BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	95.8 % (1400 of 1462)	95.5 % (722 of 756)	95.9 % (557 of 581)	96.8 % (121 of 125)
Backbone	96.3 % (726 of 754)	96.2 % (251 of 261)	96.3 % (360 of 374)	96.6 % (115 of 119)
Sidechain	95.8 % (789 of 824)	95.2 % (471 of 495)	96.6 % (312 of 323)	100.0 % (6 of 6)
Aromatic	96.0 % (96 of 100)	96.0 % (48 of 50)	96.0 % (48 of 50)
Methyl	97.4 % (148 of 152)	97.4 % (74 of 76)	97.4 % (74 of 76)

1. MAGI-1 PDZ1

GAMGKPFFTR NPSELKGKFI HTKLRKSSRG FGFTVVGGDE PDEFLQIKSL VLDGPAALDG KMETGDVIVS VNDTCVLGHT HAQVVKIFQS IPIGASVDLE LCRGYPLPFD PDDPNTSLVT SVAILDKEP

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 295 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	MAGI-1 PDZ1	natural abundance	0.2 ~ 0.6 mM
2	sodium phosphate	natural abundance	0.02 ~ 0.1 mM
3	sodium chloride	natural abundance	50 mM
4	DTT	natural abundance	2 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 295 K, pH 6.8

#	Name	Isotope labeling	Concentration
7	MAGI-1 PDZ1	[U-15N]	0.2 ~ 0.6 mM
8	sodium phosphate	natural abundance	0.02 ~ 0.1 mM
9	sodium chloride	natural abundance	50 mM
10	DTT	natural abundance	2 mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 295 K, pH 6.8

#	Name	Isotope labeling	Concentration
13	MAGI-1 PDZ1	[U-100% 13C; U-100% 15N]	0.2 ~ 0.6 mM
14	sodium phosphate	natural abundance	0.02 ~ 0.1 mM
15	sodium chloride	natural abundance	50 mM
16	DTT	natural abundance	2 mM
17	H2O	natural abundance	90 %
18	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.2 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2KPK, Strand ID: A Detail

Release date

2012-05-08

Citation 1

13C, 15N and 1H resonance assignment of the PDZ1 domain of MAGI-1 using QUASI
Charbonier, S., Coutouly, M., Kieffer, B., Trave, G., Atkinson, R.A.
J. Biomol. NMR (2006), 36 Suppl 1, 33-33, PubMed 16636753 , DOI 10.1007/s10858-006-0017-7 , Abstract

Citation 2

The structural and dynamic response of MAGI-1 PDZ1 with noncanonical domain boundaries to the binding of human papillomavirus E6
Charbonier, S., Nomine, Y., Ramirez, J., Luck, K., Chapelle, A., Stote, R.H., Trave, G., Kieffer, B., Atkinson, R.A.
J. Mol. Biol. (2011), 406, 745-763, PubMed 21238461 , DOI 10.1016/j.jmb.2011.01.015 , Abstract

1 3D HN(CO)CA