BMRB: 16589

peptide_ptha

Authors

Neves, J., Sforca, M., Murakami, M., Zeri, A., Benedetti, C.

Assembly

PthA

Entity

1. PthA (polymer, Thiol state: not present), 51 monomers, 5288.981 Da Detail

EQVVAIASNI GGKQALETVQ RLLPVLCQAH GLTPEQVVAI ASHDGGKQAL E

Formula weight

5288.981 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Refine. method

distance geometry

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 79.4 %, Completeness (bb): 94.3 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H
All	79.4 % (227 of 286)	79.4 % (227 of 286)
Backbone	94.3 % (99 of 105)	94.3 % (99 of 105)
Sidechain	70.7 % (128 of 181)	70.7 % (128 of 181)
Aromatic	50.0 % (2 of 4)	50.0 % (2 of 4)
Methyl	87.2 % (34 of 39)	87.2 % (34 of 39)

1. pthA

EQVVAIASNI GGKQALETVQ RLLPVLCQAH GLTPEQVVAI ASHDGGKQAL E

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 5

#	Name	Isotope labeling	Type	Concentration
1	entity	natural abundance		1.0 mM
2	sodium phosphate	natural abundance		20 mM
3	SDS	natural abundance		70 mM
4	DTT	natural abundance		1 mM
5	H2O	natural abundance		95 %
6	D2O	natural abundance		5 %

Hide sample codition

Protein Blocks Logo

Calculated from 21 models in PDB: 2KQ5, Strand ID: A Detail

---

Release date

2011-05-18

Citation

The repeat domain of the type III effector protein PthA shows a TPR-like structure and undergoes conformational changes upon DNA interaction
Murakami, M., Sforca, M., Neves, J., Paiva, J., Domingues, M., Pereira, A., Zeri, A., Benedetti, C.
Proteins (2010), 78, 3386-3395, PubMed 20848643 , DOI 10.1002/prot.22846 , Abstract

Related entities 1. PthA, : 1 : 29 : 471 entities Detail

Experiments performed 3 experiments Detail