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BMRB: 16569

2KPT

Solution NMR structure of the N-terminal domain of cg2496 protein from Corynebacterium glutamicum. Northeast Structural Genomics Consortium Target CgR26A

Authors

Eletsky, A., Sathyamoorthy, B., Sukumaran, D.K., Wang, D., Buchwald, W.A., Ciccosanti, C., Janjua, H., Nair, R., Rost, B., Acton, T.B., Xiao, R., Everett, J.K., Montelione, G.T., Szyperski, T.

Assembly

cgr26a

Entity

1. cgr26a (polymer, Thiol state: not present), 148 monomers, 15822.69 Da Detail

TETYVLAESP EFYQDNVTDY TGQISSSDIT NIQAAIDDVK ASEQKVIFVV FLSSFDGVDP ETWTQQALQA NGGGNVLIYA LAPEERQYGI QGGTQWTDAE LDAANNAAFQ ALSQEDWAGS ALALAESVGS SSSSSSGSSS LEHHHHHH

Formula weight

15822.69 Da

Source organism

Corynebacterium glutamicum

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.9 %, Completeness: 93.2 %, Completeness (bb): 93.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	93.2 % (1483 of 1592)	92.7 % (747 of 806)	93.5 % (580 of 620)	94.0 % (156 of 166)
Backbone	93.1 % (821 of 882)	92.8 % (282 of 304)	93.3 % (404 of 433)	93.1 % (135 of 145)
Sidechain	93.3 % (790 of 847)	92.6 % (465 of 502)	93.8 % (304 of 324)	100.0 % (21 of 21)
Aromatic	82.7 % (124 of 150)	80.0 % (60 of 75)	84.7 % (61 of 72)	100.0 % (3 of 3)
Methyl	100.0 % (158 of 158)	100.0 % (79 of 79)	100.0 % (79 of 79)

1. cgr26a

TETYVLAESP EFYQDNVTDY TGQISSSDIT NIQAAIDDVK ASEQKVIFVV FLSSFDGVDP ETWTQQALQA NGGGNVLIYA LAPEERQYGI QGGTQWTDAE LDAANNAAFQ ALSQEDWAGS ALALAESVGS SSSSSSGSSS LEHHHHHH

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	cgr26a	[U-100% 13C; U-100% 15N]	0.9 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	200 mM
4	calcium chloride	natural abundance	5 mM
5	DSS	natural abundance	50 uM
6	sodium azide	natural abundance	0.02 %
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
9	cgr26a	[U-100% 13C; U-100% 15N]	0.9 mM
10	MES	natural abundance	20 mM
11	sodium chloride	natural abundance	200 mM
12	calcium chloride	natural abundance	5 mM
13	DSS	natural abundance	50 uM
14	sodium azide	natural abundance	0.02 %
15	H2O	natural abundance	90 %
16	D2O	natural abundance	10 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
17	cgr26a	[U-5% 13C; U-100% 15N]	0.9 mM
18	MES	natural abundance	20 mM
19	sodium chloride	natural abundance	200 mM
20	calcium chloride	natural abundance	5 mM
21	DSS	natural abundance	50 uM
22	sodium azide	natural abundance	0.02 %
23	H2O	natural abundance	90 %
24	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.13 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.13 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.08 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.06 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2KPT, Strand ID: A Detail

Release date

2010-11-23

Citation

Solution NMR structures reveal a distinct architecture and provide first structures for protein domain family PF04536
Eletsky, A., Sathyamoorthy, B., Sukumaran, D.K., Wang, D., Buchwald, W., Ciccosanti, C., Janjua, H., Nair, R., Rost, B., Acton, T.B., Xiao, R., Everett, J.K., Montelione, G.T., Szyperski, T.
J. Struct. Funct. Genomics (2012), 13, 9-14, PubMed 22198206 , DOI 10.1007/s10969-011-9122-2 , Abstract

Related entities 1. cgr26a, : 1 entities Detail

Experiments performed 18 experiments Detail

1 2D 1H-15N HSQC