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BMRB: 16672

2KSR

NMR structures of TM domain of the n-Acetylcholine receptor b2 subunit

Authors

Bondarenko, V., Tillman, T., Xu, Y., Tang, P.

Assembly

transmembrane domain of the n-acetylcholine receptor beta2 subunit

Entity

1. transmembrane domain of the n-acetylcholine receptor beta2 subunit (polymer, Thiol state: all free), 164 monomers, 18455.86 Da Detail

MHHHHHHSTS VDLGTENLYF QSNARRKPLF YTINLIIPCV LITSLAILVF YLPSDCGEKM TLCISVLLAL TVFLLLISKI VPPTSLDVPL VGKYLMFTMV LVTFSIVTSV CVLNVHHRSP TTHTPRGGGG YVAMVIDRLF LWIFVFVCVF GTIGMFLQPL FQNY

Formula weight

18455.86 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing, torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 89.0 %, Completeness: 71.5 %, Completeness (bb): 74.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	71.5 % (1408 of 1969)	77.6 % (774 of 998)	61.2 % (494 of 807)	85.4 % (140 of 164)
Backbone	74.0 % (715 of 966)	85.7 % (281 of 328)	62.1 % (300 of 483)	86.5 % (134 of 155)
Sidechain	71.4 % (827 of 1158)	73.6 % (493 of 670)	68.5 % (328 of 479)	66.7 % (6 of 9)
Aromatic	29.2 % (63 of 216)	47.2 % (51 of 108)	10.3 % (11 of 107)	100.0 % (1 of 1)
Methyl	81.6 % (209 of 256)	82.0 % (105 of 128)	81.3 % (104 of 128)

1. transmembrane domain of the n-acetylcholine receptor beta2 subunit

MHHHHHHSTS VDLGTENLYF QSNARRKPLF YTINLIIPCV LITSLAILVF YLPSDCGEKM TLCISVLLAL TVFLLLISKI VPPTSLDVPL VGKYLMFTMV LVTFSIVTSV CVLNVHHRSP TTHTPRGGGG YVAMVIDRLF LWIFVFVCVF GTIGMFLQPL FQNY

Show sample condition

Sample #1

Solvent system 50% HFIP/50% H2O, Pressure 1 atm, Temperature 313 K, pH 3.8, Details TM123 of n-AChR b2 subunit

#	Name	Isotope labeling	Concentration
1	TM123	[U-100% 13C; U-100% 15N]	0.2 mM
2	HFIP	natural abundance	50 %
3	H2O	natural abundance	50 %

Sample #2

Solvent system 50% HFIP/ 50% H2O, Pressure 1 atm, Temperature 313 K, pH 3.8, Details TM1234 of n-AChR b2 subunit

#	Name	Isotope labeling	Concentration
4	TM1234	[U-100% 13C; U-100% 15N]	0.2 mM
5	HFIP	natural abundance	50 %
6	H2O	natural abundance	50 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.21 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.21 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.1 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2KSR, Strand ID: A Detail

Release date

2010-05-17

Citation

NMR structure of the transmembrane domain of the n-acetylcholine receptor beta2 subunit
Bondarenko, V., Tillman, T., Xu, Y., Tang, P.
Biochim. Biophys. Acta (2010), 1798, 1608-1614, PubMed 20441771 , DOI 10.1016/j.bbamem.2010.04.014 , Abstract

Related entities 1. transmembrane domain of the n-acetylcholine receptor beta2 subunit, : 1 : 11 entities Detail

Experiments performed 14 experiments Detail

1 2D 1H-15N HSQC