BMRB: 16654

Solution structure of the human mitochondrial tRNAMet ASL containing the 5-formylcytidine modification in position 34

Authors

Bilbille, Y., Agris, P.F.

Assembly

5'-R(*(PSU)P*CP*GP*GP*GP*CP*CP*(N)P*AP*UP*AP*CP*CP*CP*CP*GP*A)-3'

Entity

1. 5'-R(*(PSU)P*CP*GP*GP*GP*CP*CP*(N)P*AP*UP*AP*CP*CP*CP*CP*GP*A)-3' (polymer, Thiol state: not present), 17 monomers, 5331.154 Da Detail

XCGGGCCXAU ACCCCGA

Formula weight

5331.154 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 88.2 %, Completeness: 83.0 %, Completeness (bb): 84.4 % Detail

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Polymer type: polyribonucleotide

	Total	1H
All	83.0 % (112 of 135)	83.0 % (112 of 135)
Suger, PO4	84.4 % (76 of 90)	84.4 % (76 of 90)
Nucleobase	80.0 % (36 of 45)	80.0 % (36 of 45)
Aromatic	90.3 % (28 of 31)	90.3 % (28 of 31)

1. RNA (5'-R(*(PSU)P*CP*GP*GP*GP*CP*CP*(N)P*AP*UP*AP*CP*CP*CP*CP*GP*A)-3')

XCGGGCCXAU ACCCCGA

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	RNA (5'-R(*(PSU)P*CP*GP*GP*GP*CP*CP*(N)P*AP*UP*AP*CP*CP*CP*CP*GP*A)-3')	natural abundance		1.0 mM
2	phosphate buffer	natural abundance		20 mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
3	RNA (5'-R(*(PSU)P*CP*GP*GP*GP*CP*CP*(N)P*AP*UP*AP*CP*CP*CP*CP*GP*A)-3')	natural abundance		1 mM
4	phosphate buffer	natural abundance		20 mM

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Release date

2011-05-31

Citation

The human mitochondrial tRNAMet: structure/function relationship of a unique modification in the decoding of unconventional codons
Bilbille, Y., Gustilo, E.M., Harris, K.A., Jones, C.N., Lusic, H., Kaiser, R.J., Delaney, M.O., Spremulli, L.L., Deiters, A., Agris, P.F.
J. Mol. Biol. (2011), 406, 257-274, PubMed 21168417 , DOI 10.1016/j.jmb.2010.11.042 , Abstract

Related entities 1. 5'-R(*(PSU)P*CP*GP*GP*GP*CP*CP*(N)P*AP*UP*AP*CP*CP*CP*CP*GP*A)-3', : 1 : 2 : 1 entities Detail

Experiments performed 7 experiments Detail