BMRBj - BMREntryMaster

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BMRB: 16776

2KVE

C-terminal domain of mesencephalic astrocyte-derived neurotrophic factor (MANF)

Authors

Hellman, M.H., Saarma, M., Permi, P.

Assembly

C-MANF

Entity

1. C-MANF (polymer, Thiol state: all disulfide bound), 65 monomers, 7461.656 Da Detail

MGKYDKQIDL STVDLKKLRV KELKKILDDW GETCKGCAEK SDYIRKINEL MPKYAPKAAS ARTDL

Formula weight

7461.656 Da

Source organism

Exptl. method

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 83.7 %, Completeness (bb): 77.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	83.7 % (662 of 791)	90.2 % (377 of 418)	73.7 % (224 of 304)	88.4 % (61 of 69)
Backbone	77.7 % (300 of 386)	91.6 % (120 of 131)	63.5 % (122 of 192)	92.1 % (58 of 63)
Sidechain	90.4 % (422 of 467)	89.5 % (257 of 287)	93.1 % (162 of 174)	50.0 % (3 of 6)
Aromatic	94.4 % (34 of 36)	94.4 % (17 of 18)	94.1 % (16 of 17)	100.0 % (1 of 1)
Methyl	100.0 % (68 of 68)	100.0 % (34 of 34)	100.0 % (34 of 34)

1. C-MANF

MGKYDKQIDL STVDLKKLRV KELKKILDDW GETCKGCAEK SDYIRKINEL MPKYAPKAAS ARTDL

Show sample condition

Sample

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	C-MANF	[U-99% 13C; U-99% 15N]		1 mM
2	H2O	natural abundance		93 %
3	D2O	natural abundance		7 %

Release date

2010-03-11

Citation

Mesencephalic astrocyte-derived neurotrophic factor (MANF) has a unique mechanism to rescue apoptotic neurons
Hellman, M.H., Arumae, U., Yu, L., Lindholm, P., Peranen, J., Saarma, M., Permi, P.
J. Biol. Chem. (2011), 286, 2675-2680, PubMed 21047780 , DOI 10.1074/jbc.M110.146738 , Abstract

Related entities 1. C-MANF, : 1 : 10 : 32 entities Detail

Interaction partners 1. C-MANF, : 6 interactors Detail

More details for 6 interactors identified by 5 citations

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC

Instrument

Varian Unity Inova - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	C-MANF	[U-99% 13C; U-99% 15N]		1 mM
2	H2O	natural abundance		93 %
3	D2O	natural abundance		7 %

2 2D 1H-13C HSQC

Instrument

Varian Unity Inova - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	C-MANF	[U-99% 13C; U-99% 15N]		1 mM
2	H2O	natural abundance		93 %
3	D2O	natural abundance		7 %

3 2D 1H-13C-CT-HSQC

Instrument

Varian Unity Inova - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	C-MANF	[U-99% 13C; U-99% 15N]		1 mM
2	H2O	natural abundance		93 %
3	D2O	natural abundance		7 %

4 3D CBCA(CO)NH

Instrument

Varian Unity Inova - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	C-MANF	[U-99% 13C; U-99% 15N]		1 mM
2	H2O	natural abundance		93 %
3	D2O	natural abundance		7 %

5 3D HNCACB

Instrument

Varian Unity Inova - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	C-MANF	[U-99% 13C; U-99% 15N]		1 mM
2	H2O	natural abundance		93 %
3	D2O	natural abundance		7 %

6 3D C(CO)NH

Instrument

Varian Unity Inova - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	C-MANF	[U-99% 13C; U-99% 15N]		1 mM
2	H2O	natural abundance		93 %
3	D2O	natural abundance		7 %

7 3D H(CCO)NH

Instrument

Varian Unity Inova - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	C-MANF	[U-99% 13C; U-99% 15N]		1 mM
2	H2O	natural abundance		93 %
3	D2O	natural abundance		7 %

8 2D (HB)CB(CGCD)HD

Instrument

Varian Unity Inova - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	C-MANF	[U-99% 13C; U-99% 15N]		1 mM
2	H2O	natural abundance		93 %
3	D2O	natural abundance		7 %

9 2D (HB)CB(CGCDCE)HE

Instrument

Varian Unity Inova - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	C-MANF	[U-99% 13C; U-99% 15N]		1 mM
2	H2O	natural abundance		93 %
3	D2O	natural abundance		7 %

10 3D 1H-15N NOESY

Instrument

Varian Unity Inova - 800 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	C-MANF	[U-99% 13C; U-99% 15N]		1 mM
2	H2O	natural abundance		93 %
3	D2O	natural abundance		7 %

11 3D 1H-13C NOESY

Instrument

Varian Unity Inova - 800 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	C-MANF	[U-99% 13C; U-99% 15N]		1 mM
2	H2O	natural abundance		93 %
3	D2O	natural abundance		7 %

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr16776_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr16776_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	749		100.0 (chain: 1, length: 65)
1	Chemical shift references	chemical_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 65, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 461
  - ¹³C chemical shifts: 227
  - ¹⁵N chemical shifts: 61
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Keywords protein