BMRBj - BMREntryMaster

Found 1 Document (0.638 seconds)

BMRB: 16521

2KON

NMR solution structure of CV_2116 from Chromobacterium violaceum.Northeast Structural Genomics Consortium Target CvT4(1-82)

Authors

Yang, Y., Ramelot, T.A., Cort, J.R., Garcia, M., Yee, A., Arrowsmith, C.H., Montelio, G.T., Kennedy, M.A.

Assembly

CV 2116

Entity

1. CV 2116 (polymer, Thiol state: not present), 82 monomers, 9429.494 Da Detail

MNVAHYRGYE IEPGHQYRDD IRKYVPYALI RKVGVPDRTP IPTTYPEFYD LEADAERVSI ACAKIIIDSH LDRHDQGLAD LG

Formula weight

9429.494 Da

Source organism

Chromobacterium violaceum

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 98.4 %, Completeness (bb): 97.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	98.4 % (937 of 952)	99.4 % (492 of 495)	97.4 % (368 of 378)	97.5 % (77 of 79)
Backbone	97.1 % (466 of 480)	98.8 % (161 of 163)	95.9 % (231 of 241)	97.4 % (74 of 76)
Sidechain	99.6 % (547 of 549)	99.7 % (331 of 332)	99.5 % (213 of 214)	100.0 % (3 of 3)
Aromatic	100.0 % (82 of 82)	100.0 % (41 of 41)	100.0 % (41 of 41)
Methyl	100.0 % (92 of 92)	100.0 % (46 of 46)	100.0 % (46 of 46)

1. CV 2116

MNVAHYRGYE IEPGHQYRDD IRKYVPYALI RKVGVPDRTP IPTTYPEFYD LEADAERVSI ACAKIIIDSH LDRHDQGLAD LG

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	CV_2116	[U-100% 13C; U-100% 15N]	0.8 (±0.1) mM
2	TRIS	natural abundance	10 (±0.5) mM
3	sodium chloride	natural abundance	300 (±15.0) mM
4	zinc sulfate	natural abundance	10 (±0.5) uM
5	DTT	natural abundance	10 (±0.5) mM
6	benzamidine	natural abundance	1 (±0.05) mM
7	inhibitor cocktail	natural abundance

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
8	CV_2116	natural abundance	0.8 (±0.1) mM
9	TRIS	natural abundance	10 (±0.5) mM
10	sodium chloride	natural abundance	300 (±15.0) mM
11	zinc sulfate	natural abundance	10 (±0.5) uM
12	DTT	natural abundance	10 (±0.5) mM
13	benzamidine	natural abundance	1 (±0.05) mM
14	inhibitor cocktail	natural abundance

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 293 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
15	CV_2116	[U-100% 13C; U-100% 15N]	0.8 (±0.1) mM
16	TRIS	natural abundance	10 (±0.5) mM
17	sodium chloride	natural abundance	300 (±15.0) mM
18	zinc sulfate	natural abundance	10 (±0.05) uM
19	DTT	natural abundance	10 (±0.5) mM
20	benzamidine	natural abundance	1 (±0.05) mM
21	inhibitor cocktail	natural abundance

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.22 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.22 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.08 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2KON, Strand ID: A Detail

Release date

2014-03-03

Citation

NMR solution structure of CV_2116 from Chromobacterium violaceum. Northeast Structural Genomics Consortium Target CvT4(1-82)
Yang, Y., Ramelot, T.A., Cort, J.R., Garcia, M., Yee, A., Arrowsmith, C.H., Montelione, G.T., Kennedy, M.A.
Not known

Related entities 1. CV 2116, : 1 entities Detail

Experiments performed 17 experiments Detail

1 2D 1H-15N HSQC

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	CV_2116	[U-100% 13C; U-100% 15N]	0.8 (±0.1) mM
2	TRIS	natural abundance	10 (±0.5) mM
3	sodium chloride	natural abundance	300 (±15.0) mM
4	zinc sulfate	natural abundance	10 (±0.5) uM
5	DTT	natural abundance	10 (±0.5) mM
6	benzamidine	natural abundance	1 (±0.05) mM
7	inhibitor cocktail	natural abundance

