE2 | unknown | unknown | n/a |
3:3:CA:CA | 1:93:ASP:OD1 | unknown | unknown | n/a |
3:4:CA:CA | 1:140:GLU:OE1 | unknown | unknown | n/a |
3:4:CA:CA | 1:140:GLU:OE2 | unknown | unknown | n/a |
3:4:CA:CA | 1:129:ASP:OD1 | unknown | unknown | n/a |
3:4:CA:CA | 1:133:ASP:OD1 | unknown | unknown | n/a |
3:4:CA:CA | 1:135:GLN:O | unknown | unknown | n/a |
3:4:CA:CA | 1:131:ASP:OD1 | unknown | unknown | n/a |
Non-standard residues
Chain_ID | Seq_ID | Comp_ID | Chem_comp_name | Experimental evidences |
---|---|---|---|---|
C | 1 | CA | CALCIUM ION | Distance restraints |
C | 2 | CA | CALCIUM ION | None |
C | 3 | CA | CALCIUM ION | None |
C | 4 | CA | CALCIUM ION | None |
Sequence alignments
--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100 ADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYI |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| ADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYI -------110-------120-------130-------140-------- SAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK |||||||||||||||||||||||||||||||||||||||||||||||| SAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK
----1090---1100------1110--- LRRGQILWFRGLNRIQTQIKVVKAFHSS |||||||||||||||||||||||||||| LRRGQILWFRGLNRIQTQIKVVKAFHSS --------10--------20--------
Chain assignments
Entity_assembly_ID (NMR) | Auth_asym_ID (model) | Length | Unmapped | Conflict | Sequence coverage (%) |
---|---|---|---|---|---|
A | A | 148 | 0 | 0 | 100.0 |
B | B | 28 | 0 | 0 | 100.0 |
Content subtype: combined_16465_2kne.nef
Assigned chemical shifts
--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100 ADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYI |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| ADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYI -------110-------120-------130-------140-------- SAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK |||||||||||||||||||||||||||||||||||||||||||||||| SAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK
----1090---1100------1110--- LRRGQILWFRGLNRIQTQIKVVKAFHSS |||||||||||||||||||||||||| ..RGQILWFRGLNRIQTQIKVVKAFHSS
Comp_index_ID | Comp_ID | Atom_ID | CS value (ppm) |
---|---|---|---|
107 | HIS | HD1 | 8.66 |
107 | HIS | ND1 | 119.3 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 867 | 859 | 99.1 |
13C chemical shifts | 645 | 633 | 98.1 |
15N chemical shifts | 164 | 158 | 96.3 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 305 | 304 | 99.7 |
13C chemical shifts | 296 | 285 | 96.3 |
15N chemical shifts | 146 | 146 | 100.0 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 562 | 555 | 98.8 |
13C chemical shifts | 349 | 348 | 99.7 |
15N chemical shifts | 18 | 12 | 66.7 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 80 | 80 | 100.0 |
13C chemical shifts | 80 | 80 | 100.0 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 50 | 49 | 98.0 |
13C chemical shifts | 50 | 49 | 98.0 |
Comp_index_ID | Comp_ID | Atom_ID | CS value (ppm) |
---|---|---|---|
1111 | HIS | HD1 | 9.08 |
1111 | HIS | ND1 | 124.7 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 193 | 174 | 90.2 |
13C chemical shifts | 139 | 125 | 89.9 |
15N chemical shifts | 37 | 31 | 83.8 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 58 | 54 | 93.1 |
13C chemical shifts | 56 | 50 | 89.3 |
15N chemical shifts | 28 | 26 | 92.9 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 135 | 120 | 88.9 |
13C chemical shifts | 83 | 75 | 90.4 |
15N chemical shifts | 9 | 5 | 55.6 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 18 | 16 | 88.9 |
13C chemical shifts | 18 | 16 | 88.9 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 18 | 18 | 100.0 |
13C chemical shifts | 17 | 16 | 94.1 |
15N chemical shifts | 1 | 1 | 100.0 |
Covalent bonds
Distance restraints
--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100 ADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYI |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| ADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYI -------110-------120-------130-------140-------- SAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK |||||||||||||||||||||||||||||||||||||||||||||||| SAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK
----1090---1100------1110--- LRRGQILWFRGLNRIQTQIKVVKAFHSS |||||||||||||||||||||||||| ..RGQILWFRGLNRIQTQIKVVKAFHSS
--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100 ADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYI |||||||||||||||| ||||||||||||| |||||||||||| ||||||||||||| |||||||||| | ....TEEQIAEFKEAFSLFD......ITTKELGTVMRSL.....EAELQDMINEVD......IDFPEFLTMMARK......EEEIREAFRV........I --------10--------20--------30--------40--------50--------60--------70--------80--------90-------100 -------110-------120-------130-------140-------- SAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK ||||||||||| |||||||||||| |||||||||| SAAELRHVMTN......DEEVDEMIREAD......VNYEEFVQMM -------110-------120-------130-------140-----
----1090---1100------1110--- LRRGQILWFRGLNRIQTQIKVVKAFHSS ||||||||||||||||||||| ....QILWFRGLNRIQTQIKVVKAF ----1090---1100------1110
Dihedral angle restraints
--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100 ADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYI |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||| ...LTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMK..DSEEEIREAFRVFDKDGNGYI --------10--------20--------30--------40--------50--------60--------70--------80--------90-------100 -------110-------120-------130-------140-------- SAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK ||||||||||||||||||||||||||||||| |||||||||||||| SAAELRHVMTNLGEKLTDEEVDEMIREADID..GQVNYEEFVQMMTA -------110-------120-------130-------140-------
----1090---1100------1110--- LRRGQILWFRGLNRIQTQIKVVKAFHSS || |||||||||||||||||||||| ...GQ.LWFRGLNRIQTQIKVVKAFHSS
RDC restraints
--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100 ADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYI ||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||||||| |||||||||||||||||||||||||||||||||| .DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQN.TEAELQDMINEVDADGNGTIDF.EFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYI -------110-------120-------130-------140-------- SAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK |||||||||||||||||||||||||||||||||||||||||||||||| SAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK
----1090---1100------1110--- LRRGQILWFRGLNRIQTQIKVVKAFHSS |||||||||||||||||||||||||| ..RGQILWFRGLNRIQTQIKVVKAFHSS