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Found 1 Document (1.046 seconds)

BMRB: 16821

2KCV

SOLUTION NMR STRUCTURE OF TETRATRICOPEPTIDE REPEAT DOMAIN PROTEIN SRU_0103 FROM SALINIBACTER RUBER, NORTHEAST STRUCTURAL GENOMICS CONSORTIUM (NESG) TARGET SRR115C

Authors: Liu, G., Rossi, P., Wang, D., Nwosu, C., Owens, L., Xiao, R., Liu, J., Baran, M.C., SWAPNA, G., ACTON, T.B., Rost, B., Montelione, G.T.
Assembly: SRU 0103
Entity: 1. SRU 0103 (polymer, Thiol state: not present), 198 monomers, 22918.51 Da Detail
EDPEDPFTRY ALAQEHLKHD NASRALALFE ELVETDPDYV GTYYHLGKLY ERLDRTDDAI DTYAQGIEVA REEGTQKDLS ELQDAKLKAE GLEHHHHHHE DPEDPFTRYA LAQEHLKHDN ASRALALFEE LVETDPDYVG TYYHLGKLYE RLDRTDDAID TYAQGIEVAR EEGTQKDLSE LQDAKLKAEG LEHHHHHH
Formula weight: 22918.51 Da
Source organism: Salinibacter ruber
Exptl. method: solution NMR
Refine. method: distance geometry, torsion angle dynamics, simulated annealing, molecular dynamics
Data set: RDCs
Protein Blocks Logo: Calculated from 20 models in PDB: 2KCV, Strand ID: A Detail
RDC: 70 RDC values in 1 lists
Field strength (¹H) 600 MHz, Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.5 Detail
Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.5
Experiment name 2D 1H-15N HSQC
List #1 RDC_list_1, RDC code DHN, Field strength (¹H) 600 MHz
Release date: 2012-08-02
Citation: SOLUTION NMR STRUCTURE OF TETRATRICOPEPTIDE REPEAT DOMAIN PROTEIN SRU_0103 FROM SALINIBACTER RUBER, NORTHEAST STRUCTURAL GENOMICS CONSORTIUM (NESG) TARGET SRR115C
Liu, G., Montelione, G.
Not known

Related entities 1. SRU 0103, : 85 entities Detail

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity	[U-100% 13C; U-100% 15N]	1.37 mM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %

2 2D 1H-15N HSQC

Instrument

Varian INOVA - 600 MHz in CABM

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity	[U-100% 13C; U-100% 15N]	1.37 mM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %

3 2D 1H-15N HSQC-TROSY

Instrument

Varian INOVA - 600 MHz RDC

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.5, Details for RDC data collection

#	Name	Isotope labeling	Concentration
7	entity	[U-10% 13C; U-99% 15N]	1.09 mM
8	H2O	natural abundance	95 %
9	D2O	natural abundance	5 %

4 3D 1H-15N NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity	[U-100% 13C; U-100% 15N]	1.37 mM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %

5 3D 1H-13C NOESY-aliphatic

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity	[U-100% 13C; U-100% 15N]	1.37 mM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %

6 3D 1H-13C NOESY-aromatic

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity	[U-100% 13C; U-100% 15N]	1.37 mM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %

7 4,3D GFT CABCACONHN

Instrument

Varian INOVA - 600 MHz in CABM

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity	[U-100% 13C; U-100% 15N]	1.37 mM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %

8 4,3D GFT HNNCABCA

Instrument

Varian INOVA - 600 MHz in CABM

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity	[U-100% 13C; U-100% 15N]	1.37 mM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %

9 4,3D GFT HABCABCONHN

Instrument

Varian INOVA - 600 MHz in CABM

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity	[U-100% 13C; U-100% 15N]	1.37 mM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %

10 3D HCCH-COSY

Instrument

Varian INOVA - 600 MHz in CABM

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity	[U-100% 13C; U-100% 15N]	1.37 mM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %

11 2D 1H-15N HSQC

Instrument

Varian INOVA - 600 MHz in CABM

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.5

#	Name	Isotope labeling	Concentration
4	entity	[U-10% 13C; U-99% 15N]	1.05 mM
5	H2O	natural abundance	95 %
6	D2O	natural abundance	5 %

12 2D 1H-13C HSQC

Instrument

Varian INOVA - 600 MHz in CABM

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.5

#	Name	Isotope labeling	Concentration
4	entity	[U-10% 13C; U-99% 15N]	1.05 mM
5	H2O	natural abundance	95 %
6	D2O	natural abundance	5 %

nullKeywords GFT NMR, NESG, RDC, TETRATRICOPEPTIDE REPEAT, TPR

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Found 1 Document (1.046 seconds)

BMRB: 16821

Related entities 1. SRU 0103, : 85 entities Detail

Experiments performed 12 experiments Detail

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