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BMRB: 16544

2KMU

RECQL4 AMINO-TERMINAL DOMAIN

Authors

Ohlenschlager, O.H.J., Gorlach, M., Pospiech, H.

Assembly

RECQL4

Entity

1. RECQL4 (polymer, Thiol state: not present), 56 monomers, 6835.524 Da Detail

GSMERLRDVR ERLQAWERAF RRQRGRRPSQ DDVEAAPEET RALYREYRTL KRTTGQ

Formula weight

6835.524 Da

Source organism

Exptl. method

Refine. method

DGSA-distance geometry simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 91.8 %, Completeness (bb): 96.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	91.8 % (619 of 674)	89.8 % (327 of 364)	95.6 % (240 of 251)	88.1 % (52 of 59)
Backbone	96.7 % (321 of 332)	96.5 % (109 of 113)	97.0 % (160 of 165)	96.3 % (52 of 54)
Sidechain	88.6 % (350 of 395)	86.9 % (218 of 251)	95.0 % (132 of 139)	0.0 % (0 of 5)
Aromatic	63.2 % (24 of 38)	63.2 % (12 of 19)	66.7 % (12 of 18)	0.0 % (0 of 1)
Methyl	100.0 % (42 of 42)	100.0 % (21 of 21)	100.0 % (21 of 21)

1. RECQL4NT

GSMERLRDVR ERLQAWERAF RRQRGRRPSQ DDVEAAPEET RALYREYRTL KRTTGQ

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 7.4

#	Name	Isotope labeling	Type	Concentration
1	RECQL4NT	[U-99% 15N]		1.2 mM
2	TRIS	[U-2H]		20 mM
3	sodium chloride	natural abundance		100 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 7.4

#	Name	Isotope labeling	Type	Concentration
4	RECQL4NT	[U-99% 13C; U-99% 15N]		1.0 mM
5	TRIS	[U-2H]		20 mM
6	sodium chloride	natural abundance		100 mM

Protein Blocks Logo

Calculated from 20 models in PDB: 2KMU, Strand ID: A Detail

Release date

2012-08-02

Citation

The N-terminus of the human RecQL4 helicase is a homeodomain-like DNA interaction motif
Ohlenschlager, O., Kuhnert, A., Schneider, A., Haumann, S., Bellstedt, P., Keller, H., Saluz, H., Hortschansky, P., Hanel, F., Grosse, F., Gorlach, M., Pospiech, H.
Nucleic Acids Res. (2012), 40, 8309-8324, PubMed 22730300 , DOI 10.1093/nar/gks591 , Abstract

Related entities 1. RECQL4, : 1 : 2 : 15 entities Detail

Interaction partners 1. RECQL4, : 10 interactors Detail

More details for 10 interactors identified by 6 citations

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 7.4

#	Name	Isotope labeling	Type	Concentration
1	RECQL4NT	[U-99% 15N]		1.2 mM
2	TRIS	[U-2H]		20 mM
3	sodium chloride	natural abundance		100 mM

2 2D 1H-13C HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 7.4

#	Name	Isotope labeling	Type	Concentration
4	RECQL4NT	[U-99% 13C; U-99% 15N]		1.0 mM
5	TRIS	[U-2H]		20 mM
6	sodium chloride	natural abundance		100 mM

3 3D HNCACB

Instrument

Bruker Avance - 750 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 7.4

#	Name	Isotope labeling	Type	Concentration
4	RECQL4NT	[U-99% 13C; U-99% 15N]		1.0 mM
5	TRIS	[U-2H]		20 mM
6	sodium chloride	natural abundance		100 mM

4 3D H(CCO)NH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 7.4

#	Name	Isotope labeling	Type	Concentration
4	RECQL4NT	[U-99% 13C; U-99% 15N]		1.0 mM
5	TRIS	[U-2H]		20 mM
6	sodium chloride	natural abundance		100 mM

5 3D HNHA

Instrument

Bruker Avance - 750 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 7.4

#	Name	Isotope labeling	Type	Concentration
1	RECQL4NT	[U-99% 15N]		1.2 mM
2	TRIS	[U-2H]		20 mM
3	sodium chloride	natural abundance		100 mM

6 3D HCCH-TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 7.4

#	Name	Isotope labeling	Type	Concentration
4	RECQL4NT	[U-99% 13C; U-99% 15N]		1.0 mM
5	TRIS	[U-2H]		20 mM
6	sodium chloride	natural abundance		100 mM

7 3D HCCH-COSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 7.4

#	Name	Isotope labeling	Type	Concentration
4	RECQL4NT	[U-99% 13C; U-99% 15N]		1.0 mM
5	TRIS	[U-2H]		20 mM
6	sodium chloride	natural abundance		100 mM

8 3D 1H-15N NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 7.4

#	Name	Isotope labeling	Type	Concentration
1	RECQL4NT	[U-99% 15N]		1.2 mM
2	TRIS	[U-2H]		20 mM
3	sodium chloride	natural abundance		100 mM

9 3D 1H-13C NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 7.4

#	Name	Isotope labeling	Type	Concentration
4	RECQL4NT	[U-99% 13C; U-99% 15N]		1.0 mM
5	TRIS	[U-2H]		20 mM
6	sodium chloride	natural abundance		100 mM

nullKeywords DNA-REPLICATION, HELICASE, HOMEODOMAIN-LIKE, RECQL4