BMRBj - BMREntryMaster

Found 1 Document (4.565 seconds)

BMRB: 16843

2K3B

Seeing the Invisible: Structures of Excited Protein States by Relaxation Dispersion NMR

Authors

Vallurupalli, P., Hansen, F.D., Kay, L.E.

Assembly

Abp1p

Entity

1. Abp1p (polymer, Thiol state: not present), 62 monomers, 6990.458 Da Detail

GAMAPWATAE YDYDAAEDNE LTFVENDKII NIEFVDDDWW LGELEKDGSK GLFPSNYVSL GN

2. Ark1p (polymer, Thiol state: not present), 17 monomers, 1909.316 Da Detail

KKTKPTPPPK PSHLKPK

Total weight

8899.774 Da

Max. entity weight

6990.458 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 74.7 %, Completeness: 25.6 %, Completeness (bb): 48.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	25.6 % (238 of 931)	15.4 % (75 of 487)	28.8 % (105 of 365)	73.4 % (58 of 79)
Backbone	48.3 % (221 of 458)	38.1 % (59 of 155)	45.3 % (105 of 232)	80.3 % (57 of 71)
Sidechain	4.0 % (22 of 547)	4.8 % (16 of 332)	2.4 % (5 of 207)	12.5 % (1 of 8)
Aromatic	0.0 % (0 of 94)	0.0 % (0 of 47)	0.0 % (0 of 44)	0.0 % (0 of 3)
Methyl	5.9 % (4 of 68)	8.8 % (3 of 34)	2.9 % (1 of 34)

1. Abp1p

GAMAPWATAE YDYDAAEDNE LTFVENDKII NIEFVDDDWW LGELEKDGSK GLFPSNYVSL GN

2. Ark1p

KKTKPTPPPK PSHLKPK

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 50 mM Sodium Phosphate, 100 mM NaCl, 1mM EDTA, 1mM NaN3, pH 7.0 Aligned using Pf1 phage

#	Name	Isotope labeling	Concentration
1	Abp1p	[U-90% 2H; U-100% 15N]	1.5 mM
2	Ark1p	[U-90% 2H; U-100% 15N]	0.105 mM
3	Sodium Phosphate	natural abundance	50 mM
4	NaCl	natural abundance	100 mM
5	EDTA	natural abundance	1 mM
6	NaN3	natural abundance	1 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 50 mM Sodium Phosphate, 100 mM NaCl, 1mM EDTA, 1mM NaN3, pH 7.0 Aligned using PEG (C12E5)/hexanol, D2O Spliting 21 Hz

#	Name	Isotope labeling	Concentration
7	Abp1p	[U-90% 2H; U-100% 15N]	1.5 mM
8	Ark1p	natural abundance	0.105 mM
9	Sodium Phosphate	natural abundance	50 mM
10	NaCl	natural abundance	100 mM
11	EDTA	natural abundance	1 mM
12	NaN3	natural abundance	1 mM

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 50 mM Sodium Phosphate, 100 mM NaCl, 1mM EDTA, 1mM NaN3, pH 7.0 Aligned using pf1 phage

#	Name	Isotope labeling	Concentration
13	Abp1p	[U-90% 2H; U-100% 15N; 13CO labeled]	1.5 mM
14	Ark1p	natural abundance	0.105 mM
15	Sodium Phosphate	natural abundance	50 mM
16	NaCl	natural abundance	100 mM
17	EDTA	natural abundance	1 mM
18	NaN3	natural abundance	1 mM

Sample #4

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 50 mM Sodium Phosphate, 100 mM NaCl, 1mM EDTA, 1mM NaN3, pH 7.0 Aligned using pf1 phage

#	Name	Isotope labeling	Concentration
19	Abp1p	[U-90% 2H; U-100% 15N; 13Ca labeled]	1.5 mM
20	Ark1p	natural abundance	0.105 mM
21	Sodium Phosphate	natural abundance	50 mM
22	NaCl	natural abundance	100 mM
23	EDTA	natural abundance	1 mM
24	NaN3	natural abundance	1 mM

Protein Blocks Logo

Calculated from 10 models in PDB: 2K3B, Strand ID: A Detail

Release date

2012-08-02

Citation

Probing the free energy landscape of the fast-folding gpW protein by relaxation dispersion NMR
Sanchez-Medina, C., Sekhar, A., Vallurupalli, P., Cerminara, M., Munoz, V., Kay, L.E.
J. Am. Chem. Soc. (2014), 136, 7444-7451, PubMed 24805164 , DOI 10.1021/ja502705y , Abstract

Related entities 1. Abp1p, : 2 : 278 entities Detail

Related entities 2. Ark1p, : 3 entities Detail

Interaction partners 1. Abp1p, : 49 : 1 interactors Detail

More details for 50 interactors identified by 11 citations

Interaction partners 2. Ark1p, : 7 : 1 interactors Detail

More details for 8 interactors identified by 7 citations

Experiments performed 8 experiments Detail

1 15N CT-CW,TROSY,anti-TROSY CPMG

Instrument

Varian INOVA - 500 MHz

Sample

State anisotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 50 mM Sodium Phosphate, 100 mM NaCl, 1mM EDTA, 1mM NaN3, pH 7.0 Aligned using Pf1 phage