2 2D 1H-13C HSQC

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	CV_2116	[U-100% 13C; U-100% 15N]	0.8 (±0.1) mM
2	TRIS	natural abundance	10 (±0.5) mM
3	sodium chloride	natural abundance	300 (±15.0) mM
4	zinc sulfate	natural abundance	10 (±0.5) uM
5	DTT	natural abundance	10 (±0.5) mM
6	benzamidine	natural abundance	1 (±0.05) mM
7	inhibitor cocktail	natural abundance

3 2D 1H-13C HSQC

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
8	CV_2116	natural abundance	0.8 (±0.1) mM
9	TRIS	natural abundance	10 (±0.5) mM
10	sodium chloride	natural abundance	300 (±15.0) mM
11	zinc sulfate	natural abundance	10 (±0.5) uM
12	DTT	natural abundance	10 (±0.5) mM
13	benzamidine	natural abundance	1 (±0.05) mM
14	inhibitor cocktail	natural abundance

4 3D 1H-15N NOESY

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	CV_2116	[U-100% 13C; U-100% 15N]	0.8 (±0.1) mM
2	TRIS	natural abundance	10 (±0.5) mM
3	sodium chloride	natural abundance	300 (±15.0) mM
4	zinc sulfate	natural abundance	10 (±0.5) uM
5	DTT	natural abundance	10 (±0.5) mM
6	benzamidine	natural abundance	1 (±0.05) mM
7	inhibitor cocktail	natural abundance

5 3D 1H-13C NOESY

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	CV_2116	[U-100% 13C; U-100% 15N]	0.8 (±0.1) mM
2	TRIS	natural abundance	10 (±0.5) mM
3	sodium chloride	natural abundance	300 (±15.0) mM
4	zinc sulfate	natural abundance	10 (±0.5) uM
5	DTT	natural abundance	10 (±0.5) mM
6	benzamidine	natural abundance	1 (±0.05) mM
7	inhibitor cocktail	natural abundance

6 3D HNCO

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	CV_2116	[U-100% 13C; U-100% 15N]	0.8 (±0.1) mM
2	TRIS	natural abundance	10 (±0.5) mM
3	sodium chloride	natural abundance	300 (±15.0) mM
4	zinc sulfate	natural abundance	10 (±0.5) uM
5	DTT	natural abundance	10 (±0.5) mM
6	benzamidine	natural abundance	1 (±0.05) mM
7	inhibitor cocktail	natural abundance

7 3D HNCA

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	CV_2116	[U-100% 13C; U-100% 15N]	0.8 (±0.1) mM
2	TRIS	natural abundance	10 (±0.5) mM
3	sodium chloride	natural abundance	300 (±15.0) mM
4	zinc sulfate	natural abundance	10 (±0.5) uM
5	DTT	natural abundance	10 (±0.5) mM
6	benzamidine	natural abundance	1 (±0.05) mM
7	inhibitor cocktail	natural abundance

8 3D HNCACB

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	CV_2116	[U-100% 13C; U-100% 15N]	0.8 (±0.1) mM
2	TRIS	natural abundance	10 (±0.5) mM
3	sodium chloride	natural abundance	300 (±15.0) mM
4	zinc sulfate	natural abundance	10 (±0.5) uM
5	DTT	natural abundance	10 (±0.5) mM
6	benzamidine	natural abundance	1 (±0.05) mM
7	inhibitor cocktail	natural abundance

9 3D CBCA(CO)NH

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	CV_2116	[U-100% 13C; U-100% 15N]	0.8 (±0.1) mM
2	TRIS	natural abundance	10 (±0.5) mM
3	sodium chloride	natural abundance	300 (±15.0) mM
4	zinc sulfate	natural abundance	10 (±0.5) uM
5	DTT	natural abundance	10 (±0.5) mM
6	benzamidine	natural abundance	1 (±0.05) mM
7	inhibitor cocktail	natural abundance

