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BMRB: 16655

2KRZ

Solution structure of the Human Mitochondrial tRNAMet

Authors

Bilbille, Y., Agris, P.F.

Assembly

5'-R(*(PSU)P*CP*GP*GP*GP*CP*CP*CP*AP*UP*AP*CP*CP*CP*CP*GP*A)-3'

Entity

1. 5'-R(*(PSU)P*CP*GP*GP*GP*CP*CP*CP*AP*UP*AP*CP*CP*CP*CP*GP*A)-3' (polymer, Thiol state: not present), 17 monomers, 5440.241 Da Detail

XCGGGCCCAU ACCCCGA

Formula weight

5440.241 Da

Source organism

Exptl. method

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.1 %, Completeness: 82.1 %, Completeness (bb): 94.8 % Detail

Polymer type: polyribonucleotide

	Total	¹H
All	82.1 % (119 of 145)	82.1 % (119 of 145)
Suger, PO4	94.8 % (91 of 96)	94.8 % (91 of 96)
Nucleobase	57.1 % (28 of 49)	57.1 % (28 of 49)
Aromatic	84.8 % (28 of 33)	84.8 % (28 of 33)

1. RNA (5'-R(*(PSU)P*CP*GP*GP*GP*CP*CP*CP*AP*UP*AP*CP*CP*CP*CP*GP*A)-3')

XCGGGCCCAU ACCCCGA

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	RNA (5'-R((PSU)PCPGPGPGPCPCPCPAPUPAPCPCPCPCPGP*A)-3')	natural abundance		1.2 mM
2	phosphate buffer	natural abundance		20 mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
3	RNA (5'-R((PSU)PCPGPGPGPCPCPCPAPUPAPCPCPCPCPGP*A)-3')	natural abundance		1.2 mM
4	phosphate buffer	natural abundance		20 mM

Release date

2011-05-31

Citation

The human mitochondrial tRNAMet: structure/function relationship of a unique modification in the decoding of unconventional codons
Bilbille, Y., Gustilo, E.M., Harris, K.A., Jones, C.N., Lusic, H., Kaiser, R.J., Delaney, M.O., Spremulli, L.L., Deiters, A., Agris, P.F.
J. Mol. Biol. (2011), 406, 257-274, PubMed 21168417 , DOI 10.1016/j.jmb.2010.11.042 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 16654 released on 2011-05-31
Title Solution structure of the human mitochondrial tRNAMet ASL containing the 5-formylcytidine modification in position 34

Related entities 1. 5'-R((PSU)PCPGPGPGPCPCPCPAPUPAPCPCPCPCPGP*A)-3', : 1 : 15 entities Detail

Experiments performed 7 experiments Detail

1 2D 1H-1H NOESY

Instrument

Varian INOVA - 600 MHz Cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	RNA (5'-R((PSU)PCPGPGPGPCPCPCPAPUPAPCPCPCPCPGP*A)-3')	natural abundance		1.2 mM
2	phosphate buffer	natural abundance		20 mM

2 2D 1H-1H NOESY

Instrument

Varian INOVA - 600 MHz Cryoprobe

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
3	RNA (5'-R((PSU)PCPGPGPGPCPCPCPAPUPAPCPCPCPCPGP*A)-3')	natural abundance		1.2 mM
4	phosphate buffer	natural abundance		20 mM

3 2D DQF-COSY

Instrument

Varian INOVA - 600 MHz Cryoprobe

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
3	RNA (5'-R((PSU)PCPGPGPGPCPCPCPAPUPAPCPCPCPCPGP*A)-3')	natural abundance		1.2 mM
4	phosphate buffer	natural abundance		20 mM

4 2D 1H-1H COSY

Instrument

Varian INOVA - 600 MHz Cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	RNA (5'-R((PSU)PCPGPGPGPCPCPCPAPUPAPCPCPCPCPGP*A)-3')	natural abundance		1.2 mM
2	phosphate buffer	natural abundance		20 mM

5 2D 1H-1H TOCSY

Instrument

Varian INOVA - 600 MHz Cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	RNA (5'-R((PSU)PCPGPGPGPCPCPCPAPUPAPCPCPCPCPGP*A)-3')	natural abundance		1.2 mM
2	phosphate buffer	natural abundance		20 mM

6 2D 1H-13C HSQC

Instrument

Varian INOVA - 600 MHz Cryoprobe

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
3	RNA (5'-R((PSU)PCPGPGPGPCPCPCPAPUPAPCPCPCPCPGP*A)-3')	natural abundance		1.2 mM
4	phosphate buffer	natural abundance		20 mM

7 2D 1H-31P HETCOR

Instrument

Varian INOVA - 600 MHz Cryoprobe

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
3	RNA (5'-R((PSU)PCPGPGPGPCPCPCPAPUPAPCPCPCPCPGP*A)-3')	natural abundance		1.2 mM
4	phosphate buffer	natural abundance		20 mM

nullKeywords ASL, Methionine, modification, RNA, tRNA