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BMRB: 16719

2L95

Solution structure of Cytotoxic T-Lymphocyte Antigent-2(CTLA-2) like protein, Crammer

Authors

Tzeng, T., Cheng, C., Liu, Y., Shih, M., Lyu, P.

Assembly

Crammer

Entity

1. Crammer (polymer, Thiol state: all free), 80 monomers, 9578.678 Da Detail

MMSLVSDEEW VEYKSKFDKN YEAEEDLMRR RIYAESKARI EEHNRKFEKG EVTWKMGINH LADLTPEEFA QRSGKKVPPN

Formula weight

9578.678 Da

Source organism

Drosophila melanogaster

Exptl. method

solution NMR

Refine. method

SIMULATED ANNEALING, MOLECULAR DYNAMICS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.0 %, Completeness: 62.6 %, Completeness (bb): 92.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	62.6 % (615 of 983)	61.4 % (321 of 523)	58.8 % (221 of 376)	86.9 % (73 of 84)
Backbone	92.4 % (438 of 474)	93.1 % (149 of 160)	91.1 % (216 of 237)	94.8 % (73 of 77)
Sidechain	41.8 % (245 of 586)	47.4 % (172 of 363)	33.8 % (73 of 216)	0.0 % (0 of 7)
Aromatic	12.8 % (11 of 86)	25.6 % (11 of 43)	0.0 % (0 of 41)	0.0 % (0 of 2)
Methyl	48.3 % (28 of 58)	72.4 % (21 of 29)	24.1 % (7 of 29)

1. Crammer

MMSLVSDEEW VEYKSKFDKN YEAEEDLMRR RIYAESKARI EEHNRKFEKG EVTWKMGINH LADLTPEEFA QRSGKKVPPN

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	entity	[U-100% 13C; U-100% 15N]		10 mM
2	Citric acid-phosphate	natural abundance		10 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6

#	Name	Isotope labeling	Type	Concentration
3	entity	[U-100% 15N]		10 mM
4	Citric acid-phosphate	natural abundance		10 mM

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6

#	Name	Isotope labeling	Type	Concentration
5	entity	natural abundance		10 mM
6	Citric acid-phosphate	natural abundance		10 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹⁵C	TSP-d4	methyl protons	0.011 ppm	internal	null	0.1013291
¹H	TSP-d4	methyl protons	0.1 ppm	internal	null	1.0
¹³N	TSP-d4	methyl protons	0.024 ppm	internal	null	0.2514502

Referencing offset: 2.34 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹⁵C	TSP-d4	methyl protons	0.011 ppm	internal	null	0.1013291
¹H	TSP-d4	methyl protons	0.1 ppm	internal	null	1.0
¹³N	TSP-d4	methyl protons	0.024 ppm	internal	null	0.2514502

Referencing offset: 2.34 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹⁵C	TSP-d4	methyl protons	0.011 ppm	internal	null	0.1013291
¹H	TSP-d4	methyl protons	0.1 ppm	internal	null	1.0
¹³N	TSP-d4	methyl protons	0.024 ppm	internal	null	0.2514502

Referencing offset: 0.18 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹⁵C	TSP-d4	methyl protons	0.011 ppm	internal	null	0.1013291
¹H	TSP-d4	methyl protons	0.1 ppm	internal	null	1.0
¹³N	TSP-d4	methyl protons	0.024 ppm	internal	null	0.2514502

Referencing offset: 2.25 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2L95, Strand ID: A Detail

Release date

2011-12-19

Citation

A molten globule-to-ordered structure transition of Drosophila melanogaster crammer is required for its ability to inhibit cathepsin
Tseng, T., Cheng, C., Chen, D., Shih, M., Liu, Y., Hsu, S., Lyu, P.
Biochem. J. (2012), 442, 563-572, PubMed 22150223 , DOI 10.1042/BJ20111360 , Abstract

Related entities 1. Crammer, : 1 : 1 : 133 entities Detail

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC