BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	92.1 % (1301 of 1412)	90.7 % (653 of 720)	93.1 % (526 of 565)	96.1 % (122 of 127)
Backbone	95.5 % (728 of 762)	95.8 % (248 of 259)	95.1 % (365 of 384)	96.6 % (115 of 119)
Sidechain	89.9 % (694 of 772)	87.9 % (405 of 461)	93.1 % (282 of 303)	87.5 % (7 of 8)
Aromatic	63.3 % (38 of 60)	73.3 % (22 of 30)	51.7 % (15 of 29)	100.0 % (1 of 1)
Methyl	96.1 % (173 of 180)	95.6 % (86 of 90)	96.7 % (87 of 90)

1. Rv0899

GASALSLSLL SISRSGNTVT LIGDFPDEAA KAALMTALNG LLAPGVNVID QIHVDPVVRS LDFSSAEPVF TASVPIPDFG LKVERDTVTL TGTAPSSEHK DAVKRAATST WPDMKIVNNI EVTGQAPPGP P

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 (±0.1) K, pH 7 (±0.05), Details Rv0899(73-220) in 5 mM phosphate, pH 7.0

#	Name	Isotope labeling	Concentration
1	Rv0899(73-220)	[U-99% 15N]	1.7 (±0.1) mM
2	Rv0899(73-220)	[U-99% 13C; U-99% 15N]	1 (±0.1) mM
3	phosphate	natural abundance	5 mM
4	H2O	natural abundance	95 %
5	D2O	natural abundance	5 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 313 (±0.1) K, pH 7 (±0.05), Details Rv0899(73-220) in 5 mM phosphate, pH 7.0

#	Name	Isotope labeling	Concentration
6	Rv0899(73-220)	[U-99% 13C; U-99% 15N]	1 (±0.1) mM
7	Rv0899(73-220)	[U-99% 15N]	0.3 (±0.1) mM
8	D2O	natural abundance	100 %
9	phosphate	natural abundance	5 mM

Sample #3

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 (±0.1) K, pH 7 (±0.05), Details Rv0899(73-220) in 15 mg/ml Pf1 phage, 5 mM phosphate, pH 7.0

#	Name	Isotope labeling	Concentration
10	Rv0899(73-220)	[U-99% 15N]	0.4 (±0.1) mM
11	Pf1 phage	natural abundance	15 mg/mL
12	phosphate	natural abundance	5 mM
13	H2O	natural abundance	95 %
14	D2O	natural abundance	5 %

Sample #4

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 (±0.1) K, pH 7 (±0.05), Details Rv0899(73-220) in 10 mg/ml fd phage, 5 mM phosphate, 200 mM KCl, pH 7.0

#	Name	Isotope labeling	Concentration
15	Rv0899(73-220)	[U-99% 15N]	0.4 (±0.1) mM
16	fd phage	natural abundance	10 mg/mL
17	phosphate	natural abundance	5 mM
18	H2O	natural abundance	95 %
19	D2O	natural abundance	5 %
20	KCl	natural abundance	200 mM

Sample #5

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 (±0.1) K, pH 7 (±0.05), Details Rv0899(73-220) in 4.5% compressed polyacrylamide gel, 5 mM phosphate, pH 7.0

#	Name	Isotope labeling	Concentration
21	Rv0899 73-220	[U-99% 15N]	0.4 (±0.1) mM
22	polyacrylamide gel	natural abundance	4.5 %
23	phosphate	natural abundance	5 mM
24	H2O	natural abundance	95 %
25	D2O	natural abundance	5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹³C	na	na	null	null
¹H	na	na	null	null
¹⁵N	na	na	null	null

Referencing offset: 0.39 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹³C	na	na	null	null
¹H	na	na	null	null
¹⁵N	na	na	null	null

Referencing offset: 0.39 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹³C	na	na	null	null
¹H	na	na	null	null
¹⁵N	na	na	null	null

Referencing offset: -0.12 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹³C	na	na	null	null
¹H	na	na	null	null
¹⁵N	na	na	null	null

Referencing offset: 0.26 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2KSM, Strand ID: A Detail

Release date

2010-05-09

Citation

Mycobacterium tuberculosis Rv0899 adopts a mixed alpha/beta-structure and does not form a transmembrane beta-barrel
Teriete, P., Yao, Y., Kolodzik, A., Yu, J., Song, H.M., Niederweis, M., Marassi, F.M.
Biochemistry (2010), 49, 2768-2777, PubMed 20199110 , DOI 10.1021/bi100158s , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: P9WIU5 released on 2014-04-16
Title ARFA_MYCTU Entity Peptidoglycan-binding protein ArfA

Related entities 1. Rv0899, : 1 : 4 : 1 entities Detail

Experiments performed 13 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 600 MHz with cryoprobe

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 (±0.1) K, pH 7 (±0.05), Details Rv0899(73-220) in 5 mM phosphate, pH 7.0

