BMRB: 16778

Solution structure of the CBX7 chromodomain in complex with a H3K27me2 peptide

Authors

Yap, K.L., Zeng, L., Zhou, M.

Assembly

CBX7/H3K27me2 complex

Entity

1. CBX7 chromodomain (polymer, Thiol state: not present), 71 monomers, 8538.724 Da Detail

MELSAIGEQV FAVESIRKKR VRKGKVEYLV KWKGWPPKYS TWEPEEHILD PRLVMAYEEK EERDRASGYR K

2. H3K27me2 peptide (polymer, Thiol state: not present), 16 monomers, 1722.042 Da Detail

APRKQLATKA ARXSAP

Total weight

10260.766 Da

Max. entity weight

8538.724 Da

Exptl. method

solution NMR

Refine. method

simulated annealing, torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.9 %, Completeness: 86.2 %, Completeness (bb): 80.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	86.2 % (915 of 1062)	94.0 % (533 of 567)	76.1 % (312 of 410)	82.4 % (70 of 85)
Backbone	80.4 % (405 of 504)	97.6 % (166 of 170)	68.1 % (173 of 254)	82.5 % (66 of 80)
Sidechain	92.3 % (591 of 640)	92.4 % (367 of 397)	92.4 % (220 of 238)	80.0 % (4 of 5)
Aromatic	89.0 % (73 of 82)	100.0 % (41 of 41)	76.3 % (29 of 38)	100.0 % (3 of 3)
Methyl	100.0 % (78 of 78)	100.0 % (39 of 39)	100.0 % (39 of 39)

1. CBX7 chromodomain

MELSAIGEQV FAVESIRKKR VRKGKVEYLV KWKGWPPKYS TWEPEEHILD PRLVMAYEEK EERDRASGYR K

2. H3K27me2 peptide

APRKQLATKA ARXSAP

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4

#	Name	Isotope labeling	Type	Concentration
1	CBX7 chromodomain	[U-100% 15N]		0.5 mM
2	H3K27me2 peptide	natural abundance		0.75 mM
3	sodium phosphate	natural abundance		4.3 mM
4	potassium phosphate	natural abundance		1.4 mM
5	DTT	natural abundance		5 mM
6	sodium chloride	natural abundance		137 mM
7	potassium chloride	natural abundance		3 mM
8	H2O	natural abundance		90 %
9	D2O	natural abundance		10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4

#	Name	Isotope labeling	Type	Concentration
10	CBX7 chromodomain	[U-100% 13C; U-100% 15N]		0.5 mM
11	H3K27me2 peptide	natural abundance		0.75 mM
12	sodium phosphate	natural abundance		4.3 mM
13	potassium phosphate	natural abundance		1.4 mM
14	DTT	natural abundance		5 mM
15	sodium chloride	natural abundance		137 mM
16	potassium chloride	natural abundance		3 mM
17	H2O	natural abundance		90 %
18	D2O	natural abundance		10 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4

#	Name	Isotope labeling	Type	Concentration
19	H3K27me2 peptide	natural abundance		0.5 mM
20	sodium phosphate	natural abundance		4.3 mM
21	potassium phosphate	natural abundance		1.4 mM
22	DTT	natural abundance		5 mM
23	sodium chloride	natural abundance		137 mM
24	potassium chloride	natural abundance		3 mM
25	H2O	natural abundance		90 %
26	D2O	natural abundance		10 %

Hide sample codition

Protein Blocks Logo

Calculated from 20 models in PDB: 2KVM, Strand ID: A, B Detail

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Release date

2011-05-25

Citation

Molecular interplay of the noncoding RNA ANRIL and methylated histone H3 lysine 27 by polycomb CBX7 in transcriptional silencing of INK4a
Yap, K.L., Li, S., Munoz-Cabello, A.M., Raguz, S., Zeng, L., Mujtaba, S., Gil, J., Walsh, M.J., Zhou, M.
Mol. Cell (2010), 38, 662-674, PubMed 20541999 , DOI 10.1016/j.molcel.2010.03.021 , Abstract

Related entities 1. CBX7 chromodomain, : 2 : 1 : 35 entities Detail

Related entities 2. H3K27me2 peptide, : 84 : 34 entities Detail

Interaction partners 2. H3K27me2 peptide, : 1 interactors Detail

Experiments performed 8 experiments Detail