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BMRB: 16549

2KP7

Solution NMR structure of the Mus81 N-terminal HhH. Northeast Structural Genomics Consortium target MmT1A

Authors

Laister, R.C., Wu, B., Lemak, A., Arrowsmith, C.H.

Assembly

Mus81 NTD

Entity

1. Mus81 NTD (polymer, Thiol state: all free), 80 monomers, 9379.780 Da Detail

RPLPVCPNPP FVRWLTEWRD EAASRGRHTR FVFQKALRSL QRYPLPLRSG KEAKILQHFG DRLCRMLDEK LKQHLASGGD

Formula weight

9379.78 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 97.5 %, Completeness: 80.4 %, Completeness (bb): 79.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	80.4 % (793 of 986)	86.7 % (456 of 526)	69.5 % (264 of 380)	91.3 % (73 of 80)
Backbone	79.6 % (371 of 466)	93.7 % (148 of 158)	66.0 % (155 of 235)	93.2 % (68 of 73)
Sidechain	82.7 % (492 of 595)	83.7 % (308 of 368)	81.4 % (179 of 220)	71.4 % (5 of 7)
Aromatic	47.6 % (40 of 84)	54.8 % (23 of 42)	37.5 % (15 of 40)	100.0 % (2 of 2)
Methyl	89.2 % (66 of 74)	89.2 % (33 of 37)	89.2 % (33 of 37)

1. Mus81 NTD

RPLPVCPNPP FVRWLTEWRD EAASRGRHTR FVFQKALRSL QRYPLPLRSG KEAKILQHFG DRLCRMLDEK LKQHLASGGD

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	Mus81 NTD	[U-100% 13C; U-100% 15N]	1.0 mM
2	sodium phosphate	natural abundance	25 mM
3	sodium chloride	natural abundance	500 mM
4	DTT	natural abundance	2 mM
5	benzamidine	natural abundance	2 mM
6	PMSF	natural abundance	0.5 mM
7	EDTA	natural abundance	0.5 mM
8	TCEP	natural abundance	0.5 mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
9	Mus81 NTD	[U-100% 13C; U-100% 15N]	1 mM
10	sodium phosphate	natural abundance	25 mM
11	sodium chloride	natural abundance	500 mM
12	benzamidine	natural abundance	2 mM
13	DTT	natural abundance	2 mM
14	EDTA	natural abundance	0.5 mM
15	PMSF	natural abundance	0.5 mM
16	TCEP	natural abundance	0.5 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 2.46 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 2.46 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.06 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2KP7, Strand ID: A Detail

Release date

2009-11-08

Citation

Solution NMR structure and DNA binding mode of the Mus81 n-terminal helix-hairpin-helix domain
Laister, R.C., Duan, S., Wu, B., Herzanych, N., Arrowsmith, C.H.
Not known

Related entities 1. Mus81 NTD, : 1 : 2 : 25 entities Detail

Interaction partners 1. Mus81 NTD, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 13 experiments Detail

1 3D CBCA(CO)NH