#	Name	Isotope labeling	Concentration
1	Abp1p	[U-90% 2H; U-100% 15N]	1.5 mM
2	Ark1p	[U-90% 2H; U-100% 15N]	0.105 mM
3	Sodium Phosphate	natural abundance	50 mM
4	NaCl	natural abundance	100 mM
5	EDTA	natural abundance	1 mM
6	NaN3	natural abundance	1 mM

2 15N CT-CW,TROSY,anti-TROSY CPMG

Instrument

Varian INOVA - 500 MHz

Sample

#	Name	Isotope labeling	Concentration
7	Abp1p	[U-90% 2H; U-100% 15N]	1.5 mM
8	Ark1p	natural abundance	0.105 mM
9	Sodium Phosphate	natural abundance	50 mM
10	NaCl	natural abundance	100 mM
11	EDTA	natural abundance	1 mM
12	NaN3	natural abundance	1 mM

3 13CO CT-inphase,TROSY,anti-TROSY CPMG

Instrument

Varian INOVA - 500 MHz

Sample

#	Name	Isotope labeling	Concentration
13	Abp1p	[U-90% 2H; U-100% 15N; 13CO labeled]	1.5 mM
14	Ark1p	natural abundance	0.105 mM
15	Sodium Phosphate	natural abundance	50 mM
16	NaCl	natural abundance	100 mM
17	EDTA	natural abundance	1 mM
18	NaN3	natural abundance	1 mM

4 13CA CT-CW,TROSY,anti-TROSY CPMG

Instrument

Varian INOVA - 500 MHz

Sample

State anisotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 50 mM Sodium Phosphate, 100 mM NaCl, 1mM EDTA, 1mM NaN3, pH 7.0 Aligned using pf1 phage

#	Name	Isotope labeling	Concentration
19	Abp1p	[U-90% 2H; U-100% 15N; 13Ca labeled]	1.5 mM
20	Ark1p	natural abundance	0.105 mM
21	Sodium Phosphate	natural abundance	50 mM
22	NaCl	natural abundance	100 mM
23	EDTA	natural abundance	1 mM
24	NaN3	natural abundance	1 mM

5 15N CT-CW CPMG

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 50 mM Sodium Phosphate, 100 mM NaCl, 1mM EDTA, 1mM NaN3, pH 7.0 Aligned using PEG (C12E5)/hexanol, D2O Spliting 21 Hz

#	Name	Isotope labeling	Concentration
7	Abp1p	[U-90% 2H; U-100% 15N]	1.5 mM
8	Ark1p	natural abundance	0.105 mM
9	Sodium Phosphate	natural abundance	50 mM
10	NaCl	natural abundance	100 mM
11	EDTA	natural abundance	1 mM
12	NaN3	natural abundance	1 mM

6 13 CO CT CPMG

Instrument

Varian INOVA - 500 MHz

Sample

#	Name	Isotope labeling	Concentration
13	Abp1p	[U-90% 2H; U-100% 15N; 13CO labeled]	1.5 mM
14	Ark1p	natural abundance	0.105 mM
15	Sodium Phosphate	natural abundance	50 mM
16	NaCl	natural abundance	100 mM
17	EDTA	natural abundance	1 mM
18	NaN3	natural abundance	1 mM

7 13 CA CT-CW CPMG

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 50 mM Sodium Phosphate, 100 mM NaCl, 1mM EDTA, 1mM NaN3, pH 7.0 Aligned using pf1 phage

#	Name	Isotope labeling	Concentration
19	Abp1p	[U-90% 2H; U-100% 15N; 13Ca labeled]	1.5 mM
20	Ark1p	natural abundance	0.105 mM
21	Sodium Phosphate	natural abundance	50 mM
22	NaCl	natural abundance	100 mM
23	EDTA	natural abundance	1 mM
24	NaN3	natural abundance	1 mM

8 1H CT-CW CPMG

Instrument

Varian INOVA - 500 MHz

Sample

#	Name	Isotope labeling	Concentration
7	Abp1p	[U-90% 2H; U-100% 15N]	1.5 mM
8	Ark1p	natural abundance	0.105 mM
9	Sodium Phosphate	natural abundance	50 mM
10	NaCl	natural abundance	100 mM
11	EDTA	natural abundance	1 mM
12	NaN3	natural abundance	1 mM

nullKeywords Abp1p, Ark1p, CPMG, Invisible State, NMR

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Found 1 Document (4.565 seconds)

BMRB: 16843

Sample #1

Sample #2

Sample #3

Sample #4

Related entities 1. Abp1p, : 2 : 278 entities Detail

Related entities 2. Ark1p, : 3 entities Detail

Interaction partners 1. Abp1p, : 49 : 1 interactors Detail

Interaction partners 2. Ark1p, : 7 : 1 interactors Detail

Experiments performed 8 experiments Detail

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