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BMRB: 16682

2KT4

Lipocalin Q83 is a Siderocalin

Authors

Coudevylle, N., Geist, L., Hartl, M., Kontaxis, G., Bister, K., Konrat, R.

Assembly

Q83/[GaIII(Ent)]3-

Entity

1. Q83 (polymer, Thiol state: all disulfide bound), 157 monomers, 18077.50 Da Detail

MTVPDRSEIA GKWYVVALAS NTEFFLREKD KMKMAMARIS FLGEDELKVS YAVPKPNGCR KWETTFKKTS DDGEVYYSEE AKKKVEVLDT DYKSYAVIYA TRVKDGRTLH MMRLYSRSPE VSPAATAIFR KLAGERNYTD EMVAMLPRQE ECTVDEV

2. EB4 (non-polymer), 669.546 Da
3. GA (non-polymer), 69.723 Da

Total weight

18816.77 Da

Max. entity weight

18077.5 Da

Source organism

Coturnix coturnix

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 86.6 %, Completeness: 65.5 %, Completeness (bb): 84.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	65.5 % (1211 of 1850)	54.2 % (523 of 965)	75.7 % (551 of 728)	87.3 % (137 of 157)
Backbone	84.9 % (790 of 930)	84.7 % (266 of 314)	84.3 % (392 of 465)	87.4 % (132 of 151)
Sidechain	51.2 % (548 of 1071)	39.5 % (257 of 651)	69.1 % (286 of 414)	83.3 % (5 of 6)
Aromatic	16.0 % (24 of 150)	10.7 % (8 of 75)	19.2 % (14 of 73)	100.0 % (2 of 2)
Methyl	86.1 % (136 of 158)	84.8 % (67 of 79)	87.3 % (69 of 79)

1. Q83

MTVPDRSEIA GKWYVVALAS NTEFFLREKD KMKMAMARIS FLGEDELKVS YAVPKPNGCR KWETTFKKTS DDGEVYYSEE AKKKVEVLDT DYKSYAVIYA TRVKDGRTLH MMRLYSRSPE VSPAATAIFR KLAGERNYTD EMVAMLPRQE ECTVDEV

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Q83	[U-95% 13C; U-95% 15N]	1 mM
2	Enterobactin	natural abundance	1 mM
3	Gallium	natural abundance	1 mM
4	DTT	natural abundance	0.5 mM
5	potassium phosphate	natural abundance	20 mM
6	sodium chloride	natural abundance	50 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TMP	methyl protons	internal	indirect	0.2514495
¹H	TMP	methyl protons	internal	direct	1.0
¹⁵N	TMP	methyl protons	internal	indirect	0.1013291

Referencing offset: -1.0 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TMP	methyl protons	internal	indirect	0.2514495
¹H	TMP	methyl protons	internal	direct	1.0
¹⁵N	TMP	methyl protons	internal	indirect	0.1013291

Referencing offset: -1.0 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TMP	methyl protons	internal	indirect	0.2514495
¹H	TMP	methyl protons	internal	direct	1.0
¹⁵N	TMP	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.1 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TMP	methyl protons	internal	indirect	0.2514495
¹H	TMP	methyl protons	internal	direct	1.0
¹⁵N	TMP	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.47 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2KT4, Strand ID: B Detail

Release date

2010-10-17

Citation

The v-myc-induced Q83 lipocalin is a siderocalin
Coudevylle, N., Geist, L., Hotzinger, M., Hartl, M., Kontaxis, G., Bister, K., Konrat, R.
J. Biol. Chem. (2010), 285, 41646-41652, PubMed 20826777 , DOI 10.1074/jbc.M110.123331 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: Q9I9P7 released on 2002-04-16
Title EXFAB_COTJA Entity Extracellular fatty acid-binding protein

Related entities 1. Q83, : 1 : 2 : 1 : 221 entities Detail

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC