BMRB: 16779

Backbone 1H, 13C, and 15N Chemical Shift Assignments for LMO2(LIM2)-Ldb1(LID)

Authors

Wilkinson-White, L., Dastmalchi, S., Kwan, A., Ryan, D., Mackay, J.P., Matthews, J.

Assembly

Lmo2-LIM2/Ldb1-LID

Entity

1. Lmo2-LIM2/Ldb1-LID (polymer, Thiol state: all free), 123 monomers, 13646.16 Da Detail

YLRLFGQDGL CASCDKRIRA YEMTMRVKDK VYHLECFKCA ACQKHFSVGD RYLLINSDIV CEQDIYEWTK INGGSGGSGG SGGDVMVVGE PTLMGGEFGD EDERLITRLE NTQFDAANGI DDE

Formula weight

13646.16 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.3 %, Completeness: 84.8 %, Completeness (bb): 91.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	84.8 % (1162 of 1371)	85.2 % (605 of 710)	82.8 % (439 of 530)	90.1 % (118 of 131)
Backbone	91.6 % (674 of 736)	89.3 % (233 of 261)	93.8 % (331 of 353)	90.2 % (110 of 122)
Sidechain	79.9 % (593 of 742)	82.9 % (372 of 449)	74.6 % (212 of 284)	100.0 % (9 of 9)
Aromatic	39.1 % (43 of 110)	69.1 % (38 of 55)	7.4 % (4 of 54)	100.0 % (1 of 1)
Methyl	88.6 % (101 of 114)	89.5 % (51 of 57)	87.7 % (50 of 57)

1. Flic

YLRLFGQDGL CASCDKRIRA YEMTMRVKDK VYHLECFKCA ACQKHFSVGD RYLLINSDIV CEQDIYEWTK INGGSGGSGG SGGDVMVVGE PTLMGGEFGD EDERLITRLE NTQFDAANGI DDE

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Temperature 298 K, pH 6.5, Details 15N/13C Lmo2-LIM2/Ldb1-LID (Flic) 865 uM pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	Flic	[U-100% 13C; U-100% 15N]		865 uM
2	DSS	natural abundance		17 uM
3	D2O	natural abundance		5 %
4	MES	natural abundance		20 mM
5	DTT	natural abundance		1 mM
6	H2O	natural abundance		95 %
7	NaCl	natural abundance		150 mM

Sample #2

Solvent system 95% H2O/5% D2O, Temperature 298 K, pH 6.5, Details Unlabeled Lmo2-LIM2/Ldb1-LID (Flic) 630 uM pH 6.5

#	Name	Isotope labeling	Type	Concentration
8	Flic	natural abundance		630 uM
9	DSS	natural abundance		17 uM
10	DTT	natural abundance		1 mM
11	D2O	natural abundance		5 %
12	MES	natural abundance		20 mM
13	H2O	natural abundance		95 %
14	NaCl	natural abundance		150 mM

Sample #3

Solvent system 100% D2O, Temperature 298 K, pH 6.5, Details 15N/13C Lmo2-LIM2/Ldb1-LID (Flic) 865 uM pH 6.5

#	Name	Isotope labeling	Type	Concentration
15	Flic	[U-100% 13C; U-100% 15N]		865 uM
16	DSS	natural abundance		17 uM
17	D2O	natural abundance		100 %
18	MES	natural abundance		20 mM
19	DTT	natural abundance		1 mM
20	NaCl	natural abundance		150 mM

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LACS Plot; CA

Referencing offset: -0.22 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0

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LACS Plot; CB

Referencing offset: -0.22 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0

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LACS Plot; HA

Referencing offset: 0.03 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0

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LACS Plot; CO

Referencing offset: 0.19 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0

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Protein Blocks Logo

Calculated from 20 models in PDB: 2LXD, Strand ID: A Detail

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Release date

2010-10-05

Citation

1H, 15N and 13C assignments of an intramolecular Lmo2-LIM2/Ldb1-LID complex
Wilkinson-White, L.E., Dastmalchi, S., Kwan, A.H., Ryan, D.P., Mackay, J.P., Matthews, J.M.
Biomol. NMR Assign. (2010), 4, 203-206, PubMed 20563763 , DOI 10.1007/s12104-010-9240-y , Abstract

Related entities 1. Lmo2-LIM2/Ldb1-LID, : 1 : 10 entities Detail

Experiments performed 14 experiments Detail