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BMRB: 16790

2X8N

Solution NMR structure of uncharacterized protein CV0863 from Chromobacterium violaceum. NESG target CvT3. OCSP target CV0863.

Authors

Gutmanas, A., Fares, C., Yee, A., Lemak, A., Semesi, A., Arrowsmith, C.H.

Assembly

CV0863

Entity

1. CV0863 (polymer, Thiol state: not present), 111 monomers, 12495.86 Da Detail

QGMEVSANEL EAASSRMEML QREYSTLRSV QYRSEEGVIV FILANDRELK FRPDDLQATY GATPEQLREI EISPSGLGVY FETLEEDVSL IGLLEGRRGS AKWMAEHPLA S

Formula weight

12495.86 Da

Source organism

Chromobacterium violaceum ATCC 12472

Exptl. method

solution NMR

Refine. method

TORSION ANGLE DYNAMICS, RESTRAINED MOLECULAR DYNAMICS

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 98.2 %, Completeness: 95.4 %, Completeness (bb): 95.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	95.4 % (1214 of 1272)	94.1 % (624 of 663)	97.2 % (480 of 494)	95.7 % (110 of 115)
Backbone	95.6 % (629 of 658)	94.2 % (213 of 226)	96.3 % (313 of 325)	96.3 % (103 of 107)
Sidechain	95.8 % (687 of 717)	94.1 % (411 of 437)	98.9 % (269 of 272)	87.5 % (7 of 8)
Aromatic	100.0 % (78 of 78)	100.0 % (39 of 39)	100.0 % (38 of 38)	100.0 % (1 of 1)
Methyl	100.0 % (122 of 122)	100.0 % (61 of 61)	100.0 % (61 of 61)

1. CV0863

QGMEVSANEL EAASSRMEML QREYSTLRSV QYRSEEGVIV FILANDRELK FRPDDLQATY GATPEQLREI EISPSGLGVY FETLEEDVSL IGLLEGRRGS AKWMAEHPLA S

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1.0 atm, Temperature 298.0 K, pH 7.0, Details 100%13C,100%15N-CV0863 1mM

#	Name	Isotope labeling	Concentration
1	CV0863	[U-100% 13C; U-100% 15N]	1.0 mM
2	TRIS	natural abundance	10.0 mM
3	NaCl	natural abundance	300.0 mM
4	ZnSO4	natural abundance	10.0 mM
5	DTT	natural abundance	10.0 mM
6	benzamidine	natural abundance	1.0 mM
7	NaN3	natural abundance	0.01 %
8	Roch inhibitor cocktail	natural abundance	1 na

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1.0 atm, Temperature 298.0 K, pH 7.0, Details 7%13C,100%15N-CV0863 1mM

#	Name	Isotope labeling	Concentration
9	CV0863	[U-7% 13C; U-100% 15N]	1.0 mM
10	TRIS	natural abundance	10.0 mM
11	NaCl	natural abundance	300.0 mM
12	ZnSO4	natural abundance	10.0 mM
13	DTT	natural abundance	10.0 mM
14	benzamidine	natural abundance	1.0 mM
15	NaN3	natural abundance	0.01 %
16	Roche inhibitor cocktail	natural abundance	1 na

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.07 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.07 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.05 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.33 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2X8N, Strand ID: A Detail

Release date

2010-03-30

Related entities 1. CV0863, : 1 : 4 entities Detail

Experiments performed 19 experiments Detail

1 13C-NOESY-HSQC-aliphatic