BMRBj - BMREntryMaster

Found 1 Document (0.734 seconds)

BMRB: 16775

2KVD

Mesencephalic astrocyte-derived neurotrophic factor (MANF)

Authors

Hellman, M.H., Peranen, J., Saarma, M., Permi, P.

Assembly

MANF

Entity

1. MANF (polymer, Thiol state: all disulfide bound), 160 monomers, 18305.99 Da Detail

GSLRPGDCEV CISYLGRFYQ DLKDRDVTFS PATIENELIK FCREARGKEN RLCYYIGATD DAATKIINEV SKPLAHHIPV EKICEKLKKK DSQICELKYD KQIDLSTVDL KKLRVKELKK ILDDWGETCK GCAEKSDYIR KINELMPKYA PKAASARTDL

Formula weight

18305.99 Da

Source organism

Exptl. method

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 89.1 %, Completeness (bb): 82.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	89.1 % (1712 of 1922)	95.1 % (960 of 1009)	79.8 % (592 of 742)	93.6 % (160 of 171)
Backbone	82.1 % (778 of 948)	94.7 % (304 of 321)	68.7 % (325 of 473)	96.8 % (149 of 154)
Sidechain	95.5 % (1076 of 1127)	94.8 % (652 of 688)	98.1 % (414 of 422)	58.8 % (10 of 17)
Aromatic	99.1 % (105 of 106)	100.0 % (53 of 53)	98.1 % (51 of 52)	100.0 % (1 of 1)
Methyl	100.0 % (172 of 172)	100.0 % (86 of 86)	100.0 % (86 of 86)

1. MANF

GSLRPGDCEV CISYLGRFYQ DLKDRDVTFS PATIENELIK FCREARGKEN RLCYYIGATD DAATKIINEV SKPLAHHIPV EKICEKLKKK DSQICELKYD KQIDLSTVDL KKLRVKELKK ILDDWGETCK GCAEKSDYIR KINELMPKYA PKAASARTDL

Show sample condition

Sample

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 308 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	MANF	[U-98% 13C; U-98% 15N]		1 mM
2	H2O	natural abundance		93 %
3	D2O	natural abundance		7 %

Release date

2010-03-11

Citation

Mesencephalic astrocyte-derived neurotrophic factor (MANF) has a unique mechanism to rescue apoptotic neurons
Hellman, M.H., Arumae, U., Yu, L., Lindholm, P., Peranen, J., Saarma, M., Permi, P.
J. Biol. Chem. (2011), 286, 2675-2680, PubMed 21047780 , DOI 10.1074/jbc.M110.146738 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 16776 released on 2010-03-11
Title C-terminal domain of mesencephalic astrocyte-derived neurotrophic factor (MANF)

Related entities 1. MANF, : 1 : 3 : 30 entities Detail

Interaction partners 1. MANF, : 6 interactors Detail

More details for 6 interactors identified by 5 citations

Experiments performed 16 experiments Detail

1 3D 1H-15N NOESY

Instrument

Varian Unity Inova - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 308 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	MANF	[U-98% 13C; U-98% 15N]		1 mM
2	H2O	natural abundance		93 %
3	D2O	natural abundance		7 %

2 3D 1H-13C NOESY

Instrument

Varian Unity Inova - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 308 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	MANF	[U-98% 13C; U-98% 15N]		1 mM
2	H2O	natural abundance		93 %
3	D2O	natural abundance		7 %

3 3D HNCACB

Instrument

Varian Unity Inova - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 308 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	MANF	[U-98% 13C; U-98% 15N]		1 mM
2	H2O	natural abundance		93 %
3	D2O	natural abundance		7 %

4 3D CBCA(CO)NH

Instrument

Varian Unity Inova - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 308 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	MANF	[U-98% 13C; U-98% 15N]		1 mM
2	H2O	natural abundance		93 %
3	D2O	natural abundance		7 %

5 3D HNCA

Instrument

Varian Unity Inova - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 308 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	MANF	[U-98% 13C; U-98% 15N]		1 mM
2	H2O	natural abundance		93 %
3	D2O	natural abundance		7 %

6 3D HN(CO)CA

Instrument

Varian Unity Inova - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 308 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	MANF	[U-98% 13C; U-98% 15N]		1 mM
2	H2O	natural abundance		93 %
3	D2O	natural abundance		7 %

7 3D HCAN

Instrument

Varian Unity Inova - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 308 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	MANF	[U-98% 13C; U-98% 15N]		1 mM
2	H2O	natural abundance		93 %
3	D2O	natural abundance		7 %

8 3D HCA(CO)N

Instrument

Varian Unity Inova - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 308 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	MANF	[U-98% 13C; U-98% 15N]		1 mM
2	H2O	natural abundance		93 %
3	D2O	natural abundance		7 %

9 3D HCCH-TOCSY

Instrument

Varian Unity Inova - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 308 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	MANF	[U-98% 13C; U-98% 15N]		1 mM
2	H2O	natural abundance		93 %
3	D2O	natural abundance		7 %

10 3D HCCH-COSY

Instrument

Varian Unity Inova - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 308 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	MANF	[U-98% 13C; U-98% 15N]		1 mM
2	H2O	natural abundance		93 %
3	D2O	natural abundance		7 %

11 3D H(CCO)NH

Instrument

Varian Unity Inova - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 308 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	MANF	[U-98% 13C; U-98% 15N]		1 mM
2	H2O	natural abundance		93 %
3	D2O	natural abundance		7 %

12 3D C(CO)NH

Instrument

Varian Unity Inova - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 308 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	MANF	[U-98% 13C; U-98% 15N]		1 mM
2	H2O	natural abundance		93 %
3	D2O	natural abundance		7 %

13 2D 1H-15N HSQC

Instrument

Varian Unity Inova - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 308 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	MANF	[U-98% 13C; U-98% 15N]		1 mM
2	H2O	natural abundance		93 %
3	D2O	natural abundance		7 %

14 2D 1H-13C HSQC

Instrument

Varian Unity Inova - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 308 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	MANF	[U-98% 13C; U-98% 15N]		1 mM
2	H2O	natural abundance		93 %
3	D2O	natural abundance		7 %

15 2D (HB)CB(CGCD)HD

Instrument

Varian Unity Inova - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 308 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	MANF	[U-98% 13C; U-98% 15N]		1 mM
2	H2O	natural abundance		93 %
3	D2O	natural abundance		7 %

16 2D (HB)CB(CGCDCE)HE

Instrument

Varian Unity Inova - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 308 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	MANF	[U-98% 13C; U-98% 15N]		1 mM
2	H2O	natural abundance		93 %
3	D2O	natural abundance		7 %

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr16775_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr16775_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	1847		98.1 (chain: 1, length: 160)
1	Chemical shift references	chemical_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 160, Sequence coverage: 98.1%
- Total number of assignments
  - ¹H chemical shifts: 1119
  - ¹³C chemical shifts: 568
  - ¹⁵N chemical shifts: 160
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Keywords NMR, Parkinson's disease, Solution structure, anti-apoptotic, apoptosis, neurotrophic factor, protein