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Found 1 Document (1.398 seconds)

BMRB: 16820

2KCY

SOLUTION STRUCTURE OF RIBOSOMAL PROTEIN S8E FROM Methanothermobacter thermautotrophicus, NORTHEASTSTRUCTURAL GENOMICS CONSORTIUM (NESG) TARGET TR71D

Authors: Liu, G., Rossi, P., Wang, D., Nwosu, C., OWENS, L., Xiao, R., Liu, J., BARAN, M.C., SWAPNA, G., ACTON, T.B., Rost, B.C., Montelione, G.T.
Assembly: S8E
Entity: 1. S8E (polymer, Thiol state: not present), 294 monomers, 33018.84 Da Detail
GDRRVRLIRT RGGNTKVRLA SDTRINVVDP ETGKVEIAEI RNVVENTANP HFVRRNIITR GAVVETNLGN VRVTSRPGQD GVINGVLIRE LEHHHHHHGD RRVRLIRTRG GNTKVRLASD TRINVVDPET GKVEIAEIRN VVENTANPHF VRRNIITRGA VVETNLGNVR VTSRPGQDGV INGVLIRELE HHHHHHGDRR VRLIRTRGGN TKVRLASDTR INVVDPETGK VEIAEIRNVV ENTANPHFVR RNIITRGAVV ETNLGNVRVT SRPGQDGVIN GVLIRELEHH HHHH
Formula weight: 33018.84 Da
Source organism: Methanothermobacter thermautotrophicus
Exptl. method: solution NMR
Refine. method: distance geometry, simulated annealing, molecular dynamics, torsion angle dynamics
Data set: RDCs
Protein Blocks Logo: Calculated from 20 models in PDB: 2KCY, Strand ID: A Detail
RDC: 45 RDC values in 1 lists
Field strength (¹H) 600 MHz, Pressure 1 atm, Temperature 298 K, pH 6.5 Detail
Pressure 1 atm, Temperature 298 K, pH 6.5
Experiment name 2D 1H-15N HSQC
List #1 RDC_list_1, RDC code DHN, Field strength (¹H) 600 MHz
Release date: 2012-08-02
Citation: SOLUTION STRUCTURE OF RIBOSOMAL PROTEIN S8E FROM Methanothermobacter thermautotrophicus, NORTHEASTSTRUCTURAL GENOMICS CONSORTIUM (NESG) TARGET TR71D
Liu, G., Rossi, P., Montelione, G.T.
Not known

Experiments performed 15 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 800 MHz nysbc

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity	[U-100% 13C; U-100% 15N]	0.94 mM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %

2 2D 1H-15N HSQC

Instrument

GE Avance - 800 MHz cabm

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity	[U-100% 13C; U-100% 15N]	0.94 mM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %

3 2D 1H-15N HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity	[U-100% 13C; U-100% 15N]	0.94 mM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %

4 3D 1H-15N NOESY

Instrument

Bruker Avance - 800 MHz nysbc

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity	[U-100% 13C; U-100% 15N]	0.94 mM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %

5 3D 1H-13C NOESY

Instrument

Bruker Avance - 800 MHz nysbc

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity	[U-100% 13C; U-100% 15N]	0.94 mM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %

6 3D 1H-13C NOESY

Instrument

GE Avance - 800 MHz cabm

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details sample_1 dissolved in D2O

#	Name	Isotope labeling	Type	Concentration
7	entity	[U-100% 13C; U-100% 15N]		0.94 mM
8	D2O	natural abundance		100 %

7 3D HNCO

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity	[U-100% 13C; U-100% 15N]	0.94 mM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %

8 3D CBCA(CO)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity	[U-100% 13C; U-100% 15N]	0.94 mM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %

9 3D HNCACB

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity	[U-100% 13C; U-100% 15N]	0.94 mM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %

10 3D C(CO)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity	[U-100% 13C; U-100% 15N]	0.94 mM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %

11 3D HBHA(CO)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity	[U-100% 13C; U-100% 15N]	0.94 mM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %

12 2D 1H-15N HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
4	entity	[U-10% 13C; U-99% 15N]	1.0 mM
5	H2O	natural abundance	95 %
6	D2O	natural abundance	5 %

13 2D 1H-13C HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
4	entity	[U-10% 13C; U-99% 15N]	1.0 mM
5	H2O	natural abundance	95 %
6	D2O	natural abundance	5 %

14 2D 1H-13C HSQC

Instrument

GE Avance - 800 MHz cabm

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details sample_1 dissolved in D2O

#	Name	Isotope labeling	Type	Concentration
7	entity	[U-100% 13C; U-100% 15N]		0.94 mM
8	D2O	natural abundance		100 %

15 2D 1H-15N HSQC_TROSY

Instrument

Varian INOVA - 600 MHz

Sample

State anisotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details sample_2 aligned in in Peg/hexanol.

#	Name	Isotope labeling	Concentration
9	entity	[U-10% 13C; U-99% 15N]	1.0 mM
10	H2O	natural abundance	95 %
11	D2O	natural abundance	5 %

nullKeywords BETA, NESG, RDC, RIBOSOMAL PROTEIN S8E, rsp8e

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Found 1 Document (1.398 seconds)

BMRB: 16820

Experiments performed 15 experiments Detail

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