BMRBj - BMREntryMaster

	Total	¹H
All	75.1 % (136 of 181)	75.1 % (136 of 181)
Backbone	92.0 % (46 of 50)	92.0 % (46 of 50)
Sidechain	68.7 % (90 of 131)	68.7 % (90 of 131)
Aromatic	55.6 % (5 of 9)	55.6 % (5 of 9)
Methyl	91.7 % (11 of 12)	91.7 % (11 of 12)

1. ARNO(375-400)

VSVDPFYEML AARKKRISVK KKQEQP

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Temperature 298 K, pH 7.0, Details 1 mM

#	Name	Isotope labeling	Type	Concentration
1	ARNO(375-400)	[U-100% 15N]		1-2 mM

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Calculated from 12 models in PDB: 2KPA, Strand ID: A Detail

Release date

2010-02-17

Citation

Specific motifs of the V-ATPase a2-subunit isoform interact with catalytic and regulatory domains of ARNO
Merkulova, M., Bakulina, A., Thaker, Y., Gruber, G., Marshansky, V.
Biochim. Biophys. Acta (2010), 1797, 1398-1409, PubMed 20153292 , DOI 10.1016/j.bbabio.2010.02.009 , Abstract

Related entities 1. ARNO(375-400), : 1 : 9 : 1 : 21 entities Detail

Interaction partners 1. ARNO(375-400), : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 2 experiments Detail

NMR combined restraints 5 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints	combined_16550_2kpa.nef
Input source #2: Coordindates	2kpa.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

--------10--------20------
VSVDPFYEMLAARKKRISVKKKQEQP
||||||||||||||||||||||||||
VSVDPFYEMLAARKKRISVKKKQEQP

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	26	0	0	100.0

Content subtype: combined_16550_2kpa.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	136		96.2 (chain: A, length: 26)
1	Distance restraints	DYANA/DIANA_distance_constraints_2	Warning	434 (1)	noe	96.2 (chain: A, length: 26)
1	Dihedral angle restraints	DYANA/DIANA_dihedral_4	OK	129 (129)	.	96.2 (chain: A, length: 26)
2	Dihedral angle restraints	DYANA/DIANA_dihedral_5	OK	144 (144)	.	84.6 (chain: A, length: 26)

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 26, Sequence coverage: 96.2%
- Total number of assignments
  - ¹H chemical shifts: 136
- Completeness of assignments
  - Entity_assemble_ID: A
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
13	ARG	HH11	7.181
16	ARG	HH11	7.305

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	181	134	74.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	50	46	92.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	131	88	67.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	13	10	76.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	9	5	55.6

Distance restraints

Saveframe: DYANA/DIANA_distance_constraints_2
- Status: Warning
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 26, Sequence coverage: 96.2%
- Total number of restraints: 434
- Weight of restraints: 1.0 (434)
- Potential type of restraints: upper-bound-parabolic (434)
- Range of distance target values: 2.66, 5.5 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
- Distance restraints on contact map

Dihedral angle restraints

Saveframe: DYANA/DIANA_dihedral_4
- Status: OK
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 26, Sequence coverage: 96.2%
- Total number of restraints: 129
- Total number of restraints per polymer type: 129
- Weight of restraints: 1.0 (129)
- Potential type of restraints: square-well-parabolic (129)
- Plot of Phi-Psi angle restraints
- Dihedral angle restraints per residue
  - Chain_ID: A
- Saveframe: DYANA/DIANA_dihedral_5
  - Status: OK
  - Experiment type: .
  - Sequence coverage of experimental data
    - Entity_assembly_ID: A, Chain length: 26, Sequence coverage: 84.6%
  - Total number of restraints: 144
  - Total number of restraints per polymer type: 144
  - Weight of restraints: 1.0 (144)
  - Potential type of restraints: square-well-parabolic (144)
  - Plot of Chi1-Chi2 angle restraints
  - Dihedral angle restraints per residue
    - Chain_ID: A

Keywords Peptide

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Found 1 Document (0.57 seconds)

BMRB: 16550

Sample

Related entities 1. ARNO(375-400), : 1 : 9 : 1 : 21 entities Detail

Interaction partners 1. ARNO(375-400), : 1 interactors Detail

Experiments performed 2 experiments Detail

Instrument

Sample

Instrument

Sample

NMR combined restraints 5 contents Detail