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BMRB: 16478

2KNA

Solution structure of UBA domain of XIAP

Authors

Hui, S., Tse, M., Sze, K.

Assembly

UBA

Entity

1. UBA (polymer, Thiol state: not present), 104 monomers, 11859.26 Da Detail

GSAMADIGSE FEKTPSLTRR IDDTIFQNPM VQEAIRMGFS FKDIKKIMEE KIQISGSNYK SLEVLVADLV NAQKDSMQDE SSQTSLQKEI STEEQLRRLQ EEKL

Formula weight

11859.26 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 96.8 %, Completeness (bb): 98.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	96.8 % (1195 of 1234)	97.2 % (634 of 652)	96.8 % (453 of 468)	94.7 % (108 of 114)
Backbone	98.9 % (613 of 620)	98.1 % (206 of 210)	99.7 % (307 of 308)	98.0 % (100 of 102)
Sidechain	95.5 % (682 of 714)	96.8 % (428 of 442)	94.6 % (246 of 260)	66.7 % (8 of 12)
Aromatic	70.8 % (34 of 48)	100.0 % (24 of 24)	41.7 % (10 of 24)
Methyl	100.0 % (104 of 104)	100.0 % (52 of 52)	100.0 % (52 of 52)

1. UBA

GSAMADIGSE FEKTPSLTRR IDDTIFQNPM VQEAIRMGFS FKDIKKIMEE KIQISGSNYK SLEVLVADLV NAQKDSMQDE SSQTSLQKEI STEEQLRRLQ EEKL

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.7

#	Name	Isotope labeling	Concentration
1	Bis-TRIS	natural abundance	20 mM
2	sodium chloride	natural abundance	100 mM
3	DTT	natural abundance	5 mM
4	PMSF	natural abundance	1 mM
5	UBA	[U-99% 13C; U-99% 15N]	0.5 mM
6	D2O	natural abundance	10 %
7	H2O	natural abundance	90 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.14 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.14 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.07 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 15 models in PDB: 2KNA, Strand ID: A Detail

Release date

2010-05-04

Citation

Backbone and side-chain 1H, 13C and 15N assignments of the ubiquitin-associated domain of human X-linked inhibitor of apoptosis protein
Hui, S., Tse, M., Yang, Y., Wong, B., Sze, K.
Biomol. NMR Assign. (2010), 4, 13-15, PubMed 19916060 , DOI 10.1007/s12104-009-9197-x , Abstract

Related entities 1. UBA, : 1 : 2 : 15 entities Detail

Interaction partners 1. UBA, : 73 interactors Detail

More details for 73 interactors identified by 25 citations

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC