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BMRB: 16788

2KVQ

Solution structure of NusE:NusG-CTD complex

Authors

Burmann, B.M., Schweimer, K., Roesch, P.

Assembly

NusG:NusE

Entity

1. NusE (polymer, Thiol state: not present), 87 monomers, 9593.020 Da Detail

GPLGSMQNQR IRIRLKAFDH RLIDQATAEI VETAKRTGAQ VRGPIPLPTR SRTHLRLVDI VEPTEKTVDA LMRLDLAAGV DVQISLG

2. NusG-CTD (polymer, Thiol state: not present), 63 monomers, 6947.766 Da Detail

GAMGRPKTLF EPGEMVRVND GPFADFNGVV EEVDYEKSRL KVSVSIFGRA TPVELDFSQV EKA

Total weight

16540.785 Da

Max. entity weight

9593.02 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.7 %, Completeness: 74.9 %, Completeness (bb): 74.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	74.9 % (1287 of 1718)	82.7 % (741 of 896)	62.9 % (423 of 672)	82.0 % (123 of 150)
Backbone	74.3 % (655 of 882)	86.8 % (263 of 303)	62.1 % (272 of 438)	85.1 % (120 of 141)
Sidechain	77.8 % (758 of 974)	80.6 % (478 of 593)	74.5 % (277 of 372)	33.3 % (3 of 9)
Aromatic	30.3 % (23 of 76)	60.5 % (23 of 38)	0.0 % (0 of 38)
Methyl	82.6 % (157 of 190)	91.6 % (87 of 95)	73.7 % (70 of 95)

1. NusE

GPLGSMQNQR IRIRLKAFDH RLIDQATAEI VETAKRTGAQ VRGPIPLPTR SRTHLRLVDI VEPTEKTVDA LMRLDLAAGV DVQISLG

2. NusG-CTD

GAMGRPKTLF EPGEMVRVND GPFADFNGVV EEVDYEKSRL KVSVSIFGRA TPVELDFSQV EKA

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Temperature 310 K, pH 7.5

#	Name	Isotope labeling	Concentration
1	15N, 13C -NusG-CTD	[U-100% 13C; U-100% 15N]	300 uM
2	NusE	natural abundance	300 uM
3	NusB	natural abundance	300 uM
4	H2O	natural abundance	90 %
5	D2O	natural abundance	10 %
6	Sodium Chloride	natural abundance	50 mM
7	HEPES	natural abundance	25 mM

Sample #2

Solvent system 90% H2O/10% D2O, Temperature 310 K, pH 7.5

#	Name	Isotope labeling	Concentration
8	15N, 13C, 2H-NusE	[U-100% 13C; U-100% 15N; U-80% 2H]	300 uM
9	NusG-CTD	natural abundance	300 uM
10	NusB	natural abundance	300 uM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %
13	Sodium Chloride	natural abundance	50 mM
14	HEPES	natural abundance	25 mM

Sample #3

Solvent system 90% H2O/10% D2O, Temperature 310 K, pH 7.5

#	Name	Isotope labeling	Concentration
15	NusG-CTD	natural abundance	0.3 mM
16	15N, 13C NusE	[U-100% 13C; U-100% 15N]	0.3 mM
17	NusB	natural abundance	0.3 mM
18	H2O	natural abundance	90 %
19	D2O	natural abundance	10 %
20	Sodium Chloride	natural abundance	50 mM
21	HEPES	natural abundance	25 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.09 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.09 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.14 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 18 models in PDB: 2KVQ, Strand ID: E, G Detail

Release date

2011-05-01

Citation

A NusE:NusG complex links transcription and translation
Burmann, B.M., Schweimer, K., Luo, X., Wahl, M.C., Stitt, B.L., Gottesman, M.E., Roesch, P.
Science (2010), 328, 501-504, PubMed 20413501 , DOI 10.1126/science.1184953 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: P0AFG0 released on 2005-12-20
Title NUSG_ECOLI Entity Transcription termination/antitermination protein NusG

Related entities 1. NusE, : 401 entities Detail

Related entities 2. NusG-CTD, : 1 : 54 : 25 entities Detail

Interaction partners 1. NusE, : 133 interactors Detail

More details for 133 interactors identified by 4 citations

Experiments performed 14 experiments Detail

1 2D 1H-15N HSQC