BMRBj - BMREntryMaster

Found 1 Document (0.514 seconds)

BMRB: 16686

2KT7

Solution NMR structure of mucin-binding domain of protein lmo0835 from Listeria monocytogenes. Northeast Structural Genomics Consortium Target LmR64A

Authors

Eletsky, A., He, Y., Lee, D., Ciccosanti, C., Janjua, H., Acton, T.B., Xiao, R., Everett, J.K., Montelione, G.T., Szyperski, T.

Assembly

lmo0835

Entity

1. lmo0835 (polymer, Thiol state: not present), 102 monomers, 11486.59 Da Detail

MDTNNFTVKV EYVDADGAEI APSDTLTDYH YVSTPKDIPG YKLREIPHNA TGNITDTGII VRYIYDKIID VSYVDETGKD LLPVVEIINS EAAVLEHHHH HH

Formula weight

11486.59 Da

Source organism

Listeria monocytogenes

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.1 %, Completeness: 93.0 %, Completeness (bb): 94.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	93.0 % (1072 of 1153)	92.8 % (544 of 586)	92.9 % (432 of 465)	94.1 % (96 of 102)
Backbone	94.2 % (567 of 602)	93.1 % (190 of 204)	95.0 % (286 of 301)	93.8 % (91 of 97)
Sidechain	92.1 % (597 of 648)	92.7 % (354 of 382)	91.2 % (238 of 261)	100.0 % (5 of 5)
Aromatic	70.4 % (69 of 98)	75.5 % (37 of 49)	65.3 % (32 of 49)
Methyl	100.0 % (134 of 134)	100.0 % (67 of 67)	100.0 % (67 of 67)

1. lmr64a protein

MDTNNFTVKV EYVDADGAEI APSDTLTDYH YVSTPKDIPG YKLREIPHNA TGNITDTGII VRYIYDKIID VSYVDETGKD LLPVVEIINS EAAVLEHHHH HH

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	lmr64a_protein	[U-13C; U-15N]	0.9 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	calcium chloride	natural abundance	5 mM
5	sodium azide	natural abundance	0.01 %
6	H2O	natural abundance	95 %
7	D2O	natural abundance	5 %

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
8	lmr64a_protein	[U-5% 13C; U-15N]	0.9 mM
9	MES	natural abundance	20 mM
10	sodium chloride	natural abundance	100 mM
11	calcium chloride	natural abundance	5 mM
12	sodium azide	natural abundance	0.01 %
13	H2O	natural abundance	95 %
14	D2O	natural abundance	5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.06 ppm, Outliers: 5 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.06 ppm, Outliers: 5 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.49 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2KT7, Strand ID: A Detail

Release date

2010-02-24

Citation

Solution NMR structure of mucin-binding domain of protein lmo0835 from Listeria monocytogenes
Eletsky, A., He, Y., Lee, D., Ciccosanti, C., Janjua, H., Acton, T.B., Xiao, R., Everett, J.K., Montelione, G.T., Szyperski, T.
Proteins: Struct. Funct. Genet.

Related entities 1. lmo0835, : 1 : 1 entities Detail

Experiments performed 16 experiments Detail

1 1D 15N T1