BMRB: 16720

Horse prion protein

Authors

Perez, D., Damberger, F., Wuthrich, K.

Assembly

mouse prion protein mutant D167S

Entity

1. mouse prion protein mutant D167S (polymer, Thiol state: all disulfide bound), 113 monomers, 13188.57 Da Detail

GSVVGGLGGY MLGSAMSRPL IHFGNDYEDR YYRENMYRYP NQVYYRPVSE YSNQKNFVHD CVNITVKQHT VTTTTKGENF TETDVKIMER VVEQMCITQY QKEYEAFQQR GAS

Formula weight

13188.57 Da

Entity Connection

disulfide 1 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS61:SG	1:CYS96:SG

Source organism

Equus caballus

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 70.1 %, Completeness (bb): 67.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	70.1 % (935 of 1334)	63.6 % (442 of 695)	71.8 % (369 of 514)	99.2 % (124 of 125)
Backbone	67.7 % (455 of 672)	54.3 % (126 of 232)	66.7 % (220 of 330)	99.1 % (109 of 110)
Sidechain	76.2 % (584 of 766)	68.3 % (316 of 463)	87.8 % (253 of 288)	100.0 % (15 of 15)
Aromatic	32.9 % (46 of 140)	15.7 % (11 of 70)	50.0 % (35 of 70)
Methyl	52.1 % (50 of 96)	4.2 % (2 of 48)	100.0 % (48 of 48)

1. mouse prion protein mutant D167S

GSVVGGLGGY MLGSAMSRPL IHFGNDYEDR YYRENMYRYP NQVYYRPVSE YSNQKNFVHD CVNITVKQHT VTTTTKGENF TETDVKIMER VVEQMCITQY QKEYEAFQQR GAS

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	sodium acetate	[U-2H]		10 (±1.0) mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %
4	entity	natural abundance		1.3 (±1.0) mM

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LACS Plot; CA

Referencing offset: 0.21 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	internal	indirect	0.25145
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	internal	indirect	0.10133

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LACS Plot; CB

Referencing offset: 0.21 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	internal	indirect	0.25145
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	internal	indirect	0.10133

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LACS Plot; HA

Referencing offset: 0.19 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	internal	indirect	0.25145
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	internal	indirect	0.10133

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Protein Blocks Logo

Calculated from 20 models in PDB: 2KU4, Strand ID: A Detail

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Release date

2010-05-17

Citation

Horse prion protein NMR structure and comparisons with related variants of the mouse prion protein
Perez, D.R., Damberger, F.F., Wuthrich, K.
J. Mol. Biol. (2010), 400, 121-128, PubMed 20460128 , DOI 10.1016/j.jmb.2010.04.066 , Abstract

Entries sharing articles BMRB: 2 entries Detail

Related entities 1. mouse prion protein mutant D167S, : 1 : 161 entities Detail

Interaction partners 1. mouse prion protein mutant D167S, : 1 interactors Detail

Experiments performed 5 experiments Detail