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BMRB: 16832

2KTL

Structure of C-terminal domain from mtTyrRS of A. nidulans

Authors

Chari, N.S.

Assembly

C-terminal domain from mtTyrRS

Entity

1. C-terminal domain from mtTyrRS (polymer, Thiol state: not present), 164 monomers, 17652.96 Da Detail

MPRTASPGNP KSSLSGFVNP QSGNPHAPQT NFANMPSARV TLPKSLVYDK TFSKVLWSAG LVASKSEGQR IINNNGAYVG SRPGVKKNEP GGGMPDDLTF TPIKTWNASK TQEFIIDGDL LILKLGKWKM KLVSIVSDEK FKELGLTAPG WDEVVGKGKE EPSP

Formula weight

17652.96 Da

Source organism

Aspergillus nidulans

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.9 %, Completeness: 71.9 %, Completeness (bb): 87.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	71.9 % (1365 of 1898)	70.0 % (697 of 996)	72.1 % (530 of 735)	82.6 % (138 of 167)
Backbone	87.1 % (831 of 954)	85.5 % (282 of 330)	86.9 % (413 of 475)	91.3 % (136 of 149)
Sidechain	61.0 % (666 of 1091)	62.3 % (415 of 666)	61.2 % (249 of 407)	11.1 % (2 of 18)
Aromatic	21.9 % (28 of 128)	43.8 % (28 of 64)	0.0 % (0 of 60)	0.0 % (0 of 4)
Methyl	78.8 % (126 of 160)	80.0 % (64 of 80)	77.5 % (62 of 80)

1. mtTyrRS

MPRTASPGNP KSSLSGFVNP QSGNPHAPQT NFANMPSARV TLPKSLVYDK TFSKVLWSAG LVASKSEGQR IINNNGAYVG SRPGVKKNEP GGGMPDDLTF TPIKTWNASK TQEFIIDGDL LILKLGKWKM KLVSIVSDEK FKELGLTAPG WDEVVGKGKE EPSP

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	entity	[U-100% 15N]	20 mg
2	entity	[U-100% 13C]	20 mg
3	sodium chloride	natural abundance	500 mM
4	TRIS	natural abundance	10 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4

#	Name	Isotope labeling	Concentration
7	sodium chloride	natural abundance	500 mM
8	TRIS	natural abundance	10 mM
9	H2O	natural abundance	90 %
10	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbon	internal	indirect	0.2514495
¹H	DSS	methyl carbon	internal	direct	1.0
¹⁵N	DSS	methyl carbon	internal	indirect	0.1013291

Referencing offset: -0.19 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbon	internal	indirect	0.2514495
¹H	DSS	methyl carbon	internal	direct	1.0
¹⁵N	DSS	methyl carbon	internal	indirect	0.1013291

Referencing offset: -0.19 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbon	internal	indirect	0.2514495
¹H	DSS	methyl carbon	internal	direct	1.0
¹⁵N	DSS	methyl carbon	internal	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbon	internal	indirect	0.2514495
¹H	DSS	methyl carbon	internal	direct	1.0
¹⁵N	DSS	methyl carbon	internal	indirect	0.1013291

Referencing offset: 1.06 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 12 models in PDB: 2KTL, Strand ID: A Detail

Release date

2012-08-02

Citation

Structural Divergence of the Group I Intron-Binding Surface in Fungal Mitochondrial Tyrosyl-tRNA Synthetases that Function in RNA Splicing
Hoffman, D., Lambowitz, A., Chari, N., Paukstelis, P.
J. Biol. Chem. (2016), PubMed 27036943 , DOI 10.1074/jbc.M116.725390 , Abstract

Related entities 1. C-terminal domain from mtTyrRS, : 1 entities Detail

Experiments performed 16 experiments Detail

1 2D 1H-15N HSQC