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BMRB: 16596

2KQB

First PBZ domain of human APLF protein

Authors

Neuhaus, D., Eustermann, S., Brockmann, C., Yang, J.

Assembly

APLF

Entity

1. APLF 363-451 (polymer, Thiol state: all other bound), 89 monomers, 10036.93 Da Detail

GPLGSGSEGN KVKRTSCMYG ANCYRKNPVH FQHFSHPGDS DYGGVQIVGQ DETDDRPECP YGPSCYRKNP QHKIEYRHNT LPVRNVLDE

2. ZN (non-polymer), 65.409 × 2 Da

Total weight

10167.748 Da

Max. entity weight

10036.93 Da

Entity Connection

na 8 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS17:SG	2:ZN1:ZN
2	na	sing	1:CYS23:SG	2:ZN1:ZN
3	na	sing	1:HIS30:NE2	2:ZN1:ZN
4	na	sing	1:HIS36:NE2	2:ZN1:ZN
5	na	sing	1:CYS59:SG	2:ZN1:ZN
6	na	sing	1:CYS65:SG	2:ZN1:ZN
7	na	sing	1:HIS72:NE2	2:ZN1:ZN
8	na	sing	1:HIS78:NE2	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.6 %, Completeness: 87.2 %, Completeness (bb): 80.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	87.2 % (882 of 1011)	93.5 % (500 of 535)	76.6 % (295 of 385)	95.6 % (87 of 91)
Backbone	80.5 % (417 of 518)	93.9 % (169 of 180)	66.1 % (170 of 257)	96.3 % (78 of 81)
Sidechain	94.6 % (541 of 572)	93.2 % (331 of 355)	97.1 % (201 of 207)	90.0 % (9 of 10)
Aromatic	100.0 % (88 of 88)	100.0 % (44 of 44)	100.0 % (44 of 44)
Methyl	100.0 % (52 of 52)	100.0 % (26 of 26)	100.0 % (26 of 26)

1. APLF 363-451

GPLGSGSEGN KVKRTSCMYG ANCYRKNPVH FQHFSHPGDS DYGGVQIVGQ DETDDRPECP YGPSCYRKNP QHKIEYRHNT LPVRNVLDE

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 300 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	potassium pyrophosphate	natural abundance	20 mM
2	sodium chloride	natural abundance	200 mM
3	zinc sulphate	natural abundance	100 uM
4	DTT	[U-2H]	2 mM
5	APLF_363-451	[U-98% 13C; U-98% 15N]	0.5-0.6 mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 300 K, pH 6.0

#	Name	Isotope labeling	Concentration
6	potassium pyrophosphate	natural abundance	20 mM
7	sodium chloride	natural abundance	200 mM
8	zinc sulphate	natural abundance	100 uM
9	DTT	[U-2H]	2 mM
10	APLF_363-451	[U-98% 13C; U-98% 15N]	0.5-0.6 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	external	indirect	0.2514495
¹H	TSP	methyl protons	external	direct	1.0
¹⁵N	TSP	methyl protons	external	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 4 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	external	indirect	0.2514495
¹H	TSP	methyl protons	external	direct	1.0
¹⁵N	TSP	methyl protons	external	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 4 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	external	indirect	0.2514495
¹H	TSP	methyl protons	external	direct	1.0
¹⁵N	TSP	methyl protons	external	indirect	0.1013291

Referencing offset: -0.04 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 25 models in PDB: 2KQB, Strand ID: A Detail

Release date

2010-01-27

Citation

Solution structures of the two PBZ domains from human APLF and their interaction with poly(ADP-ribose)
Eustermann, S., Brockmann, C., Mehrotra, P., Yang, J., Loakes, D., West, S.C., Ahel, I., Neuhaus, D.
Nat. Struct. Mol. Biol. (2010), 17, 241-243, PubMed 20098424 , DOI 10.1038/nsmb.1747 , Abstract

Related entities 1. APLF 363-451, : 1 : 3 : 3 : 4 entities Detail

Interaction partners 1. APLF 363-451, : 9 interactors Detail

More details for 9 interactors identified by 5 citations

Experiments performed 17 experiments Detail

1 2D 1H-15N HSQC