10 3D HN(CO)CA

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	CV_2116	[U-100% 13C; U-100% 15N]	0.8 (±0.1) mM
2	TRIS	natural abundance	10 (±0.5) mM
3	sodium chloride	natural abundance	300 (±15.0) mM
4	zinc sulfate	natural abundance	10 (±0.5) uM
5	DTT	natural abundance	10 (±0.5) mM
6	benzamidine	natural abundance	1 (±0.05) mM
7	inhibitor cocktail	natural abundance

11 3D HBHA(CO)NH

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	CV_2116	[U-100% 13C; U-100% 15N]	0.8 (±0.1) mM
2	TRIS	natural abundance	10 (±0.5) mM
3	sodium chloride	natural abundance	300 (±15.0) mM
4	zinc sulfate	natural abundance	10 (±0.5) uM
5	DTT	natural abundance	10 (±0.5) mM
6	benzamidine	natural abundance	1 (±0.05) mM
7	inhibitor cocktail	natural abundance

12 3D C(CO)NH

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	CV_2116	[U-100% 13C; U-100% 15N]	0.8 (±0.1) mM
2	TRIS	natural abundance	10 (±0.5) mM
3	sodium chloride	natural abundance	300 (±15.0) mM
4	zinc sulfate	natural abundance	10 (±0.5) uM
5	DTT	natural abundance	10 (±0.5) mM
6	benzamidine	natural abundance	1 (±0.05) mM
7	inhibitor cocktail	natural abundance

13 3D HCCH-TOCSY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	CV_2116	[U-100% 13C; U-100% 15N]	0.8 (±0.1) mM
2	TRIS	natural abundance	10 (±0.5) mM
3	sodium chloride	natural abundance	300 (±15.0) mM
4	zinc sulfate	natural abundance	10 (±0.5) uM
5	DTT	natural abundance	10 (±0.5) mM
6	benzamidine	natural abundance	1 (±0.05) mM
7	inhibitor cocktail	natural abundance

14 3D HCCH-COSY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	CV_2116	[U-100% 13C; U-100% 15N]	0.8 (±0.1) mM
2	TRIS	natural abundance	10 (±0.5) mM
3	sodium chloride	natural abundance	300 (±15.0) mM
4	zinc sulfate	natural abundance	10 (±0.5) uM
5	DTT	natural abundance	10 (±0.5) mM
6	benzamidine	natural abundance	1 (±0.05) mM
7	inhibitor cocktail	natural abundance

15 3D (H)CCH-TOCSY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
15	CV_2116	[U-100% 13C; U-100% 15N]	0.8 (±0.1) mM
16	TRIS	natural abundance	10 (±0.5) mM
17	sodium chloride	natural abundance	300 (±15.0) mM
18	zinc sulfate	natural abundance	10 (±0.05) uM
19	DTT	natural abundance	10 (±0.5) mM
20	benzamidine	natural abundance	1 (±0.05) mM
21	inhibitor cocktail	natural abundance

16 4D CC NOESY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
15	CV_2116	[U-100% 13C; U-100% 15N]	0.8 (±0.1) mM
16	TRIS	natural abundance	10 (±0.5) mM
17	sodium chloride	natural abundance	300 (±15.0) mM
18	zinc sulfate	natural abundance	10 (±0.05) uM
19	DTT	natural abundance	10 (±0.5) mM
20	benzamidine	natural abundance	1 (±0.05) mM
21	inhibitor cocktail	natural abundance

17 2D His-HMQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	CV_2116	[U-100% 13C; U-100% 15N]	0.8 (±0.1) mM
2	TRIS	natural abundance	10 (±0.5) mM
3	sodium chloride	natural abundance	300 (±15.0) mM
4	zinc sulfate	natural abundance	10 (±0.5) uM
5	DTT	natural abundance	10 (±0.5) mM
6	benzamidine	natural abundance	1 (±0.05) mM
7	inhibitor cocktail	natural abundance

nullKeywords Chromobacterium violaceum, CV_2116, NMR structure, uncharacterized protein

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (0.638 seconds)

BMRB: 16521

Sample #1

Sample #2

Sample #3

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. CV 2116, : 1 entities Detail

Experiments performed 17 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample