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Found 1 Document (1.371 seconds)

BMRB: 16683

2KT5

RRM domain of mRNA export adaptor REF2-I bound to HSV-1 ICP27 peptide

Authors

Tunnicliffe, R.B., Golovanov, A.P., Wilson, S.A., Hautbergue, G.M.

Assembly

REF2-I/HSV-1 ICP27 peptide

Entity

1. REF 54-155 (polymer, Thiol state: all free), 124 monomers, 13574.96 Da Detail

MASMTGGQQM GRDPDKWQHD LFDSGCGGGE GVETGAKLLV SNLDFGVSDA DIQELFAEFG TLKKAAVDYD RSGRSLGTAD VHFERRADAL KAMKQYKGVP LDGRPMDIQL VASQIDLEHH HHHH

2. ICP27 103-138 (polymer, Thiol state: all free), 40 monomers, 4259.858 Da Detail

GPLGSVWSRL GARRPSCSPE RHGGKVARLQ PPPTKAQPAR

Total weight

17834.818 Da

Max. entity weight

13574.96 Da

Source organism

Mus musculus , Human alphaherpesvirus 1

Exptl. method

solution NMR

Refine. method

TORSION ANGLE DYNAMICS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.0 %, Completeness: 93.0 %, Completeness (bb): 93.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	93.0 % (1698 of 1825)	94.3 % (901 of 955)	90.5 % (637 of 704)	96.4 % (160 of 166)
Backbone	93.0 % (897 of 964)	96.2 % (325 of 338)	89.8 % (424 of 472)	96.1 % (148 of 154)
Sidechain	93.5 % (940 of 1005)	93.4 % (576 of 617)	93.6 % (352 of 376)	100.0 % (12 of 12)
Aromatic	79.4 % (100 of 126)	79.4 % (50 of 63)	78.7 % (48 of 61)	100.0 % (2 of 2)
Methyl	98.6 % (142 of 144)	98.6 % (71 of 72)	98.6 % (71 of 72)

1. REF 54-155

MASMTGGQQM GRDPDKWQHD LFDSGCGGGE GVETGAKLLV SNLDFGVSDA DIQELFAEFG TLKKAAVDYD RSGRSLGTAD VHFERRADAL KAMKQYKGVP LDGRPMDIQL VASQIDLEHH HHHH

2. ICP27 103-138

GPLGSVWSRL GARRPSCSPE RHGGKVARLQ PPPTKAQPAR

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 (±0.1) K, pH 6.2 (±0.05), Details 13C/15N-REF 54-155 and unlabelled ICP27 103-138 (1:2)

#	Name	Isotope labeling	Concentration
1	REF 54-155	[U-99% 13C; U-99% 15N]	1 (±0.05) mM
2	ICP27 103-138	natural abundance	2 (±0.05) mM
3	Na phosphate buffer	natural abundance	20 mM
4	NaCl	natural abundance	50 mM
5	L-Arg	natural abundance	50 mM
6	L-Glu	natural abundance	50 mM
7	2-mercaptoethanol	natural abundance	50 mM
8	DTT	natural abundance	10 mM
9	EDTA	natural abundance	10 mM
10	H2O	natural abundance	90 %
11	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 (±0.1) K, pH 6.2 (±0.05), Details 13C/15N ICP27 103-138 and unlabelled REF 54-155 (1:2)

#	Name	Isotope labeling	Concentration
12	ICP27 103-138	[U-99% 13C; U-99% 15N]	1 (±0.05) mM
13	REF 54-155	natural abundance	2 (±0.05) mM
14	Na phosphate buffer	natural abundance	20 mM
15	NaCl	natural abundance	50 mM
16	L-Arg	natural abundance	50 mM
17	L-Glu	natural abundance	50 mM
18	2-mercaptoethanol	natural abundance	50 mM
19	DTT	natural abundance	10 mM
20	EDTA	natural abundance	10 mM
21	H2O	natural abundance	90 %
22	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2KT5, Strand ID: A, B Detail

Release date

2011-01-24

Citation

Structural basis for the recognition of cellular mRNA export factor REF by herpes viral proteins HSV-1 ICP27 and HVS ORF57
Tunnicliffe, R.B., Hautbergue, G.M., Kalra, P., Jackson, B.R., Whitehouse, A., Wilson, S.A., Golovanov, A.P.
PLoS Pathog. (2011), 7, e1001244-e1001244, PubMed 21253573 , DOI 10.1371/journal.ppat.1001244 , Abstract

