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BMRB: 16789

2KVR

Solution NMR structure of human ubiquitin specific protease Usp7 UBL domain (residues 537-664). NESG target hr4395c/ SGC-Toronto

Authors

Bezsonova, I., Lemak, A., Avvakumov, G., Bezsonova, I., Dhe-Paganon, S., Arrowsmith, C.

Assembly

usp7-ubl1

Entity

1. usp7-ubl1 (polymer, Thiol state: all free), 130 monomers, 15165.87 Da Detail

GSPQQLVERL QEEKRIEAQK RKERQEAHLY MQVQIVAEDQ FCGHQGNDMY DEEKVKYTVF KVLKNSSLAE FVQSLSQTMG FPQDQIRLWP MQARSNGTKR PAMLDNEADG NKTMIELSDN ENPWTIFLET

Formula weight

15165.87 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.5 %, Completeness: 89.8 %, Completeness (bb): 92.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	89.8 % (1411 of 1571)	89.8 % (747 of 832)	90.4 % (534 of 591)	87.8 % (130 of 148)
Backbone	92.2 % (710 of 770)	91.6 % (239 of 261)	92.7 % (356 of 384)	92.0 % (115 of 125)
Sidechain	89.0 % (823 of 925)	89.0 % (508 of 571)	90.6 % (300 of 331)	65.2 % (15 of 23)
Aromatic	71.7 % (76 of 106)	77.4 % (41 of 53)	64.7 % (33 of 51)	100.0 % (2 of 2)
Methyl	100.0 % (114 of 114)	100.0 % (57 of 57)	100.0 % (57 of 57)

1. usp7-ubl1

GSPQQLVERL QEEKRIEAQK RKERQEAHLY MQVQIVAEDQ FCGHQGNDMY DEEKVKYTVF KVLKNSSLAE FVQSLSQTMG FPQDQIRLWP MQARSNGTKR PAMLDNEADG NKTMIELSDN ENPWTIFLET

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	entity	[U-100% 13C; U-100% 15N]	0.8-1.2 mM
2	sodium phosphate, pH 7.0	natural abundance	20 mM
3	sodium chloride	natural abundance	250 mM
4	DTT	natural abundance	2 mM
5	PMSF	natural abundance	0.5 mM
6	benzamidine	natural abundance	1 mM
7	TCEP	natural abundance	1 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbons	na	indirect	0.2514
¹H	DSS	methyl carbons	internal	direct	1.0
¹⁵N	DSS	methyl carbons	na	indirect	0.1013

Referencing offset: -0.09 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbons	na	indirect	0.2514
¹H	DSS	methyl carbons	internal	direct	1.0
¹⁵N	DSS	methyl carbons	na	indirect	0.1013

Referencing offset: -0.09 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbons	na	indirect	0.2514
¹H	DSS	methyl carbons	internal	direct	1.0
¹⁵N	DSS	methyl carbons	na	indirect	0.1013

Referencing offset: 0.0 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbons	na	indirect	0.2514
¹H	DSS	methyl carbons	internal	direct	1.0
¹⁵N	DSS	methyl carbons	na	indirect	0.1013

Referencing offset: 0.09 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2KVR, Strand ID: A Detail

Release date

2010-09-23

Citation

Structural Characterization of Interaction between Human Ubiquitin-specific Protease 7 and Immediate-Early Protein ICP0 of Herpes Simplex Virus-1
Bezsonova, I., Avvakumov, G., Dhe-Paganon, S., Arrowsmith, C.
J. Biol. Chem. (2015), 290, 22907-22918, PubMed 26224631 , DOI 10.1074/jbc.M115.664805 , Abstract

Related entities 1. usp7-ubl1, : 1 : 1 : 12 : 11 entities Detail

Interaction partners 1. usp7-ubl1, : 98 interactors Detail

More details for 98 interactors identified by 47 citations

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC