BMRB: 16497

1H, 13C, and 15N Chemical Shift Assignments for the Integrin AlphaIIb-Beta3 Transmembrane Complex

Authors

Lau, T., Ulmer, T.S.

Assembly

Integrin aIIbb3 transmembrane complex

Entity

1. Integrin aIIb transmembrane segment, b3 complexed (polymer, Thiol state: not present), 42 monomers, 4746.790 Da Detail

GALEERAIPI WWVLVGVLGG LLLLTILVLA MWKVGFFKRN RP

2. Integrin b3 transmembrane segment, aIIb complexed (polymer, Thiol state: not present), 43 monomers, 4707.745 Da Detail

PESPKGPDIL VVLLSVMGAI LLIGLAALLI WKLLITIHDR KEF

Total weight

9454.535 Da

Max. entity weight

4746.79 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.8 %, Completeness: 60.8 %, Completeness (bb): 77.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	60.8 % (640 of 1052)	63.3 % (342 of 540)	52.5 % (224 of 427)	87.1 % (74 of 85)
Backbone	77.2 % (386 of 500)	90.2 % (156 of 173)	63.2 % (156 of 247)	92.5 % (74 of 80)
Sidechain	42.3 % (266 of 629)	50.7 % (186 of 367)	31.1 % (80 of 257)	0.0 % (0 of 5)
Aromatic	0.0 % (0 of 82)	0.0 % (0 of 41)	0.0 % (0 of 37)	0.0 % (0 of 4)
Methyl	83.1 % (133 of 160)	85.0 % (68 of 80)	81.3 % (65 of 80)

1. Integrin aIIb transmembrane segment, b3 complexed

GALEERAIPI WWVLVGVLGG LLLLTILVLA MWKVGFFKRN RP

2. Integrin b3 transmembrane segment, aIIb complexed

PESPKGPDIL VVLLSVMGAI LLIGLAALLI WKLLITIHDR KEF

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 301.2 K, pH 7.4

#	Name	Isotope labeling	Type	Concentration
1	Integrin aIIb transmembrane segment, b3 complexed	[U-100% 13C; U-100% 15N; U-80% 2H]		0.4 mM
2	Integrin b3 transmembrane segment, aIIb complexed	natural abundance		0.8 mM
3	HEPES/NaOH	natural abundance		25 mM
4	H2O	natural abundance		95 %
5	D2O	natural abundance		5 %

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 301.2 K, pH 7.4

#	Name	Isotope labeling	Type	Concentration
6	Integrin aIIb transmembrane segment, b3 complexed	natural abundance		0.9 mM
7	Integrin b3 transmembrane segment, aIIb complexed	[U-100% 13C; U-100% 15N; U-80% 2H]		0.6 mM
8	HEPES/NaOH	natural abundance		25 mM
9	H2O	natural abundance		95 %
10	D2O	natural abundance		5 %

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Protein Blocks Logo

Calculated from 21 models in PDB: 2K9J, Strand ID: A, B Detail

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Release date

2009-09-23

Citation

The structure of the integrin alphaIIbbeta3 transmembrane complex explains integrin transmembrane signalling
Lau, T., Kim, C., Ginsberg, M.H., Ulmer, T.S.
EMBO J. (2009), 28, 1351-1361, PubMed 19279667 , DOI 10.1038/emboj.2009.63 , Abstract

Related entities 1. Integrin aIIb transmembrane segment, b3 complexed, : 1 : 1 : 5 : 47 entities Detail

Related entities 2. Integrin b3 transmembrane segment, aIIb complexed, : 1 : 2 : 8 : 49 entities Detail

Interaction partners 1. Integrin aIIb transmembrane segment, b3 complexed, : 3 : 1 interactors Detail

Interaction partners 2. Integrin b3 transmembrane segment, aIIb complexed, : 41 : 1 interactors Detail

Experiments performed 8 experiments Detail