Entries sharing articles BMRB: 7, Swiss-Prot: 4 entries Detail

  BMRB: 17693 released on 2012-06-10
    Title RRM domain of mRNA export adaptor REF2-I bound to HVS ORF57 peptide
  BMRB: 17663 released on 2011-08-31
    Title HVS ORF57 56-140 backbone assignment
  BMRB: 17664 released on 2011-08-31
    Title HVS ORF57 103-120 backbone assignment
  BMRB: 16696 released on 2011-01-24
    Title HSV-1 ICP27 (REF-interation) peptide backbone assignment in free form
  BMRB: 16697 released on 2011-01-24
    Title RRM domain of mRNA export adaptor REF2-I backbone assignment
  BMRB: 16698 released on 2011-01-24
    Title HVS ORF57 8-120 backbone assignment
  BMRB: 7435 released on 2011-01-24
    Title HVS ORF57 8-120 backbone assignment, in complex with REF2-I 54-155.
  Swiss-Prot: Q86V81 released on 2003-10-10
    Title THOC4_HUMAN Entity THO complex subunit 4
  Swiss-Prot: Q9JJW6 released on 2003-10-10
    Title ALRF2_MOUSE Entity Aly/REF export factor 2
  Swiss-Prot: P13199 released on 1990-01-01
    Title ICP27_SHV21 Entity mRNA export factor ICP27 homolog
  Swiss-Prot: P10238 released on 1989-07-01
    Title ICP27_HHV11 Entity mRNA export factor

Related entities 1. REF 54-155, : 31 entities Detail

Related entities 2. ICP27 103-138, : 1 : 4 entities Detail

Interaction partners 2. ICP27 103-138, : 6 interactors Detail

More details for 6 interactors identified by 4 citations

Experiments performed 8 experiments Detail

1 3D 13C-separated NOESY

Instrument

Varian INOVA - 800 MHz Used for filtered NOESY spectra

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 (±0.1) K, pH 6.2 (±0.05), Details 13C/15N-REF 54-155 and unlabelled ICP27 103-138 (1:2)

#	Name	Isotope labeling	Concentration
1	REF 54-155	[U-99% 13C; U-99% 15N]	1 (±0.05) mM
2	ICP27 103-138	natural abundance	2 (±0.05) mM
3	Na phosphate buffer	natural abundance	20 mM
4	NaCl	natural abundance	50 mM
5	L-Arg	natural abundance	50 mM
6	L-Glu	natural abundance	50 mM
7	2-mercaptoethanol	natural abundance	50 mM
8	DTT	natural abundance	10 mM
9	EDTA	natural abundance	10 mM
10	H2O	natural abundance	90 %
11	D2O	natural abundance	10 %

2 3D 15N-separated NOESY

Instrument

Bruker DRX - 600 MHz Majority of spectra acquired on 600

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 (±0.1) K, pH 6.2 (±0.05), Details 13C/15N-REF 54-155 and unlabelled ICP27 103-138 (1:2)

#	Name	Isotope labeling	Concentration
1	REF 54-155	[U-99% 13C; U-99% 15N]	1 (±0.05) mM
2	ICP27 103-138	natural abundance	2 (±0.05) mM
3	Na phosphate buffer	natural abundance	20 mM
4	NaCl	natural abundance	50 mM
5	L-Arg	natural abundance	50 mM
6	L-Glu	natural abundance	50 mM
7	2-mercaptoethanol	natural abundance	50 mM
8	DTT	natural abundance	10 mM
9	EDTA	natural abundance	10 mM
10	H2O	natural abundance	90 %
11	D2O	natural abundance	10 %

3 filtered 13C-NOESY

Instrument

Varian INOVA - 800 MHz Used for filtered NOESY spectra

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 (±0.1) K, pH 6.2 (±0.05), Details 13C/15N-REF 54-155 and unlabelled ICP27 103-138 (1:2)

#	Name	Isotope labeling	Concentration
1	REF 54-155	[U-99% 13C; U-99% 15N]	1 (±0.05) mM
2	ICP27 103-138	natural abundance	2 (±0.05) mM
3	Na phosphate buffer	natural abundance	20 mM
4	NaCl	natural abundance	50 mM
5	L-Arg	natural abundance	50 mM
6	L-Glu	natural abundance	50 mM
7	2-mercaptoethanol	natural abundance	50 mM
8	DTT	natural abundance	10 mM
9	EDTA	natural abundance	10 mM
10	H2O	natural abundance	90 %
11	D2O	natural abundance	10 %

4 filtered 13C-NOESY

Instrument

Varian INOVA - 800 MHz Used for filtered NOESY spectra

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 (±0.1) K, pH 6.2 (±0.05), Details 13C/15N ICP27 103-138 and unlabelled REF 54-155 (1:2)

