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BMRB: 16701

2LGZ

Backbone 1H, 13C, and 15N Chemical Shift Assignments for the C-terminal domain of Stt3p

Authors

Huang, C., Mohanty, S.

Assembly

the C-terminal domain of Stt3p

Entity

1. the C-terminal domain of Stt3p (polymer, Thiol state: not present), 273 monomers, 31370.61 Da Detail

MGSSHHHHHH SSGLVPRGSH STWVTRTAYS SPSVVLPSQT PDGKLALIDD FREAYYWLRM NSDEDSKVAA WWDYGYQIGG MADRTTLVDN NTWNNTHIAI VGKANASPEE KSYEILKEHD VDYVLVIFGG LIGFGGDDIN KFLWNIRISE GIWPEEIKER DFYTAEGEYR VDARASETMR NSLLYKMSYK DFPQLFNGGQ ATDRVRQQMI TPLDVPPLDY FDEVFTSENW NVRIYQLKKD DAQGRTLRDV GELTRSSTKT RRSIKRPELG LRV

Formula weight

31370.61 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.1 %, Completeness: 43.8 %, Completeness (bb): 77.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	43.8 % (1401 of 3200)	25.2 % (419 of 1662)	59.5 % (742 of 1248)	82.8 % (240 of 290)
Backbone	77.0 % (1244 of 1616)	52.3 % (290 of 555)	89.6 % (716 of 799)	90.8 % (238 of 262)
Sidechain	21.0 % (385 of 1837)	11.7 % (129 of 1107)	36.2 % (254 of 702)	7.1 % (2 of 28)
Aromatic	4.9 % (16 of 326)	6.1 % (10 of 163)	3.2 % (5 of 155)	12.5 % (1 of 8)
Methyl	18.9 % (51 of 270)	17.8 % (24 of 135)	20.0 % (27 of 135)

1. the C-terminal domain of Stt3p

MGSSHHHHHH SSGLVPRGSH STWVTRTAYS SPSVVLPSQT PDGKLALIDD FREAYYWLRM NSDEDSKVAA WWDYGYQIGG MADRTTLVDN NTWNNTHIAI VGKANASPEE KSYEILKEHD VDYVLVIFGG LIGFGGDDIN KFLWNIRISE GIWPEEIKER DFYTAEGEYR VDARASETMR NSLLYKMSYK DFPQLFNGGQ ATDRVRQQMI TPLDVPPLDY FDEVFTSENW NVRIYQLKKD DAQGRTLRDV GELTRSSTKT RRSIKRPELG LRV

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 328 K, pH 6.5, Details 2H, 15N, 13C-triple labeled sample in 100mM D-SDS, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	the C-terminal domain of Stt3p	[U-13C; U-15N; U-2H]		600 mM
2	SDS	[U-2H]		100 mM

Protein Blocks Logo

Calculated from 10 models in PDB: 2LGZ, Strand ID: A Detail

Release date

2010-03-25

Citation

Challenging the limit: NMR assignment of a 31 kDa helical membrane protein
Huang, C., Mohanty, S.
J. Am. Chem. Soc. (2010), 132, 3662-3663, PubMed 20178386 , DOI 10.1021/ja100078z , Abstract

Related entities 1. the C-terminal domain of Stt3p, : 1 : 5 : 43 entities Detail

Interaction partners 1. the C-terminal domain of Stt3p, : 18 interactors Detail

More details for 18 interactors identified by 9 citations

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 600 MHz At Auburn University

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 328 K, pH 6.5, Details 2H, 15N, 13C-triple labeled sample in 100mM D-SDS, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	the C-terminal domain of Stt3p	[U-13C; U-15N; U-2H]		600 mM
2	SDS	[U-2H]		100 mM

2 3D HNCO

Instrument

Bruker Avance - 600 MHz At Auburn University

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 328 K, pH 6.5, Details 2H, 15N, 13C-triple labeled sample in 100mM D-SDS, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	the C-terminal domain of Stt3p	[U-13C; U-15N; U-2H]		600 mM
2	SDS	[U-2H]		100 mM

3 3D HN(COCA)CB

Instrument

Bruker Avance - 600 MHz At Auburn University

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 328 K, pH 6.5, Details 2H, 15N, 13C-triple labeled sample in 100mM D-SDS, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	the C-terminal domain of Stt3p	[U-13C; U-15N; U-2H]		600 mM
2	SDS	[U-2H]		100 mM

4 3D HN(CO)CA

Instrument

Bruker Avance - 600 MHz At Auburn University

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 328 K, pH 6.5, Details 2H, 15N, 13C-triple labeled sample in 100mM D-SDS, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	the C-terminal domain of Stt3p	[U-13C; U-15N; U-2H]		600 mM
2	SDS	[U-2H]		100 mM

5 3D HNCA

Instrument

Bruker Avance - 600 MHz At Auburn University

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 328 K, pH 6.5, Details 2H, 15N, 13C-triple labeled sample in 100mM D-SDS, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	the C-terminal domain of Stt3p	[U-13C; U-15N; U-2H]		600 mM
2	SDS	[U-2H]		100 mM

6 3D HN(CA)CO

Instrument

Bruker Avance - 600 MHz At Auburn University

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 328 K, pH 6.5, Details 2H, 15N, 13C-triple labeled sample in 100mM D-SDS, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	the C-terminal domain of Stt3p	[U-13C; U-15N; U-2H]		600 mM
2	SDS	[U-2H]		100 mM

7 3D HNCACB

Instrument

Bruker Avance - 600 MHz At Auburn University

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 328 K, pH 6.5, Details 2H, 15N, 13C-triple labeled sample in 100mM D-SDS, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	the C-terminal domain of Stt3p	[U-13C; U-15N; U-2H]		600 mM
2	SDS	[U-2H]		100 mM

8 2D 1H-15N HSQC

Instrument

Varian INOVA - 900 MHz At the University of Georgia

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 328 K, pH 6.5, Details 2H, 15N, 13C-triple labeled sample in 100mM D-SDS, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	the C-terminal domain of Stt3p	[U-13C; U-15N; U-2H]		600 mM
2	SDS	[U-2H]		100 mM

9 3D HNCACB

Instrument

Varian INOVA - 900 MHz At the University of Georgia

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 328 K, pH 6.5, Details 2H, 15N, 13C-triple labeled sample in 100mM D-SDS, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	the C-terminal domain of Stt3p	[U-13C; U-15N; U-2H]		600 mM
2	SDS	[U-2H]		100 mM

10 3D HN(CA)CO

Instrument

Varian INOVA - 900 MHz At the University of Georgia

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 328 K, pH 6.5, Details 2H, 15N, 13C-triple labeled sample in 100mM D-SDS, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	the C-terminal domain of Stt3p	[U-13C; U-15N; U-2H]		600 mM
2	SDS	[U-2H]		100 mM

nullKeywords membrane protein, N-glycosylation, NMR assignment, Oligosaccharyl Transferase, Stt3p