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BMRB: 16768

2KV4

EGF

Authors

Hsiao Wen, H., Mohan, S.K., Chin, Y.

Assembly

hEGF

Entity

1. hEGF (polymer, Thiol state: all disulfide bound), 53 monomers, 6221.968 Da Detail

NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR

Formula weight

6221.968 Da

Entity Connection

disulfide 3 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS6:SG	1:CYS20:SG
2	disulfide	sing	1:CYS14:SG	1:CYS31:SG
3	disulfide	sing	1:CYS33:SG	1:CYS42:SG

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.2 %, Completeness: 73.9 %, Completeness (bb): 92.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	73.9 % (450 of 609)	69.5 % (219 of 315)	77.2 % (183 of 237)	84.2 % (48 of 57)
Backbone	92.1 % (291 of 316)	89.9 % (98 of 109)	93.5 % (145 of 155)	92.3 % (48 of 52)
Sidechain	60.2 % (206 of 342)	58.7 % (121 of 206)	64.9 % (85 of 131)	0.0 % (0 of 5)
Aromatic	0.0 % (0 of 72)	0.0 % (0 of 36)	0.0 % (0 of 34)	0.0 % (0 of 2)
Methyl	84.1 % (37 of 44)	86.4 % (19 of 22)	81.8 % (18 of 22)

1. hEGF

NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	entity	[U-100% 13C; U-100% 15N]	1 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	20 mM
4	H2O	natural abundance	90 %
5	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	dioxane	methylene carbon	67.4 ppm	internal	direct	1.0
¹H	TSP	methyl hydrogen	0.0 ppm	external	direct	1.0
¹⁵N	liquid anhydrous ammonia	nitrogen	0.0 ppm	internal	direct	1.0

Referencing offset: -0.16 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	dioxane	methylene carbon	67.4 ppm	internal	direct	1.0
¹H	TSP	methyl hydrogen	0.0 ppm	external	direct	1.0
¹⁵N	liquid anhydrous ammonia	nitrogen	0.0 ppm	internal	direct	1.0

Referencing offset: -0.16 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	dioxane	methylene carbon	67.4 ppm	internal	direct	1.0
¹H	TSP	methyl hydrogen	0.0 ppm	external	direct	1.0
¹⁵N	liquid anhydrous ammonia	nitrogen	0.0 ppm	internal	direct	1.0

Referencing offset: 0.21 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	dioxane	methylene carbon	67.4 ppm	internal	direct	1.0
¹H	TSP	methyl hydrogen	0.0 ppm	external	direct	1.0
¹⁵N	liquid anhydrous ammonia	nitrogen	0.0 ppm	internal	direct	1.0

Referencing offset: 0.56 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2KV4, Strand ID: A Detail

Release date

2011-11-27

Citation

The NMR solution structure of human epidermal growth factor (hEGF) at physiological pH and its interactions with suramin
Huang, H., Mohan, S.K., Yu, C.
Biochem. Biophys. Res. Commun. (2010), 402, 705-710, PubMed 21029725 , DOI 10.1016/j.bbrc.2010.10.089 , Abstract

Related entities 1. hEGF, : 1 : 15 : 2 : 96 entities Detail

Interaction partners 1. hEGF, : 7 interactors Detail

More details for 7 interactors identified by 4 citations