#	Name	Isotope labeling	Concentration
12	ICP27 103-138	[U-99% 13C; U-99% 15N]	1 (±0.05) mM
13	REF 54-155	natural abundance	2 (±0.05) mM
14	Na phosphate buffer	natural abundance	20 mM
15	NaCl	natural abundance	50 mM
16	L-Arg	natural abundance	50 mM
17	L-Glu	natural abundance	50 mM
18	2-mercaptoethanol	natural abundance	50 mM
19	DTT	natural abundance	10 mM
20	EDTA	natural abundance	10 mM
21	H2O	natural abundance	90 %
22	D2O	natural abundance	10 %

5 3D 13C-separated NOESY

Instrument

Bruker DRX - 600 MHz Majority of spectra acquired on 600

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 (±0.1) K, pH 6.2 (±0.05), Details 13C/15N ICP27 103-138 and unlabelled REF 54-155 (1:2)

#	Name	Isotope labeling	Concentration
12	ICP27 103-138	[U-99% 13C; U-99% 15N]	1 (±0.05) mM
13	REF 54-155	natural abundance	2 (±0.05) mM
14	Na phosphate buffer	natural abundance	20 mM
15	NaCl	natural abundance	50 mM
16	L-Arg	natural abundance	50 mM
17	L-Glu	natural abundance	50 mM
18	2-mercaptoethanol	natural abundance	50 mM
19	DTT	natural abundance	10 mM
20	EDTA	natural abundance	10 mM
21	H2O	natural abundance	90 %
22	D2O	natural abundance	10 %

6 3D 15N-separated NOESY

Instrument

Bruker Avance II+ - 700 MHz Used for carbonyl backbone assignment spectra

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 (±0.1) K, pH 6.2 (±0.05), Details 13C/15N ICP27 103-138 and unlabelled REF 54-155 (1:2)

#	Name	Isotope labeling	Concentration
12	ICP27 103-138	[U-99% 13C; U-99% 15N]	1 (±0.05) mM
13	REF 54-155	natural abundance	2 (±0.05) mM
14	Na phosphate buffer	natural abundance	20 mM
15	NaCl	natural abundance	50 mM
16	L-Arg	natural abundance	50 mM
17	L-Glu	natural abundance	50 mM
18	2-mercaptoethanol	natural abundance	50 mM
19	DTT	natural abundance	10 mM
20	EDTA	natural abundance	10 mM
21	H2O	natural abundance	90 %
22	D2O	natural abundance	10 %

7 3D Standard triple-resonance

Instrument

Bruker DRX - 600 MHz Majority of spectra acquired on 600

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 (±0.1) K, pH 6.2 (±0.05), Details 13C/15N-REF 54-155 and unlabelled ICP27 103-138 (1:2)

#	Name	Isotope labeling	Concentration
1	REF 54-155	[U-99% 13C; U-99% 15N]	1 (±0.05) mM
2	ICP27 103-138	natural abundance	2 (±0.05) mM
3	Na phosphate buffer	natural abundance	20 mM
4	NaCl	natural abundance	50 mM
5	L-Arg	natural abundance	50 mM
6	L-Glu	natural abundance	50 mM
7	2-mercaptoethanol	natural abundance	50 mM
8	DTT	natural abundance	10 mM
9	EDTA	natural abundance	10 mM
10	H2O	natural abundance	90 %
11	D2O	natural abundance	10 %

8 3d Standard triple-resonance

Instrument

Bruker DRX - 600 MHz Majority of spectra acquired on 600

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 (±0.1) K, pH 6.2 (±0.05), Details 13C/15N ICP27 103-138 and unlabelled REF 54-155 (1:2)

#	Name	Isotope labeling	Concentration
12	ICP27 103-138	[U-99% 13C; U-99% 15N]	1 (±0.05) mM
13	REF 54-155	natural abundance	2 (±0.05) mM
14	Na phosphate buffer	natural abundance	20 mM
15	NaCl	natural abundance	50 mM
16	L-Arg	natural abundance	50 mM
17	L-Glu	natural abundance	50 mM
18	2-mercaptoethanol	natural abundance	50 mM
19	DTT	natural abundance	10 mM
20	EDTA	natural abundance	10 mM
21	H2O	natural abundance	90 %
22	D2O	natural abundance	10 %

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Found 1 Document (1.371 seconds)

BMRB: 16683

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles BMRB: 7, Swiss-Prot: 4 entries Detail

Related entities 1. REF 54-155, : 31 entities Detail

Related entities 2. ICP27 103-138, : 1 : 4 entities Detail

Interaction partners 2. ICP27 103-138, : 6 interactors Detail

Experiments performed 8 experiments Detail

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