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BMRB: 16640

2KRK

Solution NMR Structure of 26S protease regulatory subunit 8 from H.sapiens, Northeast Structural Genomics Consortium Target Target HR3102A

Authors

Liu, G., Janjua, J., Xiao, R., Ciccosanti, C., Shastry, R., Everett, J.K., Nair, R., Acton, T.B., Rost, B., Montelione, G.T.

Assembly

HR3102A

Entity

1. HR3102A (polymer, Thiol state: all free), 86 monomers, 9775.144 Da Detail

MGHHHHHHSH PNEEARLDIL KIHSRKMNLT RGINLRKIAE LMPGASGAEV KGVCTEAGMY ALRERRVHVT QEDFEMAVAK VMQKDS

Formula weight

9775.144 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing, molecular dynamics, and torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.0 %, Completeness: 81.3 %, Completeness (bb): 76.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	81.3 % (804 of 989)	87.1 % (451 of 518)	70.9 % (271 of 382)	92.1 % (82 of 89)
Backbone	76.8 % (393 of 512)	87.5 % (154 of 176)	64.3 % (162 of 252)	91.7 % (77 of 84)
Sidechain	86.5 % (482 of 557)	86.8 % (297 of 342)	85.7 % (180 of 210)	100.0 % (5 of 5)
Aromatic	40.7 % (22 of 54)	48.1 % (13 of 27)	33.3 % (9 of 27)
Methyl	98.8 % (85 of 86)	97.7 % (42 of 43)	100.0 % (43 of 43)

1. HR3102A

MGHHHHHHSH PNEEARLDIL KIHSRKMNLT RGINLRKIAE LMPGASGAEV KGVCTEAGMY ALRERRVHVT QEDFEMAVAK VMQKDS

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 Not defined, Temperature 298 K, pH 6.5, Details 0.82 mM [U-100% 13C; U-100% 15N] SR232, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	HR3102A	[U-100% 13C; U-100% 15N]	0.82 mM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 Not defined, Temperature 298 K, pH 6.5, Details 0.52 mM [10% 13C; U-100% 15N] SR232, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
4	HR3102A	[U-10% 13C; U-100% 15N]	0.52 mM
5	H2O	natural abundance	95 %
6	D2O	natural abundance	5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291
³¹P	DSS	methyl protons	na	indirect	0.4048086

Referencing offset: 0.24 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291
³¹P	DSS	methyl protons	na	indirect	0.4048086

Referencing offset: 0.24 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291
³¹P	DSS	methyl protons	na	indirect	0.4048086

Referencing offset: 0.04 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2KRK, Strand ID: A Detail

Release date

2012-08-02

Related entities 1. HR3102A, : 1 : 35 : 260 entities Detail

Interaction partners 1. HR3102A, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 Not defined, Temperature 298 K, pH 6.5, Details 0.82 mM [U-100% 13C; U-100% 15N] SR232, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	HR3102A	[U-100% 13C; U-100% 15N]	0.82 mM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %

2 2D 1H-13C HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 Not defined, Temperature 298 K, pH 6.5, Details 0.82 mM [U-100% 13C; U-100% 15N] SR232, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	HR3102A	[U-100% 13C; U-100% 15N]	0.82 mM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %

3 3D HNCO

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 Not defined, Temperature 298 K, pH 6.5, Details 0.82 mM [U-100% 13C; U-100% 15N] SR232, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	HR3102A	[U-100% 13C; U-100% 15N]	0.82 mM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %

4 3D CBCA(CO)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 Not defined, Temperature 298 K, pH 6.5, Details 0.82 mM [U-100% 13C; U-100% 15N] SR232, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	HR3102A	[U-100% 13C; U-100% 15N]	0.82 mM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %

5 3D HNCACB

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 Not defined, Temperature 298 K, pH 6.5, Details 0.82 mM [U-100% 13C; U-100% 15N] SR232, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	HR3102A	[U-100% 13C; U-100% 15N]	0.82 mM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %

6 3D 1H-13C arom NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 Not defined, Temperature 298 K, pH 6.5, Details 0.82 mM [U-100% 13C; U-100% 15N] SR232, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	HR3102A	[U-100% 13C; U-100% 15N]	0.82 mM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %

7 3D simutaneous 13C-aromatic,13C-aliphatic,15N edited 1H-1H NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 Not defined, Temperature 298 K, pH 6.5, Details 0.82 mM [U-100% 13C; U-100% 15N] SR232, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	HR3102A	[U-100% 13C; U-100% 15N]	0.82 mM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %

8 2D 1H-13C HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 Not defined, Temperature 298 K, pH 6.5, Details 0.52 mM [10% 13C; U-100% 15N] SR232, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
4	HR3102A	[U-10% 13C; U-100% 15N]	0.52 mM
5	H2O	natural abundance	95 %
6	D2O	natural abundance	5 %

9 GFT HABCABcoNHN

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 Not defined, Temperature 298 K, pH 6.5, Details 0.82 mM [U-100% 13C; U-100% 15N] SR232, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	HR3102A	[U-100% 13C; U-100% 15N]	0.82 mM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %

nullKeywords NORTHEAST STRUCTURAL GENOMICS CONSORTIUM (NESG), PSI-2, Protein NMR, Protein Structure Initiative, Structural Genomics, Target HR3102A

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Found 1 Document (0.9 seconds)

BMRB: 16640

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. HR3102A, : 1 : 35 : 260 entities Detail

Interaction partners 1. HR3102A, : 1 interactors Detail

Experiments performed 9 experiments Detail

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