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BMRB: 16675

2KSV

The NMR structure of protein-glutaminase from Chryseobacterium proteolyticum

Authors

Kumeta, H., Miwa, N., Ogura, K., Kai, Y., Mizukoshi, T., Shimba, N., Suzuki, E., Inagaki, F.

Assembly

protein-glutaminase

Entity

1. protein-glutaminase (polymer, Thiol state: free and disulfide bound), 185 monomers, 19860.14 Da Detail

LASVIPDVAT LNSLFNQIKN QSCGTSTASS PCITFRYPVD GCYARAHKMR QILMNNGYDC EKQFVYGNLK ASTGTCCVAW SYHVAILVSY KNASGVTEKR IIDPSLFSSG PVTDTAWRNA CVNTSCGSAS VSSYANTAGN VYYRSPSNSY LYDNNLINTN CVLTKFSLLS GCSPSPAPDV SSCGF

Formula weight

19860.14 Da

Source organism

Chryseobacterium proteolyticum

Exptl. method

solution NMR

Refine. method

simulated annealing, torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 97.8 %, Completeness (bb): 97.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	97.8 % (1982 of 2027)	97.8 % (1012 of 1035)	97.6 % (775 of 794)	98.5 % (195 of 198)
Backbone	97.8 % (1068 of 1092)	97.6 % (363 of 372)	97.6 % (531 of 544)	98.9 % (174 of 176)
Sidechain	98.1 % (1088 of 1109)	97.9 % (649 of 663)	98.6 % (418 of 424)	95.5 % (21 of 22)
Aromatic	91.7 % (165 of 180)	90.0 % (81 of 90)	93.2 % (82 of 88)	100.0 % (2 of 2)
Methyl	99.5 % (191 of 192)	99.0 % (95 of 96)	100.0 % (96 of 96)

1. protein-glutaminase

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	protein glutaminase	[U-13C; U-15N]	1.15 mM
2	D2O	natural abundance	5 %
3	H2O	natural abundance	95 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.06 ppm, Outliers: 4 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.06 ppm, Outliers: 4 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.09 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.05 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2KSV, Strand ID: A Detail

Release date

2010-05-04

Citation

The NMR structure of protein-glutaminase from Chryseobacterium proteolyticum
Kumeta, H., Miwa, N., Ogura, K., Kai, Y., Mizukoshi, T., Shimba, N., Suzuki, E., Inagaki, F.
J. Biomol. NMR (2010), 46, 251-255, PubMed 20195702 , DOI 10.1007/s10858-010-9399-7 , Abstract

Related entities 1. protein-glutaminase, : 1 : 3 : 1 entities Detail

Experiments performed 15 experiments Detail

1 2D 1H-15N HSQC