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BMRB: 16711

2KTS

NMR structure of the protein NP_415897.1

Authors

Serrano, P., Jaudzems, K., Geralt, M., Horst, R., Wuthrich, K., Wilson, I.A.

Assembly

NP 415897.1

Entity

1. NP 415897.1 (polymer, Thiol state: all free), 117 monomers, 12833.64 Da Detail

MVTPEQLQHH RFVLESVNGK PVTSDKNPPE ISFGEKMMIS GSMCNRFSGE GKLSNGELTA KGLAMTRMMC ANPQLNELDN TISEMLKEGA QVDLTANQLT LATAKQTLTY KLADLMN

Formula weight

12833.64 Da

Source organism

Escherichia coli

Exptl. method

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 90.2 %, Completeness (bb): 84.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	90.2 % (1202 of 1333)	96.6 % (672 of 696)	79.2 % (404 of 510)	99.2 % (126 of 127)
Backbone	84.4 % (584 of 692)	97.9 % (232 of 237)	70.6 % (242 of 343)	98.2 % (110 of 112)
Sidechain	96.7 % (725 of 750)	95.9 % (440 of 459)	97.8 % (270 of 276)	100.0 % (15 of 15)
Aromatic	91.3 % (42 of 46)	100.0 % (23 of 23)	82.6 % (19 of 23)
Methyl	100.0 % (124 of 124)	100.0 % (62 of 62)	100.0 % (62 of 62)

1. NP 415897.1

MVTPEQLQHH RFVLESVNGK PVTSDKNPPE ISFGEKMMIS GSMCNRFSGE GKLSNGELTA KGLAMTRMMC ANPQLNELDN TISEMLKEGA QVDLTANQLT LATAKQTLTY KLADLMN

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	NP_415897.1	[U-98% 13C; U-98% 15N]		1.2 mM
2	sodium phosphate	natural abundance		20 mM
3	sodium chloride	natural abundance		50 mM
4	sodium azide	natural abundance		0.05 %
5	H2O	natural abundance		95 %
6	D2O	natural abundance		5 %

LACS Plot; CA

Referencing offset: -0.48 ppm, Outliers: 1 Detail

LACS Plot; CB

Referencing offset: -0.48 ppm, Outliers: 1 Detail

LACS Plot; HA

Referencing offset: -0.07 ppm, Outliers: 4 Detail

Protein Blocks Logo

Calculated from 19 models in PDB: 2KTS, Strand ID: A Detail

Release date

2012-08-02

Citation

NMR structure of the protein NP_415897.1
Serrano, P., Jaudzems, K., Geralt, M., Horst, R., Wilson, I.A., Wuthrich, K.
Not known

Related entities 1. NP 415897.1, : 1 : 2 entities Detail

Experiments performed 8 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	NP_415897.1	[U-98% 13C; U-98% 15N]		1.2 mM
2	sodium phosphate	natural abundance		20 mM
3	sodium chloride	natural abundance		50 mM
4	sodium azide	natural abundance		0.05 %
5	H2O	natural abundance		95 %
6	D2O	natural abundance		5 %

2 2D 1H-13C HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	NP_415897.1	[U-98% 13C; U-98% 15N]		1.2 mM
2	sodium phosphate	natural abundance		20 mM
3	sodium chloride	natural abundance		50 mM
4	sodium azide	natural abundance		0.05 %
5	H2O	natural abundance		95 %
6	D2O	natural abundance		5 %

3 3D 1H-15N NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	NP_415897.1	[U-98% 13C; U-98% 15N]		1.2 mM
2	sodium phosphate	natural abundance		20 mM
3	sodium chloride	natural abundance		50 mM
4	sodium azide	natural abundance		0.05 %
5	H2O	natural abundance		95 %
6	D2O	natural abundance		5 %

4 3D 1H-13C NOESY (Ali)

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	NP_415897.1	[U-98% 13C; U-98% 15N]		1.2 mM
2	sodium phosphate	natural abundance		20 mM
3	sodium chloride	natural abundance		50 mM
4	sodium azide	natural abundance		0.05 %
5	H2O	natural abundance		95 %
6	D2O	natural abundance		5 %

5 3D 1H-13C NOESY (Aro)

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	NP_415897.1	[U-98% 13C; U-98% 15N]		1.2 mM
2	sodium phosphate	natural abundance		20 mM
3	sodium chloride	natural abundance		50 mM
4	sodium azide	natural abundance		0.05 %
5	H2O	natural abundance		95 %
6	D2O	natural abundance		5 %

6 APSY 5D-CBCACONH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	NP_415897.1	[U-98% 13C; U-98% 15N]		1.2 mM
2	sodium phosphate	natural abundance		20 mM
3	sodium chloride	natural abundance		50 mM
4	sodium azide	natural abundance		0.05 %
5	H2O	natural abundance		95 %
6	D2O	natural abundance		5 %

7 APSY 5D-HACACONH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	NP_415897.1	[U-98% 13C; U-98% 15N]		1.2 mM
2	sodium phosphate	natural abundance		20 mM
3	sodium chloride	natural abundance		50 mM
4	sodium azide	natural abundance		0.05 %
5	H2O	natural abundance		95 %
6	D2O	natural abundance		5 %

8 APSY 4D-HACANH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	NP_415897.1	[U-98% 13C; U-98% 15N]		1.2 mM
2	sodium phosphate	natural abundance		20 mM
3	sodium chloride	natural abundance		50 mM
4	sodium azide	natural abundance		0.05 %
5	H2O	natural abundance		95 %
6	D2O	natural abundance		5 %

nullKeywords JCSG, NMR, NP_415897.1