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BMRB: 16545

2KP6

Solution NMR structure of protein CV0237 from Chromobacterium violaceum. Northeast Structural Genomics Consortium (NESG) target CvT1.

Authors

Fares, C., Lemak, A., Yee, A., Garcia, M., Arrowsmith, C.T.

Assembly

CV0237

Entity

1. CV0237 (polymer, Thiol state: all free), 82 monomers, 8940.949 Da Detail

QGHMDTSNHL LPGLFRQLGL EDEPAAIRAF IDSHPLPPRV PLPEAPFWTP AQAAFLRQAL ECDAEWSEAA DGLAVLLQQG EA

Formula weight

8940.949 Da

Source organism

Chromobacterium violaceum

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 96.3 %, Completeness: 84.7 %, Completeness (bb): 90.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	84.7 % (788 of 930)	84.0 % (405 of 482)	86.6 % (317 of 366)	80.5 % (66 of 82)
Backbone	90.1 % (427 of 474)	89.4 % (143 of 160)	91.3 % (220 of 241)	87.7 % (64 of 73)
Sidechain	81.8 % (436 of 533)	81.4 % (262 of 322)	85.1 % (172 of 202)	22.2 % (2 of 9)
Aromatic	34.2 % (26 of 76)	34.2 % (13 of 38)	30.6 % (11 of 36)	100.0 % (2 of 2)
Methyl	100.0 % (94 of 94)	100.0 % (47 of 47)	100.0 % (47 of 47)

1. CV0237

QGHMDTSNHL LPGLFRQLGL EDEPAAIRAF IDSHPLPPRV PLPEAPFWTP AQAAFLRQAL ECDAEWSEAA DGLAVLLQQG EA

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	CV0237	[U-99% 13C; U-99% 15N]	0.4 ~ 0.7 mM
2	TRIS	[U-99% 2H]	10 mM
3	sodium chloride	natural abundance	300 mM
4	DTT	[U-99% 2H]	10 mM
5	sodium azide	natural abundance	0.01 %
6	Zinc Sulfate	natural abundance	10 uM
7	Roche inhibitor cocktail	natural abundance	1 na
8	H20	natural abundance	90 %
9	D20	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
¹H	DSS	methyl protons	-0.07 ppm	external	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

Referencing offset: -0.27 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
¹H	DSS	methyl protons	-0.07 ppm	external	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

Referencing offset: -0.27 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
¹H	DSS	methyl protons	-0.07 ppm	external	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

Referencing offset: 0.15 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
¹H	DSS	methyl protons	-0.07 ppm	external	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

Referencing offset: -0.17 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2KP6, Strand ID: A Detail

Release date

2010-11-09

Citation

Solution structure of protein CV0237 from Chromobacterium violaceum
Fares, C., Lemak, A., Yee, A., Garcia, M., Arrowsmith, C.

Related entities 1. CV0237, : 1 : 1 entities Detail

Experiments performed 15 experiments Detail

1 2D 1H-15N HSQC