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BMRB: 16572

2KPW

NMR solution structure of Lamin-B1 protein from Home sapiens: Northeast Structural Genomics Consortium target, HR5546A(438-548)

Authors

Swapna, G., Montelione, G.T.

Assembly

Lamin-B1

Entity

1. Lamin-B1 (polymer, Thiol state: not present), 122 monomers, 13652.14 Da Detail

MGHHHHHHSH MTGNVCIEEI DVDGKFIRLK NTSEQDQPMG GWEMIRKIGD TSVSYKYTSR YVLKAGQTVT IWAANAGVTA SPPTDLIWKN QNSWGTGEDV KVILKNSQGE EVAQRSTVFK TT

Formula weight

13652.14 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.4 %, Completeness: 86.9 %, Completeness (bb): 82.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	86.9 % (1215 of 1398)	93.6 % (676 of 722)	75.0 % (406 of 541)	98.5 % (133 of 135)
Backbone	82.2 % (597 of 726)	95.2 % (240 of 252)	67.6 % (240 of 355)	98.3 % (117 of 119)
Sidechain	93.0 % (728 of 783)	93.0 % (437 of 470)	92.6 % (275 of 297)	100.0 % (16 of 16)
Aromatic	74.2 % (89 of 120)	75.0 % (45 of 60)	71.4 % (40 of 56)	100.0 % (4 of 4)
Methyl	100.0 % (124 of 124)	100.0 % (62 of 62)	100.0 % (62 of 62)

1. Lamin-B1 protein

MGHHHHHHSH MTGNVCIEEI DVDGKFIRLK NTSEQDQPMG GWEMIRKIGD TSVSYKYTSR YVLKAGQTVT IWAANAGVTA SPPTDLIWKN QNSWGTGEDV KVILKNSQGE EVAQRSTVFK TT

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 4.5, Details 1.219mM Protein, 20mM NH4OAc, 200 mM NaCl, 5mM CaCl2, 10 mM DTT, 50 uM DSS

#	Name	Isotope labeling	Concentration
1	Lamin-B1 protein	[U-100% 13C; U-100% 15N]	1.219 (±0.2) mM
2	NH4OAc	natural abundance	20 mM
3	DTT	natural abundance	100 mM
4	NaCl	natural abundance	200 mM
5	CaCl2	natural abundance	5 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 4.5, Details 1.219mM Protein, 20mM NH4OAc, 200 mM NaCl, 5mM CaCl2, 10 mM DTT, 50 uM DSS

#	Name	Isotope labeling	Concentration
6	Lamin-B1 protein	[U-5% 13C; U-100% 15N]	1.219 (±0.2) mM
7	NH4OAc	natural abundance	20 mM
8	DTT	natural abundance	100 mM
9	NaCl	natural abundance	200 mM
10	CaCl2	natural abundance	5 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.2 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.2 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2KPW, Strand ID: A Detail

Release date

2009-10-21

Related entities 1. Lamin-B1, : 1 : 75 entities Detail

Interaction partners 1. Lamin-B1, : 75 interactors Detail

More details for 75 interactors identified by 27 citations

Experiments performed 19 experiments Detail

1 2D 1H-15N HSQC