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BMRB: 16767

2KV3

Human Regenerating Gene Type IV (REG IV) PROTEIN, P91S mutant

Authors

Ho, M., Lou, Y., Chen, C.

Assembly

Reg IV

Entity

1. Reg IV (polymer, Thiol state: all disulfide bound), 130 monomers, 15208.85 Da Detail

QSCAPGWFYH KSNCYGYFRK LRNWSDAELE CQSYGNGAHL ASILSLKEAS TIAEYISGYQ RSQSIWIGLH DPQKRQQWQW IDGAMYLYRS WSGKSMGGNK HCAEMSSNNN FLTWSSNECN KRQHFLCKYR

Formula weight

15208.85 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

DGSA-distance geometry simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.2 %, Completeness: 90.1 %, Completeness (bb): 97.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	90.1 % (1408 of 1563)	89.6 % (734 of 819)	89.5 % (529 of 591)	94.8 % (145 of 153)
Backbone	97.2 % (754 of 776)	97.0 % (260 of 268)	97.4 % (370 of 380)	96.9 % (124 of 128)
Sidechain	85.1 % (772 of 907)	86.0 % (474 of 551)	83.7 % (277 of 331)	84.0 % (21 of 25)
Aromatic	60.2 % (130 of 216)	61.1 % (66 of 108)	57.4 % (58 of 101)	85.7 % (6 of 7)
Methyl	95.0 % (76 of 80)	95.0 % (38 of 40)	95.0 % (38 of 40)

1. P91S

QSCAPGWFYH KSNCYGYFRK LRNWSDAELE CQSYGNGAHL ASILSLKEAS TIAEYISGYQ RSQSIWIGLH DPQKRQQWQW IDGAMYLYRS WSGKSMGGNK HCAEMSSNNN FLTWSSNECN KRQHFLCKYR

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 4.5

#	Name	Isotope labeling	Concentration
1	Human Regenerating Gene Type IV	[U-99% 15N]	1 mM
2	Human Regenerating Gene Type IV	[U-99% 13C; U-99% 15N]	1 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.71 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.71 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.54 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2KV3, Strand ID: A Detail

Release date

2010-08-11

Citation

Human RegIV protein adopts a typical C-type lectin fold but binds mannan with two calcium-independent sites
Ho, M., Lou, Y., Wei, S., Luo, S., Lin, W., Lyu, P., Chen, C.
J. Mol. Biol. (2010), 402, 682-695, PubMed 20692269 , DOI 10.1016/j.jmb.2010.07.061 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: Q9BYZ8 released on 2005-07-05
Title REG4_HUMAN Entity Regenerating islet-derived protein 4

Related entities 1. Reg IV, : 1 : 2 : 348 entities Detail

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC