BMRB: 16575

HUMAN NEDD4 3RD WW DOMAIN COMPLEX WITH EBOLA ZAIRE VIRUS MATRIX PROTEIN VP40 DERIVED PEPTIDE ILPTAPPEYMEA

Authors

Iglesias-Bexiga, M., Luque, I., Macias, M.J., Blanco, F.J., Cobos, E.S., Bonet, R.

Assembly

NEDD4 3RD WW/ILPTAPPEYMEA complex

Entity

1. NEDD4-WW3 (polymer, Thiol state: not present), 49 monomers, 5568.197 Da Detail

GAMGPSEIEQ GFLPKGWEVR HAPNGRPFFI DHNTKTTTWE DPRLKIPAH

2. ILPTAPPEYMEA (polymer, Thiol state: not present), 12 monomers, 1331.531 Da Detail

ILPTAPPEYM EA

Total weight

6899.7275 Da

Max. entity weight

5568.197 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

DGSA-distance geometry simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.8 %, Completeness: 89.4 %, Completeness (bb): 85.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	89.4 % (390 of 436)	91.3 % (346 of 379)	77.2 % (44 of 57)
Backbone	85.3 % (145 of 170)	89.8 % (106 of 118)	75.0 % (39 of 52)
Sidechain	92.1 % (245 of 266)	92.0 % (240 of 261)	100.0 % (5 of 5)
Aromatic	92.3 % (36 of 39)	91.9 % (34 of 37)	100.0 % (2 of 2)
Methyl	92.3 % (24 of 26)	92.3 % (24 of 26)

1. NEDD4-WW3

GAMGPSEIEQ GFLPKGWEVR HAPNGRPFFI DHNTKTTTWE DPRLKIPAH

2. ILPTAPPEYMEA

ILPTAPPEYM EA

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 288 K, pH 7.00

#	Name	Isotope labeling	Type	Concentration
1	PROTEIN	[U-100% 15N]		1 mM
2	PEPTIDE	natural abundance		10 mM
3	sodium phosphate	natural abundance		20 mM
4	sodium azide	natural abundance		0.02 v/v
5	H2O	natural abundance		90 %
6	D2O	natural abundance		10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 288 K, pH 7.00

#	Name	Isotope labeling	Type	Concentration
7	PROTEIN	[U-100% 13C; U-100% 15N]		0.5 mM
8	PEPTIDE	natural abundance		5 mM
9	sodium phosphate	natural abundance		20 mM
10	sodium azide	natural abundance		0.02 v/v
11	H2O	natural abundance		90 %
12	D2O	natural abundance		10 %

Hide sample codition

Protein Blocks Logo

Calculated from 20 models in PDB: 2KQ0, Strand ID: A, B Detail

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Release date

2015-06-18

Citation

HUMAN NEDD4 3RD WW DOMAIN COMPLEX WITH EBOLA ZAIRE VIRUS MATRIX PROTEIN VP40 DERIVED PEPTIDE
Iglesias-Bexiga, M.
Not known

Related entities 1. NEDD4-WW3, : 2 : 86 entities Detail

Related entities 2. ILPTAPPEYMEA, : 1 : 2 entities Detail

Interaction partners 1. NEDD4-WW3, : 45 interactors Detail

Experiments performed 3 experiments Detail