#	Name	Isotope labeling	Concentration
1	Rv0899(73-220)	[U-99% 15N]	1.7 (±0.1) mM
2	Rv0899(73-220)	[U-99% 13C; U-99% 15N]	1 (±0.1) mM
3	phosphate	natural abundance	5 mM
4	H2O	natural abundance	95 %
5	D2O	natural abundance	5 %

2 IPAP

Instrument

Bruker Avance - 800 MHz

Sample

State anisotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 (±0.1) K, pH 7 (±0.05), Details Rv0899(73-220) in 15 mg/ml Pf1 phage, 5 mM phosphate, pH 7.0

#	Name	Isotope labeling	Concentration
10	Rv0899(73-220)	[U-99% 15N]	0.4 (±0.1) mM
11	Pf1 phage	natural abundance	15 mg/mL
12	phosphate	natural abundance	5 mM
13	H2O	natural abundance	95 %
14	D2O	natural abundance	5 %

3 IPAP

Instrument

Bruker Avance - 800 MHz

Sample

State anisotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 (±0.1) K, pH 7 (±0.05), Details Rv0899(73-220) in 10 mg/ml fd phage, 5 mM phosphate, 200 mM KCl, pH 7.0

#	Name	Isotope labeling	Concentration
15	Rv0899(73-220)	[U-99% 15N]	0.4 (±0.1) mM
16	fd phage	natural abundance	10 mg/mL
17	phosphate	natural abundance	5 mM
18	H2O	natural abundance	95 %
19	D2O	natural abundance	5 %
20	KCl	natural abundance	200 mM

4 IPAP

Instrument

Bruker Avance - 800 MHz

Sample

State anisotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 (±0.1) K, pH 7 (±0.05), Details Rv0899(73-220) in 4.5% compressed polyacrylamide gel, 5 mM phosphate, pH 7.0

#	Name	Isotope labeling	Concentration
21	Rv0899 73-220	[U-99% 15N]	0.4 (±0.1) mM
22	polyacrylamide gel	natural abundance	4.5 %
23	phosphate	natural abundance	5 mM
24	H2O	natural abundance	95 %
25	D2O	natural abundance	5 %

5 2D 1H-13C HSQC

Instrument

Bruker Avance - 600 MHz with cryoprobe

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 (±0.1) K, pH 7 (±0.05), Details Rv0899(73-220) in 5 mM phosphate, pH 7.0

#	Name	Isotope labeling	Concentration
1	Rv0899(73-220)	[U-99% 15N]	1.7 (±0.1) mM
2	Rv0899(73-220)	[U-99% 13C; U-99% 15N]	1 (±0.1) mM
3	phosphate	natural abundance	5 mM
4	H2O	natural abundance	95 %
5	D2O	natural abundance	5 %

6 3D HNCA

Instrument

Bruker Avance - 600 MHz with cryoprobe

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 (±0.1) K, pH 7 (±0.05), Details Rv0899(73-220) in 5 mM phosphate, pH 7.0

#	Name	Isotope labeling	Concentration
1	Rv0899(73-220)	[U-99% 15N]	1.7 (±0.1) mM
2	Rv0899(73-220)	[U-99% 13C; U-99% 15N]	1 (±0.1) mM
3	phosphate	natural abundance	5 mM
4	H2O	natural abundance	95 %
5	D2O	natural abundance	5 %

7 3D HNCACB

Instrument

Bruker Avance - 600 MHz with cryoprobe

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 (±0.1) K, pH 7 (±0.05), Details Rv0899(73-220) in 5 mM phosphate, pH 7.0

#	Name	Isotope labeling	Concentration
1	Rv0899(73-220)	[U-99% 15N]	1.7 (±0.1) mM
2	Rv0899(73-220)	[U-99% 13C; U-99% 15N]	1 (±0.1) mM
3	phosphate	natural abundance	5 mM
4	H2O	natural abundance	95 %
5	D2O	natural abundance	5 %

8 3D C(CO)NH

Instrument

Bruker Avance - 600 MHz with cryoprobe

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 (±0.1) K, pH 7 (±0.05), Details Rv0899(73-220) in 5 mM phosphate, pH 7.0

#	Name	Isotope labeling	Concentration
1	Rv0899(73-220)	[U-99% 15N]	1.7 (±0.1) mM
2	Rv0899(73-220)	[U-99% 13C; U-99% 15N]	1 (±0.1) mM
3	phosphate	natural abundance	5 mM
4	H2O	natural abundance	95 %
5	D2O	natural abundance	5 %

9 3D HCCH-TOCSY

Instrument

Bruker Avance - 600 MHz with cryoprobe

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 313 (±0.1) K, pH 7 (±0.05), Details Rv0899(73-220) in 5 mM phosphate, pH 7.0

#	Name	Isotope labeling	Concentration
6	Rv0899(73-220)	[U-99% 13C; U-99% 15N]	1 (±0.1) mM
7	Rv0899(73-220)	[U-99% 15N]	0.3 (±0.1) mM
8	D2O	natural abundance	100 %
9	phosphate	natural abundance	5 mM

10 3D 1H-15N NOESY

Instrument

Bruker Avance - 600 MHz with cryoprobe

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 (±0.1) K, pH 7 (±0.05), Details Rv0899(73-220) in 5 mM phosphate, pH 7.0

#	Name	Isotope labeling	Concentration
1	Rv0899(73-220)	[U-99% 15N]	1.7 (±0.1) mM
2	Rv0899(73-220)	[U-99% 13C; U-99% 15N]	1 (±0.1) mM
3	phosphate	natural abundance	5 mM
4	H2O	natural abundance	95 %
5	D2O	natural abundance	5 %

11 3D 1H-15N TOCSY

Instrument

Bruker Avance - 600 MHz with cryoprobe

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 (±0.1) K, pH 7 (±0.05), Details Rv0899(73-220) in 5 mM phosphate, pH 7.0

#	Name	Isotope labeling	Concentration
1	Rv0899(73-220)	[U-99% 15N]	1.7 (±0.1) mM
2	Rv0899(73-220)	[U-99% 13C; U-99% 15N]	1 (±0.1) mM
3	phosphate	natural abundance	5 mM
4	H2O	natural abundance	95 %
5	D2O	natural abundance	5 %

12 3D 1H-13C NOESY

Instrument

Bruker Avance - 600 MHz with cryoprobe

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 (±0.1) K, pH 7 (±0.05), Details Rv0899(73-220) in 5 mM phosphate, pH 7.0

#	Name	Isotope labeling	Concentration
1	Rv0899(73-220)	[U-99% 15N]	1.7 (±0.1) mM
2	Rv0899(73-220)	[U-99% 13C; U-99% 15N]	1 (±0.1) mM
3	phosphate	natural abundance	5 mM
4	H2O	natural abundance	95 %
5	D2O	natural abundance	5 %

13 3D HNCO

Instrument

Bruker Avance - 600 MHz with cryoprobe

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 (±0.1) K, pH 7 (±0.05), Details Rv0899(73-220) in 5 mM phosphate, pH 7.0

#	Name	Isotope labeling	Concentration
1	Rv0899(73-220)	[U-99% 15N]	1.7 (±0.1) mM
2	Rv0899(73-220)	[U-99% 13C; U-99% 15N]	1 (±0.1) mM
3	phosphate	natural abundance	5 mM
4	H2O	natural abundance	95 %
5	D2O	natural abundance	5 %

NMR combined restraints 3 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints	combined_16846_2ksm.nef
Input source #2: Coordindates	2ksm.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

------80--------90-------100-------110-------120-------130-------140-------150-------160-------170--
GASALSLSLLSISRSGNTVTLIGDFPDEAAKAALMTALNGLLAPGVNVIDQIHVDPVVRSLDFSSAEPVFTASVPIPDFGLKVERDTVTLTGTAPSSEHK
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
GASALSLSLLSISRSGNTVTLIGDFPDEAAKAALMTALNGLLAPGVNVIDQIHVDPVVRSLDFSSAEPVFTASVPIPDFGLKVERDTVTLTGTAPSSEHK
--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100

-----180-------190-------200---
DAVKRAATSTWPDMKIVNNIEVTGQAPPGPP
|||||||||||||||||||||||||||||||
DAVKRAATSTWPDMKIVNNIEVTGQAPPGPP
-------110-------120-------130-

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	131	0	0	100.0

Content subtype: combined_16846_2ksm.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	1248		95.4 (chain: A, length: 131)
1	Distance restraints	CNS/XPLOR_distance_constraints_2	Warning	1552 (1)	noe	92.4 (chain: A, length: 131)

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 131, Sequence coverage: 95.4%
- Total number of assignments
  - ¹³C chemical shifts: 511
  - ¹H chemical shifts: 619
  - ¹⁵N chemical shifts: 118
- Completeness of assignments
  - Entity_assemble_ID: A
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
89	ASN	CG	176.334
123	GLN	CD	180.313
191	ASN	CG	176.387
197	GLN	CD	180.647

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	565	507	89.7
¹H chemical shifts	720	619	86.0
¹⁵N chemical shifts	131	118	90.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	262	234	89.3
¹H chemical shifts	259	236	91.1
¹⁵N chemical shifts	119	111	93.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	303	273	90.1
¹H chemical shifts	461	383	83.1
¹⁵N chemical shifts	12	7	58.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	92	88	95.7
¹H chemical shifts	92	87	94.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	29	15	51.7
¹H chemical shifts	30	22	73.3
¹⁵N chemical shifts	1	1	100.0

Distance restraints

Saveframe: CNS/XPLOR_distance_constraints_2
- Status: Warning
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 131, Sequence coverage: 92.4%
- Total number of restraints: 1552
- Weight of restraints: 1.0 (1552)
- Potential type of restraints: square-well-parabolic (1552)
- Range of distance target values: 2.55, 4.0 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
- Distance restraints on contact map

Keywords BON domain, Membrane protein, mycobacterium tuberculosis, OmpATb

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (0.898 seconds)

BMRB: 16846

Sample #1

Sample #2

Sample #3

Sample #4

Sample #5

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. Rv0899, : 1 : 4 : 1 entities Detail

Experiments performed 13 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

NMR combined restraints 3 contents Detail